A6NKF1 (SAC31_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SAC3 domain-containing protein 1

Alternative name(s):
SAC3 homology domain-containing protein 1

Gene names

Name:	SAC3D1
Synonyms:	SHD1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in centrosome duplication and mitotic progression By similarity.
Subunit structure	May be part of a SHFM1-containing complex.
Subcellular location	Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Note: Localizes on centrosomes in interphase cells and at spindles in mitosis By similarity.
Sequence similarities	Belongs to the SAC3 family.
Caution	It is uncertain whether Met-1 or Met-47 is the initiator.
Sequence caution	The sequence AP003068 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A6NKF1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A6NKF1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     239-404: SVEALHEVLQ...EGTDRPGSPA → ESGSWRLGRA...PARPSARPWR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	SAC3 domain-containing protein 1		PRO_0000308204
Regions
	Compositional bias	8 – 118	111	Pro-rich
Amino acid modifications
	Modified residue	402	1	Phosphoserine Ref.4 Ref.6 Ref.7 Ref.8 Ref.9
Natural variations
	Alternative sequence	239 – 404	166	SVEAL…PGSPA → ESGSWRLGRAWGQEPTMTVE ARWKPCMRFYSCLLPCAPAR PSARPWR in isoform 2.		VSP_036589
	Natural variant	8	1	T → P. Ref.1 Ref.3 Corresponds to variant rs10160811 [ dbSNP | Ensembl ].		VAR_036755
	Natural variant	155	1	L → R. Ref.1 Ref.3 Corresponds to variant rs12271134 [ dbSNP | Ensembl ].		VAR_062213
	Natural variant	186	1	L → P. Corresponds to variant rs3741390 [ dbSNP | Ensembl ].		VAR_036756
Experimental info
	Sequence conflict	181	1	T → A in AAB50210. Ref.1
	Sequence conflict	181	1	T → A in BC007448. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 9B3499AAA23F97B7	FASTA	404	43,553
	10 20 30 40 50 60 MAGRRAQTGS APPRPAAPHP RPASRAFPQH CRPRDAERPP SPRSPLMPGC ELPVGTCPDM 70 80 90 100 110 120 CPAAERAQRE REHRLHRLEV VPGCRQDPPR ADPQRAVKEY SRPAAGKPRP PPSQLRPPSV 130 140 150 160 170 180 LLATVRYLAG EVAESADIAR AEVASFVADR LRAVLLDLAL QGAGDAEAAV VLEAALATLL 190 200 210 220 230 240 TVVARLGPDA ARGPADPVLL QAQVQEGFGS LRRCYARGAG PHPRQPAFQG LFLLYNLGSV 250 260 270 280 290 300 EALHEVLQLP AALRACPPLR KALAVDAAFR EGNAARLFRL LQTLPYLPSC AVQCHVGHAR 310 320 330 340 350 360 REALARFARA FSTPKGQTLP LGFMVNLLAL DGLREARDLC QAHGLPLDGE ERVVFLRGRY 370 380 390 400 VEEGLPPAST CKVLVESKLR GRTLEEVVMA EEEDEGTDRP GSPA
	Isoform 2 [UniParc]. Checksum: 6E23EC439A9A6628	FASTA	285	30,913
	10 20 30 40 50 60 MAGRRAQTGS APPRPAAPHP RPASRAFPQH CRPRDAERPP SPRSPLMPGC ELPVGTCPDM 70 80 90 100 110 120 CPAAERAQRE REHRLHRLEV VPGCRQDPPR ADPQRAVKEY SRPAAGKPRP PPSQLRPPSV 130 140 150 160 170 180 LLATVRYLAG EVAESADIAR AEVASFVADR LRAVLLDLAL QGAGDAEAAV VLEAALATLL 190 200 210 220 230 240 TVVARLGPDA ARGPADPVLL QAQVQEGFGS LRRCYARGAG PHPRQPAFQG LFLLYNLGES 250 260 270 280 GSWRLGRAWG QEPTMTVEAR WKPCMRFYSC LLPCAPARPS ARPWR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Large-scale concatenation cDNA sequencing." Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A. Genome Res. 7:353-358(1997) [PubMed: 9110174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS PRO-8 AND ARG-155. Tissue: Brain.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS PRO-8 AND ARG-155. Tissue: Muscle.
[4]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II." Baillat D., Hakimi M.-A., Naeaer A.M., Shilatifard A., Cooch N., Shiekhattar R. Cell 123:265-276(2005) [PubMed: 16239144] [Abstract] Cited for: IDENTIFICATION IN COMPLEX WITH SHFM1, MASS SPECTROMETRY.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U79266 mRNA. Translation: AAB50210.1. AP003068 Genomic DNA. No translation available. BC007448 mRNA. No translation available.
IPI	IPI00854724. IPI00923502.
UniGene	Hs.23642.
3D structure databases
ProteinModelPortal	A6NKF1.
ModBase	Search...
Protein-protein interaction databases
STRING	A6NKF1.
PTM databases
PhosphoSite	A6NKF1.
Proteomic databases
PRIDE	A6NKF1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000301885; ENSP00000301885; ENSG00000168061.
NMPDR	fig|9606.3.peg.5982.
Organism-specific databases
GeneCards	GC11P064808.
HGNC	HGNC:30179. SAC3D1.
HPA	HPA042677.
neXtProt	NX_A6NKF1.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG06602.
GeneTree	ENSGT00530000063781.
HOGENOM	HBG714632.
HOVERGEN	HBG108455.
InParanoid	A6NKF1.
OrthoDB	EOG4HMJ9Z.
Gene expression databases
Bgee	A6NKF1.
CleanEx	HS_SAC3D1.
Genevestigator	A6NKF1.
Family and domain databases
InterPro	IPR005062. SAC3/GANP/Nin1/mts3/eIF-3-p25. [Graphical view]
Pfam	PF03399. SAC3_GANP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAC31_HUMAN

Accession

Primary (citable) accession number: A6NKF1
Secondary accession number(s): A8MX08, Q99773

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.