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A6Q8W3 (A6Q8W3_SULNB) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Lipoyl synthase HAMAP-Rule MF_00206
EC=2.8.1.8 HAMAP-Rule MF_00206
Alternative name(s):
Lip-syn HAMAP-Rule MF_00206
Lipoate synthase HAMAP-Rule MF_00206
Lipoic acid synthase HAMAP-Rule MF_00206
Sulfur insertion protein LipA HAMAP-Rule MF_00206
Gene names
Name:lipA HAMAP-Rule MF_00206 EMBL BAF71922.1
Ordered Locus Names:SUN_0965 EMBL BAF71922.1
OrganismSulfurovum sp. (strain NBC37-1) [Complete proteome] [HAMAP] EMBL BAF71922.1
Taxonomic identifier387093 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Sulfurovum

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP-Rule MF_00206

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm By similarity SAAS SAAS003698 HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family. HAMAP-Rule MF_00206

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding391Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding441Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding501Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding651Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding691Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding721Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206

Sequences

Sequence LengthMass (Da)Tools
A6Q8W3 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 150E7D3FC20B1211

FASTA28632,175
        10         20         30         40         50         60 
MSTQKAAYSK PEWLRKKITL SSLREVEAII AKGKLHTVCQ EAMCPNIGEC FSRKQATFLI 

        70         80         90        100        110        120 
LGKECTRHCT FCNVSKEMPL PPDPDEPLHV AESVEAMGLR HVVITSPTRD DLSDGGAEHF 

       130        140        150        160        170        180 
AKVVRAIKTY DETIIVELLI PDLQENEAAL QIIAQSGAQI IGHNLETVPR LYHIRKGAKY 

       190        200        210        220        230        240 
ERSLRVLKKL AQLNPDIRTK SGIMLGLGEK EEEVNALLQD LLDHNCRLLS IGQYLSPSNE 

       250        260        270        280 
HTKVVEFVPP ERFDHFHDVG MAMGFDFIKS SPYTRSSYMA DEYLKS

« Hide

References

[1]"Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.L., Takai K., Horikoshi K.
Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBC37-1 EMBL BAF71922.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP009179 Genomic DNA. Translation: BAF71922.1.
RefSeqYP_001358279.1. NC_009663.1.

3D structure databases

ProteinModelPortalA6Q8W3.
ModBaseSearch...

Protein-protein interaction databases

STRING387093.SUN_0965.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF71922; BAF71922; SUN_0965.
GeneID5362526.
KEGGsun:SUN_0965.
PATRIC23774889. VBISulSp49917_0973.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0320.
HOGENOMHOG000235997.
KOK03644.
OMAARCPNIT.
ProtClustDBPRK05481.

Enzyme and pathway databases

BioCycSSP387093:GH25-980-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameA6Q8W3_SULNB
AccessionPrimary (citable) accession number: A6Q8W3
Entry history
Integrated into UniProtKB/TrEMBL: August 21, 2007
Last sequence update: August 21, 2007
Last modified: May 29, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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