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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Nitratiruptor sp. (strain SB155-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciNSP387092:GHA5-1856-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:NIS_1808
OrganismiNitratiruptor sp. (strain SB155-2)
Taxonomic identifieri387092 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNitratiruptor
ProteomesiUP000001118 Componenti: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
BLAST
Chaini32 – 936905Periplasmic nitrate reductasePRO_1000069721Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi387092.NIS_1808.

Structurei

3D structure databases

ProteinModelPortaliA6Q604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiKITWDKA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6Q604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSRRDFLK SSAAAAAASA VGLSVPKEVE AASKEAQKGW RWDKAVCRFC
60 70 80 90 100
GTGCGIMIAT KDDRIVAVKG DPLAPVNRGL NCIKGYFTAK IMYGADRLKT
110 120 130 140 150
PLLRMNDKGE FDKKGKFRPV SWKRAFDEME KQFRKAYNEL GPTGVAFFGS
160 170 180 190 200
GQYTVMEGYA AAKLMKAGFR SNNIDPNARH CMASAVAGFI QTFGIDEPAG
210 220 230 240 250
CYDDIELTDT IVLWGSNMAE MHPILWARCT DRKLSDPNKV KVVVLSTYTH
260 270 280 290 300
RSCDLADDVI IFKPNTDLAI WNYIARSIVY DHPDAIDWNF VKEYCVFATG
310 320 330 340 350
YPDIGYGMRN PKRAEELGYS KKEMQTVWHQ DHKKLSEDEK RALAPFGYGN
360 370 380 390 400
ADVMKMKHVK AAGKHWAISF EEFKKSLEPY TLDYVAKVAK GDPDESLESF
410 420 430 440 450
KAKLQRLKDL YVEKGRKVVS FWTMGMNQHT RGTWDNELSY VVHFLLGKQA
460 470 480 490 500
LPGSGAFSLT GQPSACGTAR EVGTFAHRLP ADMVVFNPKH RAIAEKIWKL
510 520 530 540 550
PKGTINPKVG SHIVKIMRDL EDGKIKFAWV HVCNPWQDTA NANHWIKAAR
560 570 580 590 600
DMDNFIVVSD GYPGISAKVA DLILPSAMIY EKWGAYGNAE RRTQHWRQQV
610 620 630 640 650
TPVGDAMPDI WQYTEFAKRF KLKDVWKEWK LPDGTVLPNV LDEAKKMGYS
660 670 680 690 700
EDDTLFDVLF ANDYYRSFKW PDPIGEGFLN TEAEGDKRNV IGADGKPWKG
710 720 730 740 750
YGFFIQKALW EEYRKFGEGR GHDYAPFDVY HKVRGLRWPV VNGKDTPWRF
760 770 780 790 800
NVNYDPYAKR EKELGHVKGE FAFYGHALKV IPQGSLTGPD KNKPKIHLPN
810 820 830 840 850
KAKIFARPYM EPPEVPDNEY DTWLCTGRVL EHWHSGTMTM RVPELYRAVP
860 870 880 890 900
EALCYMHPED AKKRGVKRGD LVVIESRRGK CKARVETRGR NRPPRGLVFV
910 920 930
PWFDERVYIN LVTLDATCPI SKQTDYKKCA VKIYKA
Length:936
Mass (Da):106,390
Last modified:August 20, 2007 - v1
Checksum:i7171B3121E2C1E10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009178 Genomic DNA. Translation: BAF70913.1.
RefSeqiWP_012083176.1. NC_009662.1.
YP_001357270.1. NC_009662.1.

Genome annotation databases

EnsemblBacteriaiBAF70913; BAF70913; NIS_1808.
KEGGinis:NIS_1808.
PATRICi22685796. VBINitSp82229_1892.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009178 Genomic DNA. Translation: BAF70913.1.
RefSeqiWP_012083176.1. NC_009662.1.
YP_001357270.1. NC_009662.1.

3D structure databases

ProteinModelPortaliA6Q604.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi387092.NIS_1808.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF70913; BAF70913; NIS_1808.
KEGGinis:NIS_1808.
PATRICi22685796. VBINitSp82229_1892.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiKITWDKA.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciNSP387092:GHA5-1856-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
    Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
    Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SB155-2.

Entry informationi

Entry nameiNAPA_NITSB
AccessioniPrimary (citable) accession number: A6Q604
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 4, 2008
Last sequence update: August 20, 2007
Last modified: March 31, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.