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Protein

N-acetylneuraminate lyase

Gene

nanA

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.UniRule annotation

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.UniRule annotation

Pathwayi: N-acetylneuraminate degradation

This protein is involved in step 1 of the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. N-acetylneuraminate lyase (nanA)
  2. no protein annotated in this organism
  3. Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE)
  4. no protein annotated in this organism
  5. Glucosamine-6-phosphate deaminase (nagB)
This subpathway is part of the pathway N-acetylneuraminate degradation, which is itself part of Amino-sugar metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate, the pathway N-acetylneuraminate degradation and in Amino-sugar metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei137Involved in proton transfer during cleavageUniRule annotation1
Active sitei165Schiff-base intermediate with substrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processCarbohydrate metabolism
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00629; UER00680

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyaseUniRule annotation (EC:4.1.3.3UniRule annotation)
Short name:
NALUniRule annotation
Short name:
Neu5Ac lyaseUniRule annotation
Alternative name(s):
N-acetylneuraminate pyruvate-lyaseUniRule annotation
N-acetylneuraminic acid aldolaseUniRule annotation
Sialate lyaseUniRule annotation
Sialic acid aldolaseUniRule annotation
Sialic acid lyaseUniRule annotation
Gene namesi
Name:nanAUniRule annotation
Ordered Locus Names:NWMN_0257
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006386 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000731711 – 293N-acetylneuraminate lyaseAdd BLAST293

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6QDU7
SMRiA6QDU7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 49Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000173608
KOiK01639
OMAiLYEYNQC

Family and domain databases

CDDicd00954 NAL, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01237 N_acetylneuram_lyase, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR002220 DapA-like
IPR005264 NanA
IPR020625 Schiff_base-form_aldolases_AS
PANTHERiPTHR42849 PTHR42849, 1 hit
PfamiView protein in Pfam
PF00701 DHDPS, 1 hit
PIRSFiPIRSF001365 DHDPS, 1 hit
PRINTSiPR00146 DHPICSNTHASE
SMARTiView protein in SMART
SM01130 DHDPS, 1 hit
PROSITEiView protein in PROSITE
PS00666 DHDPS_2, 1 hit

Sequencei

Sequence statusi: Complete.

A6QDU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKDLKGLYA ALLVPFDENG QVNEQGLKQI AQNAIETEEL DGLYVNGSSG
60 70 80 90 100
ENFLLNTEQK KQVFKVAKEA VGDKVKLIAQ VGSLDLNEAI ELGKYATELG
110 120 130 140 150
YDALSAVTPF YYPFTFEEIR DYYFDIIEAT QNNMIIYAIP DLTGVNISIE
160 170 180 190 200
QFSELFNHEK IVGVKYTAPN FFLLERIRKA FPDKLILSGF DEMLVQATIS
210 220 230 240 250
GVDGAIGSTY NVNGRRARKI FDLARQGQIQ EAYQLQHDSN DIIETVLSMG
260 270 280 290
IYPTLKEILR HRGIDAGLPK RPFKPFNEAH RQTLDQLIAK YDL
Length:293
Mass (Da):33,043
Last modified:August 21, 2007 - v1
Checksum:i62D7DEFFDC1AF431
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA Translation: BAF66529.1
RefSeqiWP_001030738.1, NZ_CP023390.1

Genome annotation databases

EnsemblBacteriaiBAF66529; BAF66529; NWMN_0257
KEGGisae:NWMN_0257

Similar proteinsi

Entry informationi

Entry nameiNANA_STAAE
AccessioniPrimary (citable) accession number: A6QDU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health