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A6Q604 (NAPA_NITSB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:NIS_1808
OrganismNitratiruptor sp. (strain SB155-2) [Complete proteome] [HAMAP]
Taxonomic identifier387092 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaNitratiruptor

Protein attributes

Sequence length936 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 936905Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000069721

Regions

Domain40 – 96574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A6Q604 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 7171B3121E2C1E10

FASTA936106,390
        10         20         30         40         50         60 
MALSRRDFLK SSAAAAAASA VGLSVPKEVE AASKEAQKGW RWDKAVCRFC GTGCGIMIAT 

        70         80         90        100        110        120 
KDDRIVAVKG DPLAPVNRGL NCIKGYFTAK IMYGADRLKT PLLRMNDKGE FDKKGKFRPV 

       130        140        150        160        170        180 
SWKRAFDEME KQFRKAYNEL GPTGVAFFGS GQYTVMEGYA AAKLMKAGFR SNNIDPNARH 

       190        200        210        220        230        240 
CMASAVAGFI QTFGIDEPAG CYDDIELTDT IVLWGSNMAE MHPILWARCT DRKLSDPNKV 

       250        260        270        280        290        300 
KVVVLSTYTH RSCDLADDVI IFKPNTDLAI WNYIARSIVY DHPDAIDWNF VKEYCVFATG 

       310        320        330        340        350        360 
YPDIGYGMRN PKRAEELGYS KKEMQTVWHQ DHKKLSEDEK RALAPFGYGN ADVMKMKHVK 

       370        380        390        400        410        420 
AAGKHWAISF EEFKKSLEPY TLDYVAKVAK GDPDESLESF KAKLQRLKDL YVEKGRKVVS 

       430        440        450        460        470        480 
FWTMGMNQHT RGTWDNELSY VVHFLLGKQA LPGSGAFSLT GQPSACGTAR EVGTFAHRLP 

       490        500        510        520        530        540 
ADMVVFNPKH RAIAEKIWKL PKGTINPKVG SHIVKIMRDL EDGKIKFAWV HVCNPWQDTA 

       550        560        570        580        590        600 
NANHWIKAAR DMDNFIVVSD GYPGISAKVA DLILPSAMIY EKWGAYGNAE RRTQHWRQQV 

       610        620        630        640        650        660 
TPVGDAMPDI WQYTEFAKRF KLKDVWKEWK LPDGTVLPNV LDEAKKMGYS EDDTLFDVLF 

       670        680        690        700        710        720 
ANDYYRSFKW PDPIGEGFLN TEAEGDKRNV IGADGKPWKG YGFFIQKALW EEYRKFGEGR 

       730        740        750        760        770        780 
GHDYAPFDVY HKVRGLRWPV VNGKDTPWRF NVNYDPYAKR EKELGHVKGE FAFYGHALKV 

       790        800        810        820        830        840 
IPQGSLTGPD KNKPKIHLPN KAKIFARPYM EPPEVPDNEY DTWLCTGRVL EHWHSGTMTM 

       850        860        870        880        890        900 
RVPELYRAVP EALCYMHPED AKKRGVKRGD LVVIESRRGK CKARVETRGR NRPPRGLVFV 

       910        920        930 
PWFDERVYIN LVTLDATCPI SKQTDYKKCA VKIYKA 

« Hide

References

[1]"Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SB155-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009178 Genomic DNA. Translation: BAF70913.1.
RefSeqYP_001357270.1. NC_009662.1.

3D structure databases

ProteinModelPortalA6Q604.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING387092.NIS_1808.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF70913; BAF70913; NIS_1808.
GeneID5361706.
KEGGnis:NIS_1808.
PATRIC22685796. VBINitSp82229_1892.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAHPKTRVA.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycNSP387092:GHA5-1856-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_NITSB
AccessionPrimary (citable) accession number: A6Q604
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families