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Protein

Glutamate 5-kinase

Gene

proB

Organism
Sulfurovum sp. (strain NBC37-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathway:iL-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71ATPUniRule annotation
Binding sitei46 – 461SubstrateUniRule annotation
Binding sitei131 – 1311SubstrateUniRule annotation
Binding sitei143 – 1431Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi163 – 1642ATPUniRule annotation
Nucleotide bindingi205 – 2117ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSP387093:GH25-1804-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:SUN_1781
OrganismiSulfurovum sp. (strain NBC37-1)
Taxonomic identifieri387093 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaSulfurovum
ProteomesiUP000006378 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Glutamate 5-kinasePRO_1000081116Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi387093.SUN_1781.

Structurei

3D structure databases

ProteinModelPortaliA6QB69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OMAiDYGMRCA.
OrthoDBiEOG6PGK7G.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QB69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIVIKVGS HVLTEDGSIA RERMMALVEL IADLKKHSYE VALVSSGAVA
60 70 80 90 100
AGYTQLPLDR DDIANRQALA AIGQPLLLKM YQEKFARFDL LCSQVLFSAD
110 120 130 140 150
VFESDKHVSH AKVAIDTLLA NNVVPIINEN DTVSIEELVF GDNDRLSAHV
160 170 180 190 200
AHYFDADLLV ILSDIDALYD KDPNCFQDAR RRAVVNEIEE EELAAEHTPN
210 220 230 240 250
NEFATGGIVT KLQAADFLMK HGREMFLASG FELSDVKSFL IEGEHKGGTL

FTSTKRS
Length:257
Mass (Da):28,450
Last modified:August 21, 2007 - v1
Checksum:i23BDF2CBB0E4DDE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009179 Genomic DNA. Translation: BAF72728.1.
RefSeqiWP_012083538.1. NC_009663.1.

Genome annotation databases

EnsemblBacteriaiBAF72728; BAF72728; SUN_1781.
KEGGisun:SUN_1781.
PATRICi23776575. VBISulSp49917_1808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009179 Genomic DNA. Translation: BAF72728.1.
RefSeqiWP_012083538.1. NC_009663.1.

3D structure databases

ProteinModelPortaliA6QB69.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi387093.SUN_1781.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF72728; BAF72728; SUN_1781.
KEGGisun:SUN_1781.
PATRICi23776575. VBISulSp49917_1808.

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OMAiDYGMRCA.
OrthoDBiEOG6PGK7G.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciSSP387093:GH25-1804-MONOMER.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
    Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
    Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBC37-1.

Entry informationi

Entry nameiPROB_SULNB
AccessioniPrimary (citable) accession number: A6QB69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: July 22, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.