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Protein

Tubulin polyglutamylase TTLL13P

Gene

TTLL13P

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Polyglutamylase which preferentially modifies alpha-tubulin. Involved in the side-chain elongation step of the polyglutamylation reaction rather than in the initiation step (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei243 – 2431ATPBy similarity
Binding sitei245 – 2451ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi230 – 2334ATP bindingBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin polyglutamylase TTLL13P (EC:6.-.-.-)
Alternative name(s):
Tubulin tyrosine ligase-like family member 13 pseudogene
Tubulin--tyrosine ligase-like protein 13
Gene namesi
Name:TTLL13PImported
Synonyms:TTLL13
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:32484. TTLL13P.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi425 – 4273KRK → EEE: Decreased binding to microtubules and polyglutamylase activity; when associated with 451-E-E-452. 1 Publication
Mutagenesisi451 – 4522RK → EE: Decreased binding to microtubules and polyglutamylase activity; when associated with 425-E--E-427. 1 Publication

Organism-specific databases

PharmGKBiPA143485664.

Polymorphism and mutation databases

BioMutaiTTLL13.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 815815Tubulin polyglutamylase TTLL13PPRO_0000326167Add
BLAST

Proteomic databases

PaxDbiA6NNM8.
PRIDEiA6NNM8.

PTM databases

iPTMnetiA6NNM8.
PhosphoSiteiA6NNM8.

Expressioni

Gene expression databases

BgeeiA6NNM8.
CleanExiHS_TTLL13.

Organism-specific databases

HPAiHPA040133.
HPA041305.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000345294.

Structurei

3D structure databases

ProteinModelPortaliA6NNM8.
SMRiA6NNM8. Positions 87-433.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 430346TTLPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni401 – 48282c-MTBD region1 PublicationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili504 – 52825Sequence analysisAdd
BLAST

Domaini

The flexible c-MTBD (cationic microtubule binding domain) region mediates binding to microtubules. It is positively charged and becomes ordered when bound to microtubules: it interacts with a negatively charged patch on tubulin. The presence of positive charges in the c-MTBD region is essential for proper binding.By similarity1 Publication

Sequence similaritiesi

Belongs to the tubulin--tyrosine ligase family.Sequence analysis
Contains 1 TTL domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2158. Eukaryota.
ENOG410XNWC. LUCA.
HOGENOMiHOG000045569.
HOVERGENiHBG108616.
InParanoidiA6NNM8.
OrthoDBiEOG7RJPQT.
PhylomeDBiA6NNM8.
TreeFamiTF313087.

Family and domain databases

InterProiIPR004344. TTL/TTLL_fam.
[Graphical view]
PfamiPF03133. TTL. 1 hit.
[Graphical view]
PROSITEiPS51221. TTL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: A6NNM8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPSTCRTME SEEDYVEEKE SEKCVKEGVT NPSNSSQQAL LKADYKALKN
60 70 80 90 100
GVPSPIMATK IPKKVIAPVD TGDLEAGRRK RRRKRRSLAI NLTNCKYESV
110 120 130 140 150
RRAAQMCGLK EVGEDEEWTL YWTDCAVSLE RVMDMKRFQK INHFPGMTEI
160 170 180 190 200
CRKDLLARNL NRMYKLYPSE YNIFPRTWCL PADYGDFQSY GRQRKARTYI
210 220 230 240 250
CKPDSGCQGR GIFITRNPRE IKPGEHMICQ QYISKPLLID GFKFDMRVYV
260 270 280 290 300
LITSCDPLRI FTYEEGLARF ATTPYMEPSH NNLDNVCMHL TNYAINKHNE
310 320 330 340 350
NFVRDGAVGS KRKLSTLNIW LQEHSYNPGE LWGDIEDIII KTIISAHSVL
360 370 380 390 400
RHNYRTCFPQ YLNGGTCACF EILGFDILLD HKLKPWLLEV NHSPSFTTDS
410 420 430 440 450
CLDQEVKDAL LCDAMTLVNL RGCDKRKVME EDKRRVKERL FQCYRQPRES
460 470 480 490 500
RKEKTESSHV AMLDQERYED SHLGKYRRIY PGPDTEKYAR FFKHNGSLFQ
510 520 530 540 550
ETAASKAREE CARQQLEEIR LKQEQQETSG TKRQKARDQN QGESAGEKSR
560 570 580 590 600
PRAGLQSLST HLAYRNRNWE KELLPGQLDT MRPQEIVEEE ELERMKALLQ
610 620 630 640 650
RETLIRSLGI VEQLTRLQHP GPQGQKKLHE SRDRLGSQEL KSMSLVLLVL
660 670 680 690 700
LRGAATEQGA PHFLHPVLPH ESIPRILGAL PSMNAAIPHV PRYHLQPKNF
710 720 730 740 750
NWTGEPAAIN SCSLSMKKAG RCYFSSARIR LTSQGQASRR LEAINRVLAG
760 770 780 790 800
SVPPTLTPKQ GYFLQPERVA SDSWTECTLP SMVNSEHRAA KVPLCPASAP
810
MLQRSRALLN INQFR
Note: Gene prediction based on similarity to mouse ortholog.Curated
Length:815
Mass (Da):93,645
Last modified:March 18, 2008 - v2
Checksum:iE9407A6E0DDD9F68
GO
Isoform 2Curated (identifier: A6NNM8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     452-459: KEKTESSH → CARCLACV
     460-815: Missing.

Note: May be due to an intron retention.
Show »
Length:459
Mass (Da):53,222
Checksum:iB6033487ADB06574
GO

Sequence cautioni

The sequence BC036668 differs from that shown. Reason: Frameshift at position 298. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081G → S in AK127965 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti126 – 1261A → T.
Corresponds to variant rs12912620 [ dbSNP | Ensembl ].
VAR_057318
Natural varianti262 – 2621T → I.
Corresponds to variant rs2063743 [ dbSNP | Ensembl ].
VAR_057319

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei452 – 4598KEKTESSH → CARCLACV in isoform 2. 2 PublicationsVSP_052720
Alternative sequencei460 – 815356Missing in isoform 2. 2 PublicationsVSP_052721Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK127965 mRNA. No translation available.
AC091167 Genomic DNA. No translation available.
CH471101 Genomic DNA. Translation: EAX02094.1.
BC036668 Genomic DNA. No translation available.
UniGeneiHs.632164.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK127965 mRNA. No translation available.
AC091167 Genomic DNA. No translation available.
CH471101 Genomic DNA. Translation: EAX02094.1.
BC036668 Genomic DNA. No translation available.
UniGeneiHs.632164.

3D structure databases

ProteinModelPortaliA6NNM8.
SMRiA6NNM8. Positions 87-433.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000345294.

PTM databases

iPTMnetiA6NNM8.
PhosphoSiteiA6NNM8.

Polymorphism and mutation databases

BioMutaiTTLL13.

Proteomic databases

PaxDbiA6NNM8.
PRIDEiA6NNM8.

Protocols and materials databases

DNASUi440307.
Structural Biology KnowledgebaseSearch...

Organism-specific databases

GeneCardsiTTLL13P.
H-InvDBHIX0079716.
HGNCiHGNC:32484. TTLL13P.
HPAiHPA040133.
HPA041305.
neXtProtiNX_A6NNM8.
PharmGKBiPA143485664.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2158. Eukaryota.
ENOG410XNWC. LUCA.
HOGENOMiHOG000045569.
HOVERGENiHBG108616.
InParanoidiA6NNM8.
OrthoDBiEOG7RJPQT.
PhylomeDBiA6NNM8.
TreeFamiTF313087.

Miscellaneous databases

ChiTaRSiTTLL13. human.
NextBioi109140.
PROiA6NNM8.

Gene expression databases

BgeeiA6NNM8.
CleanExiHS_TTLL13.

Family and domain databases

InterProiIPR004344. TTL/TTLL_fam.
[Graphical view]
PfamiPF03133. TTL. 1 hit.
[Graphical view]
PROSITEiPS51221. TTL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  2. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  5. "Multivalent microtubule recognition by tubulin tyrosine ligase-like family glutamylases."
    Garnham C.P., Vemu A., Wilson-Kubalek E.M., Yu I., Szyk A., Lander G.C., Milligan R.A., Roll-Mecak A.
    Cell 161:1112-1123(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, MUTAGENESIS OF 425-LYS--LYS-427 AND 451-ARG-LYS-452.

Entry informationi

Entry nameiTTL13_HUMAN
AccessioniPrimary (citable) accession number: A6NNM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: March 16, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.