A6NK97 (S22AK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 22 member 20

Alternative name(s):
Organic anion transporter 6

Gene names

Name:	SLC22A20
Synonyms:	OAT6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Organic anion transporter that mediates the uptake of estrone sulfate. Inhibited by probenecid, propionate, 2-methylbutyrate, 3-methylbutyrate, benzoate, heptanoate and 2-ethylhaxanoate. May act as an odorant transporter By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]
Sequence caution	The sequence BAD18487.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A6NK97-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: A6NK97-2)

The sequence of this isoform differs from the canonical sequence as follows:
     213-347: VEWMPTRGRT...RKVTCCLMVI → ASRVIPLAPP...KQILSSKFQS
     348-555: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Solute carrier family 22 member 20		PRO_0000326428
Regions
	Topological domain	1 – 23	23	Cytoplasmic Potential
	Transmembrane	24 – 44	21	Helical; Potential
	Topological domain	45 – 137	93	Extracellular Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Topological domain	159 – 166	8	Cytoplasmic Potential
	Transmembrane	167 – 187	21	Helical; Potential
	Topological domain	188 – 194	7	Extracellular Potential
	Transmembrane	195 – 215	21	Helical; Potential
	Topological domain	216 – 225	10	Cytoplasmic Potential
	Transmembrane	226 – 246	21	Helical; Potential
	Topological domain	247 – 250	4	Extracellular Potential
	Transmembrane	251 – 271	21	Helical; Potential
	Topological domain	272 – 339	68	Cytoplasmic Potential
	Transmembrane	340 – 360	21	Helical; Potential
	Topological domain	361 – 373	13	Extracellular Potential
	Transmembrane	374 – 394	21	Helical; Potential
	Topological domain	395 – 397	3	Cytoplasmic Potential
	Transmembrane	398 – 418	21	Helical; Potential
	Topological domain	419 – 429	11	Extracellular Potential
	Transmembrane	430 – 450	21	Helical; Potential
	Topological domain	451 – 485	35	Cytoplasmic Potential
	Transmembrane	486 – 506	21	Helical; Potential
	Topological domain	507 – 555	49	Extracellular Potential
	Compositional bias	23 – 26	4	Poly-Leu
Amino acid modifications
	Glycosylation	54	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	213 – 347	135	VEWMP…CLMVI → ASRVIPLAPPAWQVPVSCTE SAEGGCNEREEGGRGKADQG GDELLHPKRVCKCLHLQLNL GPLPNPGHPQGHMLSHGDLR GRQRSSGSGEQGGSCEPESR NGRLPAVAPAGLGLPFVHLP LSAREKQILSSKFQS in isoform 2.		VSP_032653
	Alternative sequence	348 – 555	208	Missing in isoform 2.		VSP_032654
	Natural variant	58	1	A → D. Corresponds to variant rs11605576 [ dbSNP | Ensembl ].		VAR_040065
	Natural variant	139	1	A → V. Corresponds to variant rs12420456 [ dbSNP | Ensembl ].		VAR_040066
Isoform 2:
	Natural variant	244	1	G → G. Corresponds to variant rs514076 [ dbSNP | Ensembl ].

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 3F3D75A7FFB5E91C	FASTA	555	60,459
	10 20 30 40 50 60 MAFTDLLDAL GSMGRFQLNH TALLLLPCGL LACHNFLQNF TAAVPPHHCR GPANHTEAST 70 80 90 100 110 120 NDSGAWLRAT IPLDQLGAPE PCRRFTKPQW ALLSPNSSIP GAATEGCKDG WVYNRSVFPS 130 140 150 160 170 180 TIVMEWDLVC EARTLRDLAQ SVYMAGVLVG AAVFGSLADR LGCKGPLVWS YLQLAASGAA 190 200 210 220 230 240 TAYFSSFSAY CVFRFLMGMT FSGIILNSVS LVVEWMPTRG RTVAGILLGY SFTLGQLILA 250 260 270 280 290 300 GVAYLIRPWR CLQFAISAPF LIFFLYSWWL PESSRWLLLH GKSQLAVQNL QKVAAMNGRK 310 320 330 340 350 360 EEGERLTKEV MSSYIQSEFA SVCTSNSILD LFRTPAIRKV TCCLMVIWFS NSVAYYGLAM 370 380 390 400 410 420 DLQKFGLSLY LVQALFGIIN TPAMLVATAT MIYVGRRATV ASFLILAGLM VIANMFVPEG 430 440 450 460 470 480 TQILCTAQAA LGKGCLASSF ICVYLFTGEL YPTEIRQMGM GFASVHARLG GLTAPLVTTL 490 500 510 520 530 540 GEYSTILPPV SFGATAILAG LAVCVLTETR NMPLVETIAA MERRVKEGSS KKHVEEKSEE 550 ISLQQLRASP LKETI
	Isoform 2 [UniParc]. Checksum: D03C6E98DCA8A298	FASTA	347	37,029
	10 20 30 40 50 60 MAFTDLLDAL GSMGRFQLNH TALLLLPCGL LACHNFLQNF TAAVPPHHCR GPANHTEAST 70 80 90 100 110 120 NDSGAWLRAT IPLDQLGAPE PCRRFTKPQW ALLSPNSSIP GAATEGCKDG WVYNRSVFPS 130 140 150 160 170 180 TIVMEWDLVC EARTLRDLAQ SVYMAGVLVG AAVFGSLADR LGCKGPLVWS YLQLAASGAA 190 200 210 220 230 240 TAYFSSFSAY CVFRFLMGMT FSGIILNSVS LVASRVIPLA PPAWQVPVSC TESAEGGCNE 250 260 270 280 290 300 REEGGRGKAD QGGDELLHPK RVCKCLHLQL NLGPLPNPGH PQGHMLSHGD LRGRQRSSGS 310 320 330 340 GEQGGSCEPE SRNGRLPAVA PAGLGLPFVH LPLSAREKQI LSSKFQS

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Synovium.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK131327 mRNA. Translation: BAD18487.1. Different initiation. AP003068 Genomic DNA. No translation available. BC146850 mRNA. Translation: AAI46851.1.
IPI	IPI00418585. IPI00783068.
RefSeq	NP_001004326.4. NM_001004326.4.
UniGene	Hs.532372.
3D structure databases
ProteinModelPortal	A6NK97.
ModBase	Search...
PTM databases
PhosphoSite	A6NK97.
Proteomic databases
PaxDb	A6NK97.
PRIDE	A6NK97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	440044.
KEGG	hsa:440044.
UCSC	uc021qlg.1. human. uc021qlh.1. human.
Organism-specific databases
CTD	440044.
GeneCards	GC11P064981.
HGNC	HGNC:29867. SLC22A20.
MIM	611696. gene.
neXtProt	NX_A6NK97.
PharmGKB	PA162403493.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000234569.
HOVERGEN	HBG108433.
KO	K08216.
OrthoDB	EOG418BPG.
Gene expression databases
CleanEx	HS_SLC22A20.
Genevestigator	A6NK97.
Family and domain databases
InterPro	IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR005828. Sub_transporter. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	440044.
NextBio	108866.
SOURCE	Search...

Entry information

Entry name

S22AK_HUMAN

Accession

Primary (citable) accession number: A6NK97
Secondary accession number(s): B9EJB2, Q6ZN88

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 24, 2007
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.