Reviewed, UniProtKB/Swiss-Prot A6Q354 (SYE1_NITSB)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	NIS_0801

Organism

Nitratiruptor sp. (strain SB155-2) [Complete proteome] [HAMAP]

Taxonomic identifier

387092 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Nitratiruptor

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367719
Regions
	Motif	6 – 16	11	"HIGH" region HAMAP MF_00022
	Motif	235 – 239	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	238	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6Q354-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: F340C31FF23E6F72	FASTA	431	49,845
	10 20 30 40 50 60 MIRFAPSPTG DMHIGNLRVA IFNYIEAKKR NERFLIRIED TDIERNIEGK DQEILQILDT 70 80 90 100 110 120 FGLQYDDVVY QSKNFSFHQN FAYKLLNEGK AFACFCTPQE LEKEREQAKK EKRAYRYSGK 130 140 150 160 170 180 CENITLDEAS QRGEPFVVRI KKPTGTIEFD DIIKGHLAFE PNEVDSFVIL RADARPTYNF 190 200 210 220 230 240 ACAVDDMLYD ITLVIRGEDH VSNTPKQIHI RNLLGYDKKI EYAHLPIILN EEGKKMSKRD 250 260 270 280 290 300 KASSVKWLLE EGFLPEAIAN YLILLGNKTP KEIFTLDEAI AWFDLKNISK APAKFDIEKL 310 320 330 340 350 360 RFINRAHLQM MSEEELEKAL GVDEGLGALA KIYLEEASTL KELNTKIDLV LHGKRENETF 370 380 390 400 410 420 REEIETLRNL LKSMELPESF DEFKKELMQK SGLKGKKFFK PLRILLTGSE HGPELSELYP 430 AIKNRIKEVI Q

References

[1]

"Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens." Nakagawa S., Takaki Y., Shimamura S., Reysenbach A.-L., Takai K., Horikoshi K. Proc. Natl. Acad. Sci. U.S.A. 104:12146-12150(2007) [PubMed: 17615243] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009178 Genomic DNA. Translation: BAF69913.1.
RefSeq	YP_001356270.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6Q354.
Genome annotation databases
GeneID	5360157.
GenomeReviews	Gene locus NIS_0801 in contig AP009178_GR.
KEGG	nis:NIS_0801.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	AIFNYIC.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_NITSB

Accession

Primary (citable) accession number: A6Q354

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)