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A6NYG9 (A6NYG9_9FIRM) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_01228
EC=6.1.1.10 HAMAP-Rule MF_01228
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_01228
Gene names
Name:metG HAMAP-Rule MF_01228 EMBL EDM98935.1
ORF Names:BACCAP_03269 EMBL EDM98935.1
OrganismPseudoflavonifractor capillosus ATCC 29799 EMBL EDM98935.1
Taxonomic identifier411467 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Pseudoflavonifractor ›

Protein attributes

Sequence length656 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228 SAAS SAAS023457

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228 SAAS SAAS002547

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01228

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228 SAAS SAAS014758.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily. HAMAP-Rule MF_01228

Contains 1 tRNA-binding domain. HAMAP-Rule MF_01228 SAAS SAAS002547

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data. EMBL EDM98935.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain551 – 656106tRNA-binding By similarity HAMAP-Rule MF_01228
Motif14 – 2411"HIGH" region By similarity HAMAP-Rule MF_01228
Motif301 – 3055"KMSKS" region By similarity HAMAP-Rule MF_01228

Sites

Metal binding1321Zinc By similarity HAMAP-Rule MF_01228
Metal binding1351Zinc By similarity HAMAP-Rule MF_01228
Metal binding1491Zinc By similarity HAMAP-Rule MF_01228
Metal binding1521Zinc By similarity HAMAP-Rule MF_01228

Sequences

Sequence LengthMass (Da)Tools
A6NYG9 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 81BBB82083FDF76F

FASTA65673,793
        10         20         30         40         50         60 
MSNKGKYYIT TPIYYPSDKL HIGHAYCTVA TDVMARYKRL TGYDVMFLTG TDEHGQKIED 

        70         80         90        100        110        120 
KAKDAGVTPK QYVDNIVEGE RGVLDLWKLM NISNDRFIRT TDDYHEAAVQ KIFKQLYEQG 

       130        140        150        160        170        180 
DIYKGTYKGK YCKPCESFWT ESQLVDGKCP DCGREVQDAE EEAYFFRLSK YADRIQDLLE 

       190        200        210        220        230        240 
NTDFLEPRSR VNEMVNNFIK PGLEDLCVSR TSFTWGVPVD FDPGHVVYVW IDALSNYITA 

       250        260        270        280        290        300 
LGYDNDKYND YDKFWPADVH FVGKEIVRFH SIIWPAILMA LNLPLPKKVF GHGWLLLDGG 

       310        320        330        340        350        360 
KMSKSKGNVV DPYLLSERYG VDALRFFLLR TFPFGSDGNF SNEALIGTIN TDLANDLGNL 

       370        380        390        400        410        420 
LSRTVSMVGK YFEGGTLPAE QTADGLDDEL IALASGLRDK YEAAMERFAF QDALSEVFKL 

       430        440        450        460        470        480 
ISRANKYIDE TAPWVLARDE ANKPRLARVM YNLLESLRIS AVLLTPFMPD TSAEILRQIG 

       490        500        510        520        530        540 
ACPCCSQWES AGKWGSLSAT ATVSRGDNLF PRIDMEKELA ALEELEAQAR KAALPAVEVE 

       550        560        570        580        590        600 
PFAEEQVDFD TFCKSDLRAV KVKACENVKK SNKLLKFTLD DGSGTDRQIL SGIAMYYKPE 

       610        620        630        640        650 
ELVGKTLVAI INLPPRKMMG QESCGMLISA VHTEHGEEKL HLLMVDDAIP AGAKLC

« Hide

References

[1]Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M., Thiruvilangam P., Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 29799 EMBL EDM98935.1.
[2]"Draft genome sequence of Pseudoflavonifractor capillosus ATCC 29799."
Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 29799 EMBL EDM98935.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AAXG02000029 Genomic DNA. Translation: EDM98935.1.

3D structure databases

ProteinModelPortalA6NYG9.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEDM98935; EDM98935; BACCAP_03269.
PATRIC31031372. VBIBacCap83047_2493.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA6NYG9_9FIRM
AccessionPrimary (citable) accession number: A6NYG9
Entry history
Integrated into UniProtKB/TrEMBL: August 21, 2007
Last sequence update: August 21, 2007
Last modified: April 3, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info