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Protein

Ubiquitin carboxyl-terminal hydrolase 27

Gene

USP27X

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinase that can reduce the levels of BCL2L11/BIM ubiquitination and stabilize BCL2L11 in response to the RAF-MAPK-degradation signal. By acting on BCL2L11 levels, may counteract the anti-apoptotic effects of MAPK activity.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87NucleophilePROSITE-ProRule annotation1
Active sitei380Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:HS17606-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 27 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 27
Ubiquitin carboxyl-terminal hydrolase 22-like
Ubiquitin thioesterase 27
Ubiquitin-specific-processing protease 27
X-linked ubiquitin carboxyl-terminal hydrolase 27
Gene namesi
Name:USP27X
Synonyms:USP22L, USP27
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:13486. USP27X.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000273820.
PharmGKBiPA134993614.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003063341 – 438Ubiquitin carboxyl-terminal hydrolase 27Add BLAST438

Proteomic databases

MaxQBiA6NNY8.
PRIDEiA6NNY8.

PTM databases

iPTMnetiA6NNY8.
PhosphoSitePlusiA6NNY8.

Expressioni

Gene expression databases

BgeeiENSG00000242013.
CleanExiHS_USP27X.
GenevisibleiA6NNY8. HS.

Interactioni

Subunit structurei

Interacts with phosphorylated BCL2L11 isoform BIMEL; this interaction leads to BCL2L11 deubiquitination and stabilization.By similarity

Protein-protein interaction databases

BioGridi133302. 9 interactors.
IntActiA6NNY8. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliA6NNY8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 421USPAdd BLAST344

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

GeneTreeiENSGT00860000133708.
HOVERGENiHBG058014.
InParanoidiA6NNY8.
KOiK11366.
OMAiTEKHIHE.
OrthoDBiEOG091G09GI.
PhylomeDBiA6NNY8.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6NNY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCKDYVYDKD IEQIAKEEQG EALKLQASTS TEVSHQQCSV PGLGEKFPTW
60 70 80 90 100
ETTKPELELL GHNPRRRRIT SSFTIGLRGL INLGNTCFMN CIVQALTHTP
110 120 130 140 150
ILRDFFLSDR HRCEMPSPEL CLVCEMSSLF RELYSGNPSP HVPYKLLHLV
160 170 180 190 200
WIHARHLAGY RQQDAHEFLI AALDVLHRHC KGDDVGKAAN NPNHCNCIID
210 220 230 240 250
QIFTGGLQSD VTCQACHGVS TTIDPCWDIS LDLPGSCTSF WPMSPGRESS
260 270 280 290 300
VNGESHIPGI TTLTDCLRRF TRPEHLGSSA KIKCGSCQSY QESTKQLTMN
310 320 330 340 350
KLPVVACFHF KRFEHSAKQR RKITTYISFP LELDMTPFMA SSKESRMNGQ
360 370 380 390 400
LQLPTNSGNN ENKYSLFAVV NHQGTLESGH YTSFIRHHKD QWFKCDDAVI
410 420 430
TKASIKDVLD SEGYLLFYHK QVLEHESEKV KEMNTQAY
Length:438
Mass (Da):49,630
Last modified:March 24, 2009 - v3
Checksum:iB6BFD18C62B3774C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32E → D in DR004246 (Ref. 2) Curated1
Sequence conflicti45E → D in DR004246 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF238380 Genomic DNA. No translation available.
DR004246 mRNA. No translation available.
CCDSiCCDS65260.1.
RefSeqiNP_001138545.1. NM_001145073.2.
UniGeneiHs.143587.

Genome annotation databases

EnsembliENST00000621775; ENSP00000483631; ENSG00000273820.
GeneIDi389856.
KEGGihsa:389856.
UCSCiuc033edm.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF238380 Genomic DNA. No translation available.
DR004246 mRNA. No translation available.
CCDSiCCDS65260.1.
RefSeqiNP_001138545.1. NM_001145073.2.
UniGeneiHs.143587.

3D structure databases

ProteinModelPortaliA6NNY8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi133302. 9 interactors.
IntActiA6NNY8. 1 interactor.

PTM databases

iPTMnetiA6NNY8.
PhosphoSitePlusiA6NNY8.

Proteomic databases

MaxQBiA6NNY8.
PRIDEiA6NNY8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000621775; ENSP00000483631; ENSG00000273820.
GeneIDi389856.
KEGGihsa:389856.
UCSCiuc033edm.2. human.

Organism-specific databases

CTDi389856.
GeneCardsiUSP27X.
H-InvDBHIX0077135.
HGNCiHGNC:13486. USP27X.
MIMi300975. gene.
neXtProtiNX_A6NNY8.
OpenTargetsiENSG00000273820.
PharmGKBiPA134993614.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00860000133708.
HOVERGENiHBG058014.
InParanoidiA6NNY8.
KOiK11366.
OMAiTEKHIHE.
OrthoDBiEOG091G09GI.
PhylomeDBiA6NNY8.

Enzyme and pathway databases

BioCyciZFISH:HS17606-MONOMER.

Miscellaneous databases

GenomeRNAii389856.
PROiA6NNY8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000242013.
CleanExiHS_USP27X.
GenevisibleiA6NNY8. HS.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP27_HUMAN
AccessioniPrimary (citable) accession number: A6NNY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 24, 2009
Last modified: November 30, 2016
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Although strongly related to USP22, which deubiquitinates histones, lacks the N-terminal UBP-type zinc finger, suggesting it does not have the ability to deubiquitinate histones.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.