Reviewed, UniProtKB/Swiss-Prot A6Q604 (NAPA_NITSB)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	NIS_1808

Organism

Nitratiruptor sp. (strain SB155-2) [Complete proteome] [HAMAP]

Taxonomic identifier

387092 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Nitratiruptor

Protein attributes

Sequence length	936 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	PeriplasmBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Tat-type signal Potential
	Chain	32 – 936	905	Periplasmic nitrate reductase		PRO_1000069721
Sites
	Metal binding	47	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	50	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	54	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	82	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6Q604-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 7171B3121E2C1E10	FASTA	936	106,390
	10 20 30 40 50 60 MALSRRDFLK SSAAAAAASA VGLSVPKEVE AASKEAQKGW RWDKAVCRFC GTGCGIMIAT 70 80 90 100 110 120 KDDRIVAVKG DPLAPVNRGL NCIKGYFTAK IMYGADRLKT PLLRMNDKGE FDKKGKFRPV 130 140 150 160 170 180 SWKRAFDEME KQFRKAYNEL GPTGVAFFGS GQYTVMEGYA AAKLMKAGFR SNNIDPNARH 190 200 210 220 230 240 CMASAVAGFI QTFGIDEPAG CYDDIELTDT IVLWGSNMAE MHPILWARCT DRKLSDPNKV 250 260 270 280 290 300 KVVVLSTYTH RSCDLADDVI IFKPNTDLAI WNYIARSIVY DHPDAIDWNF VKEYCVFATG 310 320 330 340 350 360 YPDIGYGMRN PKRAEELGYS KKEMQTVWHQ DHKKLSEDEK RALAPFGYGN ADVMKMKHVK 370 380 390 400 410 420 AAGKHWAISF EEFKKSLEPY TLDYVAKVAK GDPDESLESF KAKLQRLKDL YVEKGRKVVS 430 440 450 460 470 480 FWTMGMNQHT RGTWDNELSY VVHFLLGKQA LPGSGAFSLT GQPSACGTAR EVGTFAHRLP 490 500 510 520 530 540 ADMVVFNPKH RAIAEKIWKL PKGTINPKVG SHIVKIMRDL EDGKIKFAWV HVCNPWQDTA 550 560 570 580 590 600 NANHWIKAAR DMDNFIVVSD GYPGISAKVA DLILPSAMIY EKWGAYGNAE RRTQHWRQQV 610 620 630 640 650 660 TPVGDAMPDI WQYTEFAKRF KLKDVWKEWK LPDGTVLPNV LDEAKKMGYS EDDTLFDVLF 670 680 690 700 710 720 ANDYYRSFKW PDPIGEGFLN TEAEGDKRNV IGADGKPWKG YGFFIQKALW EEYRKFGEGR 730 740 750 760 770 780 GHDYAPFDVY HKVRGLRWPV VNGKDTPWRF NVNYDPYAKR EKELGHVKGE FAFYGHALKV 790 800 810 820 830 840 IPQGSLTGPD KNKPKIHLPN KAKIFARPYM EPPEVPDNEY DTWLCTGRVL EHWHSGTMTM 850 860 870 880 890 900 RVPELYRAVP EALCYMHPED AKKRGVKRGD LVVIESRRGK CKARVETRGR NRPPRGLVFV 910 920 930 PWFDERVYIN LVTLDATCPI SKQTDYKKCA VKIYKA

References

[1]

"Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens." Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K. Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed: 17615243] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009178 Genomic DNA. Translation: BAF70913.1.
RefSeq	YP_001357270.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5361706.
GenomeReviews	Gene locus NIS_1808 in contig AP009178_GR.
KEGG	nis:NIS_1808.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_NITSB

Accession

Primary (citable) accession number: A6Q604

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	November 25, 2008
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)