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A6QB84

- SYI_SULNB

UniProt

A6QB84 - SYI_SULNB

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfurovum sp. (strain NBC37-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
Binding sitei612 – 6121ATPUniRule annotation
Metal bindingi894 – 8941ZincUniRule annotation
Metal bindingi897 – 8971ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi912 – 9121ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSP387093:GH25-1819-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SUN_1796
OrganismiSulfurovum sp. (strain NBC37-1)
Taxonomic identifieri387093 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaSulfurovum
ProteomesiUP000006378: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Isoleucine--tRNA ligasePRO_1000022134Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi387093.SUN_1796.

Structurei

3D structure databases

ProteinModelPortaliA6QB84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QB84-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDFKETLLLP KTDFPMRGNL PANEPKKYKT WFDTNIYEQM KAKREGAELF
60 70 80 90 100
TLHDGPPYAN GDIHIGHALN KILKDIILKY NYFQGKAVRM TPGWDCHGLP
110 120 130 140 150
IEQKVEEKLG KSKKEAMPTE KFRELCRAHA GKFVDIQRDE FKSLGVVADW
160 170 180 190 200
ENPYVTMDFK FEANIYRTLC EVAKRGLLVE RHKPIFWSWA ARTALADAEV
210 220 230 240 250
EYEDKEDYSI YVHFELSDAA KEKLGLEGKA GLVIWTTTPW TLPANTGISI
260 270 280 290 300
NPDEMYVLTD DGHIVADARY DAMIEEGVVA GHASRKIAAT ELDGLLAINP
310 320 330 340 350
LNERPSKVVL GEHVMMDGGT GCVHTAPGHG EDDYKVGLEN GLEVVMPVDE
360 370 380 390 400
RGCYDESVVG LDLLPDAEKF VGMHIFKANE PILELLGDNL LKVSKFTHSY
410 420 430 440 450
PHCWRTKKPL IYRATNQWFI SIDDAAKGSD KTLREAAVDA IDSVDFYPAS
460 470 480 490 500
SKNRLKPMVE GRPDWCISRQ RSWGVPIAFF RVKSTKAVIF DEDVLEHVAS
510 520 530 540 550
LFDEHGADAW YSMSIAELLP AGSKYDPADL EKIEDILDVW FDSGSTWNSV
560 570 580 590 600
LSSGNYDAGN YPASLYLEGS DQHRGWFQSS LLLSSAINGI APYETIITHG
610 620 630 640 650
FTMDAKGEKM SKSKGNVVAP EKVVKQFGSE ILRLWVALSD YQNDQKISDD
660 670 680 690 700
ILKQTAEQYR KIRNTFRFLL ANVNDLDALV SADAYGELDR WILNKADDVF
710 720 730 740 750
ASVKESFETY DFLKGFATLN HFITNELSGI YMDVTKDRLY CEAKDSDVRR
760 770 780 790 800
ATQSAMALIS KAMLGLIAPV LTYTADEILA YAPAIFKGDI ENVFDLVYEA
810 820 830 840 850
VPETAASFDD AILLEAREKF SEAIDSLKKE KVIKATLELE IAGDRDLLPI
860 870 880 890 900
SDDKDLEDWF VVSAVKESSK GEQVASFKVE GRTFTVHKAM MAKCPRCWRF
910
TSTSEDCLCE RCAKVVGA
Length:918
Mass (Da):103,033
Last modified:August 21, 2007 - v1
Checksum:iC36014E919AD2338
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009179 Genomic DNA. Translation: BAF72743.1.
RefSeqiWP_012083554.1. NC_009663.1.
YP_001359100.1. NC_009663.1.

Genome annotation databases

EnsemblBacteriaiBAF72743; BAF72743; SUN_1796.
GeneIDi5363362.
KEGGisun:SUN_1796.
PATRICi23776607. VBISulSp49917_1824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009179 Genomic DNA. Translation: BAF72743.1 .
RefSeqi WP_012083554.1. NC_009663.1.
YP_001359100.1. NC_009663.1.

3D structure databases

ProteinModelPortali A6QB84.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 387093.SUN_1796.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAF72743 ; BAF72743 ; SUN_1796 .
GeneIDi 5363362.
KEGGi sun:SUN_1796.
PATRICi 23776607. VBISulSp49917_1824.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SSP387093:GH25-1819-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
    Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
    Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBC37-1.

Entry informationi

Entry nameiSYI_SULNB
AccessioniPrimary (citable) accession number: A6QB84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3