A6Q639 (PYRE_NITSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	NIS_1844

Organism

Nitratiruptor sp. (strain SB155-2) [Complete proteome] [HAMAP]

Taxonomic identifier

387092 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Nitratiruptor

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000066263
Regions
	Region	113 – 121	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	117	1	Orotate By similarity
	Binding site	145	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6Q639 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: F08BF2E154B5A865	FASTA	202	21,795
	10 20 30 40 50 60 MDIEKIYKES GALLKGHFLL SSGKHSPNYL QSAKVLEDPK KAELLAKELA KQIQAAGIEV 70 80 90 100 110 120 DTVCSPAIGG LLAGYELARA LGVRFIFTER KDGKMTLRRG FEVEPGEKVL ICEDIITTGG 130 140 150 160 170 180 SAMEAAKEME KRGAEVVAFA ALANRGVCQR TGSEIPSKSE CKLPSNKTLF ALADFTFPIY 190 200 EPKECPMCAE GSEPIKPGSR GN

References

[1]

"Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens." Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K. Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed: 17615243] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SB155-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009178 Genomic DNA. Translation: BAF70948.1.
RefSeq	YP_001357305.1. NC_009662.1.
3D structure databases
ProteinModelPortal	A6Q639.
ModBase	Search...
Protein-protein interaction databases
STRING	A6Q639.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5360000.
GenomeReviews	Gene locus NIS_1844 in contig AP009178_GR.
KEGG	nis:NIS_1844.
PATRIC	22685880. VBINitSp82229_1930.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0461.
HOGENOM	HBG404341.
OMA	FYLQSAK.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	NSP387092:NIS_1844-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR023031. Orotate_PribosylTferase. IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. PyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_NITSB

Accession

Primary (citable) accession number: A6Q639

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program