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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Sulfurovum sp. (strain NBC37-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-tRNA ligase activity Source: UniProtKB-EC
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSP387093:GH25-1153-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2 (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name:
GluRS 2
Gene namesi
Name:gltX2
Ordered Locus Names:SUN_1138
OrganismiSulfurovum sp. (strain NBC37-1)
Taxonomic identifieri387093 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaSulfurovum
ProteomesiUP000006378 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Glutamate--tRNA ligase 2PRO_0000367778Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi387093.SUN_1138.

Structurei

3D structure databases

ProteinModelPortaliA6Q9D4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi6 – 1611"HIGH" regionAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiFRYHDAW.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A6Q9D4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRFAPSPTG DMRTEQLRIA IFNYIVAKQK EVNFIVRIED TDKERNITGK
60 70 80 90 100
DTEILQILEK FAITHDSVFH QSEHLNIHQT LAIRLLEEGK AFVCTCTPEA
110 120 130 140 150
PESDKTPSRY NGKCFNVDKE ELKRLKEEKI PFVIRLKKPE HDMIIHDLYK
160 170 180 190 200
GETVTSADEV DSFVILRADA TPTENFASAC DDMLSGIDFI IRSEEHLDET
210 220 230 240 250
AKQEYVKKQL GYEEETTYAH LPVILNEEGQ KMDEKDDAFT VKWLFEEGYI
260 270 280 290 300
PDAIANYLIS LGNTTPTDIF TLPEAIEWFN ITKLSGSPVK FNIEELRLLN
310 320 330 340 350
RKHLEKMDDK RLSSLFGFAD ADIGKLAKLY INEAATINEL DARIKAIFSP
360 370 380 390 400
KDFDGKWGEQ MRMLEKIIAE APMFATFEAF ESHLMKESGL SGEYFSKPLR
410 420
VLLTGAEQGP ELSDIYPYIK SYLLEVAS
Length:428
Mass (Da):48,962
Last modified:August 21, 2007 - v1
Checksum:i57A3F687C6A48033
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009179 Genomic DNA. Translation: BAF72093.1.
RefSeqiWP_011980826.1. NC_009663.1.
YP_001358450.1. NC_009663.1.

Genome annotation databases

EnsemblBacteriaiBAF72093; BAF72093; SUN_1138.
KEGGisun:SUN_1138.
PATRICi23775243. VBISulSp49917_1150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009179 Genomic DNA. Translation: BAF72093.1.
RefSeqiWP_011980826.1. NC_009663.1.
YP_001358450.1. NC_009663.1.

3D structure databases

ProteinModelPortaliA6Q9D4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi387093.SUN_1138.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF72093; BAF72093; SUN_1138.
KEGGisun:SUN_1138.
PATRICi23775243. VBISulSp49917_1150.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiFRYHDAW.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciSSP387093:GH25-1153-MONOMER.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
    Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
    Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBC37-1.

Entry informationi

Entry nameiSYE2_SULNB
AccessioniPrimary (citable) accession number: A6Q9D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 21, 2007
Last modified: April 1, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.