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Protein

Enolase 4

Gene

ENO4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May be required for sperm motility and function.By similarity

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-162eP), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-278), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS)
  2. Phosphoglycerate kinase 2 (PGK2), Phosphoglycerate kinase 1 (PGK1)
  3. no protein annotated in this organism
  4. Alpha-enolase (ENO1), Beta-enolase (ENO3), Enolase 4 (ENO4), Gamma-enolase (ENO2)
  5. Pyruvate kinase, Pyruvate kinase PKM (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase, Pyruvate kinase (PKM), Pyruvate kinase, Pyruvate kinase PKLR (PKLR), Pyruvate kinase, Pyruvate kinase (PKM2), Pyruvate kinase (HEL-S-30), Pyruvate kinase (PKM), Pyruvate kinase (PKM2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei300 – 3001SubstrateBy similarity
Active sitei497 – 4971Proton acceptorBy similarity
Binding sitei548 – 5481SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 4 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
Gene namesi
Name:ENO4
Synonyms:C10orf134
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:31670. ENO4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 628628Enolase 4PRO_0000348454Add
BLAST

Proteomic databases

EPDiA6NNW6.
PaxDbiA6NNW6.
PRIDEiA6NNW6.

Expressioni

Gene expression databases

BgeeiA6NNW6.
ExpressionAtlasiA6NNW6. baseline and differential.

Organism-specific databases

HPAiHPA037938.
HPA061509.

Interactioni

Subunit structurei

Interacts with ENO1 and AKAP4.By similarity

Protein-protein interaction databases

STRINGi9606.ENSP00000345555.

Structurei

3D structure databases

ProteinModelPortaliA6NNW6.
SMRiA6NNW6. Positions 235-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi189 – 23143Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
GeneTreeiENSGT00840000129946.
HOGENOMiHOG000168691.
HOVERGENiHBG107850.
InParanoidiA6NNW6.
PhylomeDBiA6NNW6.
TreeFamiTF354238.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 2 hits.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 3 hits.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A6NNW6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEEGGGRSC GTTRELQKLK QQAMEYYREN DVPRRLEELL NSTFYLQPAD
60 70 80 90 100
VYGHLKANCF SKLAKPPTIC KIVGKDVLDG LGLPTLQVDI FCTIQNFPKN
110 120 130 140 150
VCSVVISTHF EVHENALPEL AKAEEAERAS AVSTAVQWVN STITHELQGM
160 170 180 190 200
APSDQAEVDH LLRIFFASKV QEDKGRKELE KSLEYSTVPT PLPPVPPPPP
210 220 230 240 250
PPPPTKKKGQ KPGRKDTITE KPIAPAEPVE PVLSGSMAIG AVSLAVAKAC
260 270 280 290 300
AMLLNKPLYL NIALLKHNQE QPTTLSMPLL MVSLVSCGKS SSGKLNLMKE
310 320 330 340 350
VICIPHPELT TKQGVEMLME MQKHINKIIE MMPPSPPKAE TKKGHDGSKR
360 370 380 390 400
GQQQITGKMS HLGCLTINCD SIEQPLLLIQ EICANLGLEL GTNLHLAINC
410 420 430 440 450
AGHELMDYNK GKYEVIMGTY KNAAEMVDLY VDLINKYPSI IALIDPFRKE
460 470 480 490 500
DSEQWDSIYH ALGSRCYIIA GTASKSISKL LEQGNISIPK SNGLIIKHTN
510 520 530 540 550
QTTMSDLVEI TNLIDSKKHI TVFGSTEGES SDDSLVDLAV GLGVRFIKLG
560 570 580 590 600
GLSRGERVTK YNRLLTIEEE LVQNGTLGFK EEHTFFYFNE EAEKAAEALE
610 620
AAAAREPLVP TFPTQGVEES AETGASSG
Length:628
Mass (Da):68,821
Last modified:January 15, 2008 - v2
Checksum:iB18C91E2B81A19DA
GO
Isoform 2 (identifier: A6NNW6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-333: Missing.
     409-450: Missing.

Show »
Length:308
Mass (Da):33,793
Checksum:i454F89A2A13D18AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321V → I in BX647301 (PubMed:17974005).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei56 – 333278Missing in isoform 2. 1 PublicationVSP_038263Add
BLAST
Alternative sequencei409 – 45042Missing in isoform 2. 1 PublicationVSP_038264Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX647301 mRNA. No translation available.
AC023283 Genomic DNA. No translation available.
UniGeneiHs.693248.

Genome annotation databases

EnsembliENST00000409522; ENSP00000387194; ENSG00000188316. [A6NNW6-2]
UCSCiuc001lcw.4. human. [A6NNW6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX647301 mRNA. No translation available.
AC023283 Genomic DNA. No translation available.
UniGeneiHs.693248.

3D structure databases

ProteinModelPortaliA6NNW6.
SMRiA6NNW6. Positions 235-571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000345555.

Proteomic databases

EPDiA6NNW6.
PaxDbiA6NNW6.
PRIDEiA6NNW6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409522; ENSP00000387194; ENSG00000188316. [A6NNW6-2]
UCSCiuc001lcw.4. human. [A6NNW6-1]

Organism-specific databases

GeneCardsiENO4.
H-InvDBHIX0026101.
HGNCiHGNC:31670. ENO4.
HPAiHPA037938.
HPA061509.
neXtProtiNX_A6NNW6.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
GeneTreeiENSGT00840000129946.
HOGENOMiHOG000168691.
HOVERGENiHBG107850.
InParanoidiA6NNW6.
PhylomeDBiA6NNW6.
TreeFamiTF354238.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Miscellaneous databases

PROiA6NNW6.

Gene expression databases

BgeeiA6NNW6.
ExpressionAtlasiA6NNW6. baseline and differential.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 2 hits.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 3 hits.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  2. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiENO4_HUMAN
AccessioniPrimary (citable) accession number: A6NNW6
Secondary accession number(s): B8ZZN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: January 15, 2008
Last modified: May 11, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Although it belongs to the enolase family, Leu-362 is present instead of the conserved Glu which is expected to be an active site residue.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.