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A6Q3R4

- SYV_NITSB

UniProt

A6Q3R4 - SYV_NITSB

Protein

Valine--tRNA ligase

Gene

valS

Organism
Nitratiruptor sp. (strain SB155-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.UniRule annotation

    Catalytic activityi

    ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei537 – 5371ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. valine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. valyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciNSP387092:GHA5-1052-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Valine--tRNA ligaseUniRule annotation (EC:6.1.1.9UniRule annotation)
    Alternative name(s):
    Valyl-tRNA synthetaseUniRule annotation
    Short name:
    ValRSUniRule annotation
    Gene namesi
    Name:valSUniRule annotation
    Ordered Locus Names:NIS_1013
    OrganismiNitratiruptor sp. (strain SB155-2)
    Taxonomic identifieri387092 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaNitratiruptor
    ProteomesiUP000001118: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 871871Valine--tRNA ligasePRO_1000022170Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi387092.NIS_1013.

    Structurei

    3D structure databases

    ProteinModelPortaliA6Q3R4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili805 – 87167UniRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi47 – 5711"HIGH" regionAdd
    BLAST
    Motifi534 – 5385"KMSKS" region

    Domaini

    ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.UniRule annotation
    The C-terminal coiled-coil domain is crucial for aminoacylation activity.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.UniRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0525.
    HOGENOMiHOG000020093.
    KOiK01873.
    OMAiTEEHDCG.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.287.380. 1 hit.
    1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02004. Val_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR010978. tRNA-bd_arm.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR019499. Val-tRNA_synth_tRNA-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR002303. Valyl-tRNA_ligase.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF10458. Val_tRNA-synt_C. 1 hit.
    [Graphical view]
    PRINTSiPR00986. TRNASYNTHVAL.
    SUPFAMiSSF46589. SSF46589. 1 hit.
    SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00422. valS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6Q3R4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKATKYDPK KVERQFYQIW ETRGYFETDG NKKIQNGKTF CIMMPPPNVT    50
    GRLHIGHALT FTLQDIMVRY KRMDGYETLW QPGTDHAGIA TQNVVEKQLL 100
    SKGIKKEEIG REKFLEYVWK WKEESGNAIV TQLRLLGVSP AWSRERFTMD 150
    KGLKNAVREA FVNLYYEGLI VKGNYMINWC THDGALSDIE VEYEEKEGAL 200
    YHIKYPIVGS DEYLVVATTR PETYFGDTAV MVNPNDERYK HLIGKKVRLP 250
    LINREIPIIA DEHVDMEFGT GAVKVTPAHD PNDYEVGKRH NLPFITIFDE 300
    NGILNEEAGE FAGIERLEAR KKVVEKLEQE GFIEKIEPHK HQVGHCYRCG 350
    NVVEPYISPQ WFVKAEIAKE AVKKANEGET KFYPPQWLNN FNAWMRELRD 400
    WCISRQLWWG HRIPVWYCRA CGHEWASKKE HEESCPKCGS TDIYQDPDVL 450
    DTWFSSALWP FSTLGWGNGD WGKGVKWFED DLKKFYPNDL LITGFDILFF 500
    WVARMMMMGE HFLHKLPFKD VYLHALVRDE HGQKMSKSRG NVIDPIDTIE 550
    EYSADALRFT LAALAVQGRD IRLSKERLEL YRNFTNKLYN AARFLQIHQE 600
    KFDDLQNIQI KTDLGKYILS RFGLAIQEVR NNLNSYRFND AATTLYRFLW 650
    GEFCDWGIEL SKVNKDAIAE LGAIFKESMK LLHPFMPFIT EFLYQELSGT 700
    SIEENESIMI QPYPKAAPID EEIMKRFETI IDAIVSIRRA KALIDMANKT 750
    IPKVLIKGDL EESAKAYISK LAKVETIEFV SEPAENAVTD IGNYVEVFIP 800
    LEGIDLTPIL NRLNKQKEKL QKEIDKLSRM LSNENFVKNA PQAVVEQNRA 850
    ALAEAQNRLA TIEEELARLT R 871
    Length:871
    Mass (Da):100,866
    Last modified:August 21, 2007 - v1
    Checksum:i74A573E74706C8E7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009178 Genomic DNA. Translation: BAF70123.1.
    RefSeqiWP_012082386.1. NC_009662.1.
    YP_001356480.1. NC_009662.1.

    Genome annotation databases

    EnsemblBacteriaiBAF70123; BAF70123; NIS_1013.
    GeneIDi5360927.
    KEGGinis:NIS_1013.
    PATRICi22684104. VBINitSp82229_1056.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009178 Genomic DNA. Translation: BAF70123.1 .
    RefSeqi WP_012082386.1. NC_009662.1.
    YP_001356480.1. NC_009662.1.

    3D structure databases

    ProteinModelPortali A6Q3R4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 387092.NIS_1013.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAF70123 ; BAF70123 ; NIS_1013 .
    GeneIDi 5360927.
    KEGGi nis:NIS_1013.
    PATRICi 22684104. VBINitSp82229_1056.

    Phylogenomic databases

    eggNOGi COG0525.
    HOGENOMi HOG000020093.
    KOi K01873.
    OMAi TEEHDCG.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci NSP387092:GHA5-1052-MONOMER.

    Family and domain databases

    Gene3Di 1.10.287.380. 1 hit.
    1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02004. Val_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR010978. tRNA-bd_arm.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR019499. Val-tRNA_synth_tRNA-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR002303. Valyl-tRNA_ligase.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF10458. Val_tRNA-synt_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00986. TRNASYNTHVAL.
    SUPFAMi SSF46589. SSF46589. 1 hit.
    SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00422. valS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens."
      Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K.
      Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SB155-2.

    Entry informationi

    Entry nameiSYV_NITSB
    AccessioniPrimary (citable) accession number: A6Q3R4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3