Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A6Q604

- NAPA_NITSB

UniProt

A6Q604 - NAPA_NITSB

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Nitratiruptor sp. (strain SB155-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciNSP387092:GHA5-1856-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:NIS_1808
    OrganismiNitratiruptor sp. (strain SB155-2)
    Taxonomic identifieri387092 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNitratiruptor
    ProteomesiUP000001118: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
    BLAST
    Chaini32 – 936905Periplasmic nitrate reductasePRO_1000069721Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi387092.NIS_1808.

    Structurei

    3D structure databases

    ProteinModelPortaliA6Q604.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHPKTRVA.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF10518. TAT_signal. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A6Q604-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALSRRDFLK SSAAAAAASA VGLSVPKEVE AASKEAQKGW RWDKAVCRFC    50
    GTGCGIMIAT KDDRIVAVKG DPLAPVNRGL NCIKGYFTAK IMYGADRLKT 100
    PLLRMNDKGE FDKKGKFRPV SWKRAFDEME KQFRKAYNEL GPTGVAFFGS 150
    GQYTVMEGYA AAKLMKAGFR SNNIDPNARH CMASAVAGFI QTFGIDEPAG 200
    CYDDIELTDT IVLWGSNMAE MHPILWARCT DRKLSDPNKV KVVVLSTYTH 250
    RSCDLADDVI IFKPNTDLAI WNYIARSIVY DHPDAIDWNF VKEYCVFATG 300
    YPDIGYGMRN PKRAEELGYS KKEMQTVWHQ DHKKLSEDEK RALAPFGYGN 350
    ADVMKMKHVK AAGKHWAISF EEFKKSLEPY TLDYVAKVAK GDPDESLESF 400
    KAKLQRLKDL YVEKGRKVVS FWTMGMNQHT RGTWDNELSY VVHFLLGKQA 450
    LPGSGAFSLT GQPSACGTAR EVGTFAHRLP ADMVVFNPKH RAIAEKIWKL 500
    PKGTINPKVG SHIVKIMRDL EDGKIKFAWV HVCNPWQDTA NANHWIKAAR 550
    DMDNFIVVSD GYPGISAKVA DLILPSAMIY EKWGAYGNAE RRTQHWRQQV 600
    TPVGDAMPDI WQYTEFAKRF KLKDVWKEWK LPDGTVLPNV LDEAKKMGYS 650
    EDDTLFDVLF ANDYYRSFKW PDPIGEGFLN TEAEGDKRNV IGADGKPWKG 700
    YGFFIQKALW EEYRKFGEGR GHDYAPFDVY HKVRGLRWPV VNGKDTPWRF 750
    NVNYDPYAKR EKELGHVKGE FAFYGHALKV IPQGSLTGPD KNKPKIHLPN 800
    KAKIFARPYM EPPEVPDNEY DTWLCTGRVL EHWHSGTMTM RVPELYRAVP 850
    EALCYMHPED AKKRGVKRGD LVVIESRRGK CKARVETRGR NRPPRGLVFV 900
    PWFDERVYIN LVTLDATCPI SKQTDYKKCA VKIYKA 936
    Length:936
    Mass (Da):106,390
    Last modified:August 21, 2007 - v1
    Checksum:i7171B3121E2C1E10
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009178 Genomic DNA. Translation: BAF70913.1.
    RefSeqiWP_012083176.1. NC_009662.1.
    YP_001357270.1. NC_009662.1.

    Genome annotation databases

    EnsemblBacteriaiBAF70913; BAF70913; NIS_1808.
    GeneIDi5361706.
    KEGGinis:NIS_1808.
    PATRICi22685796. VBINitSp82229_1892.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009178 Genomic DNA. Translation: BAF70913.1 .
    RefSeqi WP_012083176.1. NC_009662.1.
    YP_001357270.1. NC_009662.1.

    3D structure databases

    ProteinModelPortali A6Q604.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 387092.NIS_1808.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAF70913 ; BAF70913 ; NIS_1808 .
    GeneIDi 5361706.
    KEGGi nis:NIS_1808.
    PATRICi 22685796. VBINitSp82229_1892.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HPKTRVA.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci NSP387092:GHA5-1856-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF10518. TAT_signal. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
      Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
      Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SB155-2.

    Entry informationi

    Entry nameiNAPA_NITSB
    AccessioniPrimary (citable) accession number: A6Q604
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3