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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfurovum sp. (strain NBC37-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
Binding sitei612 – 6121ATPUniRule annotation
Metal bindingi894 – 8941ZincUniRule annotation
Metal bindingi897 – 8971ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi912 – 9121ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSP387093:GH25-1819-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SUN_1796
OrganismiSulfurovum sp. (strain NBC37-1)
Taxonomic identifieri387093 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaSulfurovum
ProteomesiUP000006378 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Isoleucine--tRNA ligasePRO_1000022134Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi387093.SUN_1796.

Structurei

3D structure databases

ProteinModelPortaliA6QB84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QB84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFKETLLLP KTDFPMRGNL PANEPKKYKT WFDTNIYEQM KAKREGAELF
60 70 80 90 100
TLHDGPPYAN GDIHIGHALN KILKDIILKY NYFQGKAVRM TPGWDCHGLP
110 120 130 140 150
IEQKVEEKLG KSKKEAMPTE KFRELCRAHA GKFVDIQRDE FKSLGVVADW
160 170 180 190 200
ENPYVTMDFK FEANIYRTLC EVAKRGLLVE RHKPIFWSWA ARTALADAEV
210 220 230 240 250
EYEDKEDYSI YVHFELSDAA KEKLGLEGKA GLVIWTTTPW TLPANTGISI
260 270 280 290 300
NPDEMYVLTD DGHIVADARY DAMIEEGVVA GHASRKIAAT ELDGLLAINP
310 320 330 340 350
LNERPSKVVL GEHVMMDGGT GCVHTAPGHG EDDYKVGLEN GLEVVMPVDE
360 370 380 390 400
RGCYDESVVG LDLLPDAEKF VGMHIFKANE PILELLGDNL LKVSKFTHSY
410 420 430 440 450
PHCWRTKKPL IYRATNQWFI SIDDAAKGSD KTLREAAVDA IDSVDFYPAS
460 470 480 490 500
SKNRLKPMVE GRPDWCISRQ RSWGVPIAFF RVKSTKAVIF DEDVLEHVAS
510 520 530 540 550
LFDEHGADAW YSMSIAELLP AGSKYDPADL EKIEDILDVW FDSGSTWNSV
560 570 580 590 600
LSSGNYDAGN YPASLYLEGS DQHRGWFQSS LLLSSAINGI APYETIITHG
610 620 630 640 650
FTMDAKGEKM SKSKGNVVAP EKVVKQFGSE ILRLWVALSD YQNDQKISDD
660 670 680 690 700
ILKQTAEQYR KIRNTFRFLL ANVNDLDALV SADAYGELDR WILNKADDVF
710 720 730 740 750
ASVKESFETY DFLKGFATLN HFITNELSGI YMDVTKDRLY CEAKDSDVRR
760 770 780 790 800
ATQSAMALIS KAMLGLIAPV LTYTADEILA YAPAIFKGDI ENVFDLVYEA
810 820 830 840 850
VPETAASFDD AILLEAREKF SEAIDSLKKE KVIKATLELE IAGDRDLLPI
860 870 880 890 900
SDDKDLEDWF VVSAVKESSK GEQVASFKVE GRTFTVHKAM MAKCPRCWRF
910
TSTSEDCLCE RCAKVVGA
Length:918
Mass (Da):103,033
Last modified:August 21, 2007 - v1
Checksum:iC36014E919AD2338
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009179 Genomic DNA. Translation: BAF72743.1.
RefSeqiWP_012083554.1. NC_009663.1.

Genome annotation databases

EnsemblBacteriaiBAF72743; BAF72743; SUN_1796.
KEGGisun:SUN_1796.
PATRICi23776607. VBISulSp49917_1824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009179 Genomic DNA. Translation: BAF72743.1.
RefSeqiWP_012083554.1. NC_009663.1.

3D structure databases

ProteinModelPortaliA6QB84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi387093.SUN_1796.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF72743; BAF72743; SUN_1796.
KEGGisun:SUN_1796.
PATRICi23776607. VBISulSp49917_1824.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciSSP387093:GH25-1819-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
    Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
    Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBC37-1.

Entry informationi

Entry nameiSYI_SULNB
AccessioniPrimary (citable) accession number: A6QB84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: July 22, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.