A6QDL6 (LDH1_STAAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase 1
Short name=L-LDH 1
EC=1.1.1.27

Gene names

Name:	ldh1
Ordered Locus Names:	NWMN_0176

Organism

Staphylococcus aureus (strain Newman) [Complete proteome] [HAMAP]

Taxonomic identifier

426430 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Appears to be the primary factor that allows S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells. Ref.2
Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis Stress response
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	L-lactate dehydrogenase 1 HAMAP MF_00488		PRO_0000343842
Regions
	Nucleotide binding	15 – 43	29	NAD By similarity
Sites
	Active site	179	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	124	1	NAD or substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
Amino acid modifications
	Modified residue	223	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6QDL6 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: CDDE481269B7DE56	FASTA	317	34,583
	10 20 30 40 50 60 MNKFKGNKVV LIGNGAVGSS YAFSLVNQSI VDELVIIDLD TEKVRGDVMD LKHATPYSPT 70 80 90 100 110 120 TVRVKAGEYS DCHDADLVVI CAGAAQKPGE TRLDLVSKNL KIFKSIVGEV MASKFDGIFL 130 140 150 160 170 180 VATNPVDILA YATWKFSGLP KERVIGSGTI LDSARFRLLL SEAFDVAPRS VDAQIIGEHG 190 200 210 220 230 240 DTELPVWSHA NIAGQPLKTL LEQRPEGKAQ IEQIFVQTRD AAYDIIQAKG ATYYGVAMGL 250 260 270 280 290 300 ARITEAIFRN EDAVLTVSAL LEGEYEEEDV YIGVPAVINR NGIRNVVEIP LNDEEQSKFA 310 HSAKTLKDIM AEAEELK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands." Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K. J. Bacteriol. 190:300-310(2008) [PubMed: 17951380] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Newman.
[2]	"A nitric oxide-inducible lactate dehydrogenase enables Staphylococcus aureus to resist innate immunity." Richardson A.R., Libby S.J., Fang F.C. Science 319:1672-1676(2008) [PubMed: 18356528] [Abstract] Cited for: FUNCTION IN NITROSATIVE STRESS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009351 Genomic DNA. Translation: BAF66448.1.
RefSeq	YP_001331211.1. NC_009641.1.
3D structure databases
ProteinModelPortal	A6QDL6.
SMR	A6QDL6. Positions 1-313.
ModBase	Search...
Protein-protein interaction databases
STRING	A6QDL6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000031912; EBSTAP00000030782; EBSTAG00000031910.
GeneID	5330031.
GenomeReviews	Gene locus NWMN_0176 in contig AP009351_GR.
KEGG	sae:NWMN_0176.
PATRIC	19583690. VBIStaAur133992_0188.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
GeneTree	EBGT00050000024431.
HOGENOM	HBG566126.
OMA	MDLNHGV.
ProtClustDB	CLSK884508.
Enzyme and pathway databases
BioCyc	SAUR426430:NWMN_0176-MONOMER.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00016.
PANTHER	PTHR11540:SF3. PTHR11540:SF3. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH1_STAAE

Accession

Primary (citable) accession number: A6QDL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program