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A6QDL6 (LDH1_STAAE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-lactate dehydrogenase 1

Short name=L-LDH 1
EC=1.1.1.27
Gene names
Name:ldh1
Ordered Locus Names:NWMN_0176
OrganismStaphylococcus aureus (strain Newman) [Complete proteome] [HAMAP]
Taxonomic identifier426430 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesStaphylococcus

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Appears to be the primary factor that allows S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells. Ref.2

Catalytic activity

(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488

Subunit structure

Homotetramer By similarity. HAMAP MF_00488

Subcellular location

Cytoplasm By similarity HAMAP MF_00488.

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
   Biological processGlycolysis
Stress response
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-lactate dehydrogenase activity

Inferred from electronic annotation. Source: EC

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317L-lactate dehydrogenase 1 HAMAP MF_00488
PRO_0000343842

Regions

Nucleotide binding15 – 4329NAD By similarity

Sites

Active site1791Proton acceptor By similarity
Binding site921Substrate By similarity
Binding site1241NAD or substrate By similarity
Binding site1551Substrate By similarity
Binding site2321Substrate By similarity

Amino acid modifications

Modified residue2231Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
A6QDL6 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: CDDE481269B7DE56

FASTA31734,583
        10         20         30         40         50         60 
MNKFKGNKVV LIGNGAVGSS YAFSLVNQSI VDELVIIDLD TEKVRGDVMD LKHATPYSPT 

        70         80         90        100        110        120 
TVRVKAGEYS DCHDADLVVI CAGAAQKPGE TRLDLVSKNL KIFKSIVGEV MASKFDGIFL 

       130        140        150        160        170        180 
VATNPVDILA YATWKFSGLP KERVIGSGTI LDSARFRLLL SEAFDVAPRS VDAQIIGEHG 

       190        200        210        220        230        240 
DTELPVWSHA NIAGQPLKTL LEQRPEGKAQ IEQIFVQTRD AAYDIIQAKG ATYYGVAMGL 

       250        260        270        280        290        300 
ARITEAIFRN EDAVLTVSAL LEGEYEEEDV YIGVPAVINR NGIRNVVEIP LNDEEQSKFA 

       310 
HSAKTLKDIM AEAEELK 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands."
Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.
J. Bacteriol. 190:300-310(2008) [PubMed: 17951380] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Newman.
[2]"A nitric oxide-inducible lactate dehydrogenase enables Staphylococcus aureus to resist innate immunity."
Richardson A.R., Libby S.J., Fang F.C.
Science 319:1672-1676(2008) [PubMed: 18356528] [Abstract]
Cited for: FUNCTION IN NITROSATIVE STRESS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009351 Genomic DNA. Translation: BAF66448.1.
RefSeqYP_001331211.1. NC_009641.1.

3D structure databases

ProteinModelPortalA6QDL6.
SMRA6QDL6. Positions 1-313.
ModBaseSearch...

Protein-protein interaction databases

STRINGA6QDL6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000031912; EBSTAP00000030782; EBSTAG00000031910.
GeneID5330031.
GenomeReviewsGene locus NWMN_0176 in contig AP009351_GR.
KEGGsae:NWMN_0176.
PATRIC19583690. VBIStaAur133992_0188.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
GeneTreeEBGT00050000024431.
HOGENOMHBG566126.
OMAMDLNHGV.
ProtClustDBCLSK884508.

Enzyme and pathway databases

BioCycSAUR426430:NWMN_0176-MONOMER.

Family and domain databases

HAMAPMF_00488. Lactate_dehydrog.
[Tree]
InterProIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00016.
PANTHERPTHR11540:SF3. PTHR11540:SF3. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01771. L-LDH-NAD. 1 hit.
PROSITEPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLDH1_STAAE
AccessionPrimary (citable) accession number: A6QDL6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: August 21, 2007
Last modified: December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families