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A6Q3Z7 (DDL_NITSB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:NIS_1097
OrganismNitratiruptor sp. (strain SB155-2) [Complete proteome] [HAMAP]
Taxonomic identifier387092 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaNitratiruptor

Protein attributes

Sequence length342 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 342342D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074779

Regions

Domain132 – 326195ATP-grasp
Nucleotide binding159 – 21052ATP By similarity

Sites

Metal binding2821Magnesium or manganese 1 By similarity
Metal binding2941Magnesium or manganese 1 By similarity
Metal binding2941Magnesium or manganese 2 By similarity
Metal binding2961Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A6Q3Z7 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 8C442237BE583032

FASTA34238,808
        10         20         30         40         50         60 
MQYAVVFGGK SYEHEISIVS TIAIKDIIPG AIFIFLDGNR DFYLIEKADL KSNYFSSGNY 

        70         80         90        100        110        120 
KKSPKLELKK GGFYQKSLLK EKKIPADVVI NLVHGADGED GKLASLLEFF EIDYIGPRIE 

       130        140        150        160        170        180 
GSVISYSKLL TKLYAKECGI EVLPYQLLRK QDKKIIDFEY PVIIKPNHLG SSIGVSVVYD 

       190        200        210        220        230        240 
SSELEYALDV AFEFDDEVLI EPFIEGIEEY NLAGAKGQTF HFSKIEAVKK EKLLDFEKKY 

       250        260        270        280        290        300 
LDFGRSGEVK DASLNETLRL HIRNAFEKIY DPLFSGAIIR IDFFVRDGKL YLNEINPVPG 

       310        320        330        340 
SLANYLFGDF RAVLEDVAKH LPKSKNIVID YRYINSIQSA KK 

« Hide

References

[1]"Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SB155-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009178 Genomic DNA. Translation: BAF70206.1.
RefSeqYP_001356563.1. NC_009662.1.

3D structure databases

ProteinModelPortalA6Q3Z7.
SMRA6Q3Z7. Positions 1-322.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING387092.NIS_1097.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF70206; BAF70206; NIS_1097.
GeneID5359906.
KEGGnis:NIS_1097.
PATRIC22684290. VBINitSp82229_1149.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000102494.
KOK01921.
OMAKYMDFSR.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycNSP387092:GHA5-1136-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_NITSB
AccessionPrimary (citable) accession number: A6Q3Z7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways