SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A6QB84

- SYI_SULNB

UniProt

A6QB84 - SYI_SULNB

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene
ileS, SUN_1796
Organism
Sulfurovum sp. (strain NBC37-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylate By similarity
Binding sitei612 – 6121ATP By similarity
Metal bindingi894 – 8941Zinc By similarity
Metal bindingi897 – 8971Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi912 – 9121Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSP387093:GH25-1819-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SUN_1796
OrganismiSulfurovum sp. (strain NBC37-1)
Taxonomic identifieri387093 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaSulfurovum
ProteomesiUP000006378: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Isoleucine--tRNA ligaseUniRule annotationPRO_1000022134Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi387093.SUN_1796.

Structurei

3D structure databases

ProteinModelPortaliA6QB84.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi609 – 6135"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QB84-1 [UniParc]FASTAAdd to Basket

« Hide

MDFKETLLLP KTDFPMRGNL PANEPKKYKT WFDTNIYEQM KAKREGAELF    50
TLHDGPPYAN GDIHIGHALN KILKDIILKY NYFQGKAVRM TPGWDCHGLP 100
IEQKVEEKLG KSKKEAMPTE KFRELCRAHA GKFVDIQRDE FKSLGVVADW 150
ENPYVTMDFK FEANIYRTLC EVAKRGLLVE RHKPIFWSWA ARTALADAEV 200
EYEDKEDYSI YVHFELSDAA KEKLGLEGKA GLVIWTTTPW TLPANTGISI 250
NPDEMYVLTD DGHIVADARY DAMIEEGVVA GHASRKIAAT ELDGLLAINP 300
LNERPSKVVL GEHVMMDGGT GCVHTAPGHG EDDYKVGLEN GLEVVMPVDE 350
RGCYDESVVG LDLLPDAEKF VGMHIFKANE PILELLGDNL LKVSKFTHSY 400
PHCWRTKKPL IYRATNQWFI SIDDAAKGSD KTLREAAVDA IDSVDFYPAS 450
SKNRLKPMVE GRPDWCISRQ RSWGVPIAFF RVKSTKAVIF DEDVLEHVAS 500
LFDEHGADAW YSMSIAELLP AGSKYDPADL EKIEDILDVW FDSGSTWNSV 550
LSSGNYDAGN YPASLYLEGS DQHRGWFQSS LLLSSAINGI APYETIITHG 600
FTMDAKGEKM SKSKGNVVAP EKVVKQFGSE ILRLWVALSD YQNDQKISDD 650
ILKQTAEQYR KIRNTFRFLL ANVNDLDALV SADAYGELDR WILNKADDVF 700
ASVKESFETY DFLKGFATLN HFITNELSGI YMDVTKDRLY CEAKDSDVRR 750
ATQSAMALIS KAMLGLIAPV LTYTADEILA YAPAIFKGDI ENVFDLVYEA 800
VPETAASFDD AILLEAREKF SEAIDSLKKE KVIKATLELE IAGDRDLLPI 850
SDDKDLEDWF VVSAVKESSK GEQVASFKVE GRTFTVHKAM MAKCPRCWRF 900
TSTSEDCLCE RCAKVVGA 918
Length:918
Mass (Da):103,033
Last modified:August 21, 2007 - v1
Checksum:iC36014E919AD2338
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009179 Genomic DNA. Translation: BAF72743.1.
RefSeqiWP_012083554.1. NC_009663.1.
YP_001359100.1. NC_009663.1.

Genome annotation databases

EnsemblBacteriaiBAF72743; BAF72743; SUN_1796.
GeneIDi5363362.
KEGGisun:SUN_1796.
PATRICi23776607. VBISulSp49917_1824.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009179 Genomic DNA. Translation: BAF72743.1 .
RefSeqi WP_012083554.1. NC_009663.1.
YP_001359100.1. NC_009663.1.

3D structure databases

ProteinModelPortali A6QB84.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 387093.SUN_1796.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAF72743 ; BAF72743 ; SUN_1796 .
GeneIDi 5363362.
KEGGi sun:SUN_1796.
PATRICi 23776607. VBISulSp49917_1824.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SSP387093:GH25-1819-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
    Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
    Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBC37-1.

Entry informationi

Entry nameiSYI_SULNB
AccessioniPrimary (citable) accession number: A6QB84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi