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Protein

Sensor histidine kinase GraS

Gene

graS

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system GraR/GraS involved in resistance against cationic antimicrobial peptides (CAMPs). GraS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to GraR (By similarity).By similarity

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance, Two-component regulatory system, Virulence

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase GraS (EC:2.7.13.3)
Alternative name(s):
Glycopeptide resistance-associated protein S
Gene namesi
Name:graS
Ordered Locus Names:NWMN_0629
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006386 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Transmembranei43 – 63HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003479241 – 346Sensor histidine kinase GraSAdd BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei129Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliA6QEW9.
SMRiA6QEW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 332Histidine kinasePROSITE-ProRule annotationAdd BLAST207

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000032012.
KOiK19077.
OMAiAYLYSAI.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QEW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNLKWVAYF LKSRMNWIFW ILFLNFLMLG ISLIDYDFPI DSLFYIVSLN
60 70 80 90 100
LSLTMIFLLL TYFKEVKLYK HFDKDKEIEE IKHKDLAETP FQRHTVDYLY
110 120 130 140 150
RQISAHKEKV VEQQLQLNMH EQTITEFVHD IKTPVTAMKL LIDQEKNQER
160 170 180 190 200
KQALLYEWSR INSMLDTQLY ITRLESQRKD MYFDYVSLKR MVIDEIQLTR
210 220 230 240 250
HISQVKGIGF DVDFKVDDYV YTDIKWCRMI IRQILSNALK YSENFNIEIG
260 270 280 290 300
TELNDQHVSL YIKDYGRGIS KKDMPRIFER GFTSTANRNE TTSSGMGLYL
310 320 330 340
VNSVKDQLGI HLQVTSTVGK GTTVRLIFPL QNEIVERMSE VTNLSF
Length:346
Mass (Da):41,079
Last modified:August 21, 2007 - v1
Checksum:i50820220FB5FDEAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA. Translation: BAF66901.1.
RefSeqiWP_001061252.1. NC_009641.1.

Genome annotation databases

EnsemblBacteriaiBAF66901; BAF66901; NWMN_0629.
GeneIDi28379047.
KEGGisae:NWMN_0629.
PATRICi19584711. VBIStaAur133992_0681.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA. Translation: BAF66901.1.
RefSeqiWP_001061252.1. NC_009641.1.

3D structure databases

ProteinModelPortaliA6QEW9.
SMRiA6QEW9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF66901; BAF66901; NWMN_0629.
GeneIDi28379047.
KEGGisae:NWMN_0629.
PATRICi19584711. VBIStaAur133992_0681.

Phylogenomic databases

HOGENOMiHOG000032012.
KOiK19077.
OMAiAYLYSAI.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRAS_STAAE
AccessioniPrimary (citable) accession number: A6QEW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.