Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nitratiruptor sp. (strain SB155-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNSP387092:GHA5-1322-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NIS_1282
OrganismiNitratiruptor sp. (strain SB155-2)
Taxonomic identifieri387092 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNitratiruptor
ProteomesiUP000001118 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Isoleucine--tRNA ligasePRO_1000022095Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi387092.NIS_1282.

Structurei

3D structure databases

ProteinModelPortaliA6Q4I1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6Q4I1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLLLP KTTFPMRGNL PQNEPKRFAK WFEKDVYEKM KKSREGKELF
60 70 80 90 100
TLHDGPPYAN GHIHIGHALN KILKDIIVKF NYFEGKAVRF TPGWDCHGLP
110 120 130 140 150
IEQQVEKKLG TAKKEQLPKT KIRELCREHA AKFVGIQKEE FKNLGVIADW
160 170 180 190 200
EKPYLTMDYA FEADIYRALC EIAKEGLLVE RSKPVYWSWA AKTALAEAEV
210 220 230 240 250
EYEDKVSPSI YVAFKLDKEA VQKIGKEASI IIWTTTPWTL PANTGIALNP
260 270 280 290 300
DIEYVLTSDG YVVAADLLDE LKEKGIVKGD VEKSISPKDL ENLHAINPLN
310 320 330 340 350
GRRSRIVLGE HVTTESGTGA VHTAPGHGED DYRVGLKYDL EVLMPVDDAG
360 370 380 390 400
RYDETIVREK LLPEEFVGMN VFEANDKICE LLGDALLKKE DIKHSYPHCW
410 420 430 440 450
RTHKPIIFRA TKQWFIAVDK APKDLQKTLR QIALEEVEKT TFYPEWGRNR
460 470 480 490 500
LKSMIENRPD WCISRQRDWG VPIAFFRNKK TGEVIYDEKV LNYIAMIFER
510 520 530 540 550
MGTDAWYSLS VEELLYPGSG YDPNDLEKVM DILDVWFDSG STWYAVLKSR
560 570 580 590 600
RYDAGNYPAD LYLEGSDQHR GWFQSSLLVS GAIEKRAPFK AILTHGFTVD
610 620 630 640 650
EKGEKMSKSK GNVVAPMDVA KKYGVEILRL WVAMSDYQSD LKISDNILKQ
660 670 680 690 700
IAEQYRKLRN TFRFMLANIN DLETIQSDFG VLDRWILAKA KSVFEEVEKQ
710 720 730 740 750
FKNYQFAKGF SALNNFIVNE FSGIYLDVCK DRLYCDALND SHRRASQSAM
760 770 780 790 800
ALIAKSMLGL IAPVLTYTAD EIVEHAPSVL RNDAEDIFDF AIEPLPEIQS
810 820 830 840 850
DFDEVYMIEA RSKFNEIVDQ LKKKKIIKSS LELVIETTSS KVLALDATER
860 870 880 890 900
EDWFIVSGVE EEIGSKELGD FKVEGDQFII KEAILHKCPR CWKYKAKEEG
910
ALCERCQKVV DGLEQAGS
Length:918
Mass (Da):105,037
Last modified:August 20, 2007 - v1
Checksum:iD813A33F9C6F77E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009178 Genomic DNA. Translation: BAF70390.1.
RefSeqiWP_012082653.1. NC_009662.1.
YP_001356747.1. NC_009662.1.

Genome annotation databases

EnsemblBacteriaiBAF70390; BAF70390; NIS_1282.
KEGGinis:NIS_1282.
PATRICi22684680. VBINitSp82229_1342.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009178 Genomic DNA. Translation: BAF70390.1.
RefSeqiWP_012082653.1. NC_009662.1.
YP_001356747.1. NC_009662.1.

3D structure databases

ProteinModelPortaliA6Q4I1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi387092.NIS_1282.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF70390; BAF70390; NIS_1282.
KEGGinis:NIS_1282.
PATRICi22684680. VBINitSp82229_1342.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciNSP387092:GHA5-1322-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
    Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
    Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SB155-2.

Entry informationi

Entry nameiSYI_NITSB
AccessioniPrimary (citable) accession number: A6Q4I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 14, 2008
Last sequence update: August 20, 2007
Last modified: March 31, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.