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A6Q512 (SYL_NITSB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:NIS_1464
OrganismNitratiruptor sp. (strain SB155-2) [Complete proteome] [HAMAP]
Taxonomic identifier387092 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaNitratiruptor

Protein attributes

Sequence length815 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

leucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

arginine-tRNA ligase activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 815815Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009382

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif583 – 5875"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5861ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6Q512 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 48FB5009F2996E89

FASTA81594,146
        10         20         30         40         50         60 
MQYDPKAIEQ KWQNEWKEKN AFEPQENYSK EKMYVLSMFP YPSGRIHMGH VRNYTIGDAI 

        70         80         90        100        110        120 
ARYYRKTGAN VLHPIGWDAF GMPAENAAIK HKVHPKKWTY ENIDYMRKEL DALGLSFSHD 

       130        140        150        160        170        180 
REFATCDPLY SKWEQSFIID MWNRGLLYRK KAAVNWCPHD KTVLANEQVI EGRCWRCDTE 

       190        200        210        220        230        240 
VVQKEIEQYF LKITDYAQEL LEDLKKLEGN WPNQVIAMQR NWIGRSEGLE FRLHFDETSA 

       250        260        270        280        290        300 
KKAGIDGFEV FTTRPDTIYG VTYTALAPEH PVVKHLIETK QLSDEAVQKI CTMQNQNART 

       310        320        330        340        350        360 
RQQAEKEGLF LDLYVIHPLT KQKIPVWVAN FVLAEYGSGA VMAVPAHDER DFEFAHKYNL 

       370        380        390        400        410        420 
PIKYIIKPKE GELDTTKAYT EPGILFDSGE FSGFESSEAK QKIIEYFEEN GIGKRSVNYK 

       430        440        450        460        470        480 
LKDWLVSRQR YWGTPIPLIK CPKCGIVPEK KENLPVTLPE DVEITGEGNP LELHPTWKKT 

       490        500        510        520        530        540 
TCPKCGGEAE RETDTLDTFV ESSWYFLRYT TPRKYWEEVP FRKEDTDYWM PVDQYIGGIE 

       550        560        570        580        590        600 
HAILHLLYAR FFTKVLRDLG YVNLDEPFKR LLTQGMVLKD GAKMSKSKGN TVDPDEIVAK 

       610        620        630        640        650        660 
FGADTARLFI LFAAPPAKEL EWSDSAVEGA YRFIKRFFER SQNAYKTKSL PKIDQKSLSK 

       670        680        690        700        710        720 
EEKEARKKVY EALQKSTDVY TKSFSFNTLI AASMEALNAL NGQNNPDIWT EGYWVLTNIL 

       730        740        750        760        770        780 
EPIIPHTCWE ISHNLFERNN FTRLQLDPAA LEEDSVTLAV TVNGKRRAEI EVPKDASKEE 

       790        800        810 
ILAKAKEIAK KWIDGKTIVK EIVVPGRLVN IVVKG 

« Hide

References

[1]"Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SB155-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009178 Genomic DNA. Translation: BAF70571.1.
RefSeqYP_001356928.1. NC_009662.1.

3D structure databases

ProteinModelPortalA6Q512.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING387092.NIS_1464.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF70571; BAF70571; NIS_1464.
GeneID5360666.
KEGGnis:NIS_1464.
PATRIC22685078. VBINitSp82229_1536.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycNSP387092:GHA5-1509-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR001278. Arg-tRNA-ligase.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_NITSB
AccessionPrimary (citable) accession number: A6Q512
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries