A6NJ78 (MET15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable methyltransferase-like protein 15
EC=2.1.1.-

Alternative name(s):
Methyltransferase 5 domain-containing protein 1

Gene names

Name:	METTL15
Synonyms:	METT5D1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable S-adenosyl-L-methionine-dependent methyltransferase By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.
Sequence caution	The sequence AAH30997.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A6NJ78-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A6NJ78-2)

The sequence of this isoform differs from the canonical sequence as follows:
     260-273: GAFPPSAIYTRKDL → EYSETYFPVAQTLY
     274-407: Missing.

Isoform 3 (identifier: A6NJ78-3)

The sequence of this isoform differs from the canonical sequence as follows:
     91-99: IFLDMTFGS → SLGVRSVGI
     100-407: Missing.

Isoform 4 (identifier: A6NJ78-4)

The sequence of this isoform differs from the canonical sequence as follows:
     201-320: YPDMPTAADV...RPGGRLVALS → STGTCIYPKN...FPGSSHICEQ
     321-407: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Probable methyltransferase-like protein 15		PRO_0000308332
Regions
	Region	100 – 102	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	119	1	S-adenosyl-L-methionine By similarity
	Binding site	146	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	169	1	S-adenosyl-L-methionine By similarity
	Binding site	176	1	S-adenosyl-L-methionine By similarity
Natural variations
	Alternative sequence	91 – 99	9	IFLDMTFGS → SLGVRSVGI in isoform 3.		VSP_028970
	Alternative sequence	100 – 407	308	Missing in isoform 3.		VSP_028971
	Alternative sequence	201 – 320	120	YPDMP…LVALS → STGTCIYPKNIRGGEACQEN RFSNCSGTQHLPHHQNPAAC QHRCRSISSLCYLYTERLTT AIYPYCHQDFPGSSHICEQ in isoform 4.		VSP_028972
	Alternative sequence	260 – 273	14	GAFPP…TRKDL → EYSETYFPVAQTLY in isoform 2.		VSP_028973
	Alternative sequence	274 – 407	134	Missing in isoform 2.		VSP_028974
	Alternative sequence	321 – 407	87	Missing in isoform 4.		VSP_028975
	Natural variant	31	1	N → K. Ref.1 Ref.4 Corresponds to variant rs2883478 [ dbSNP | Ensembl ].		VAR_036801
	Natural variant	149	1	A → T. Corresponds to variant rs11823114 [ dbSNP | Ensembl ].		VAR_036802
	Natural variant	267	1	I → F. Corresponds to variant rs11030280 [ dbSNP | Ensembl ].		VAR_059446
Experimental info
	Sequence conflict	191	1	P → S in AAH30997. Ref.4
	Sequence conflict	194	1	M → V in BAC03631. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 1DA02B5842483080	FASTA	407	46,121
	10 20 30 40 50 60 MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD QTQAQELHRS 70 80 90 100 110 120 QDRDFETMAK LHIPVMVDEV VHCLSPQKGQ IFLDMTFGSG GHTKAILQKE SDIVLYALDR 130 140 150 160 170 180 DPTAYALAEH LSELYPKQIR AMLGQFSQAE ALLMKAGVQP GTFDGVLMDL GCSSMQLDTP 190 200 210 220 230 240 ERGFSLRKDG PLDMRMDGGR YPDMPTAADV VNALDQQALA SILRTYGEEK HAKKIASAIV 250 260 270 280 290 300 QARSIYPITR TQQLASIVAG AFPPSAIYTR KDLLQRSTHI ATKTFQALRI FVNNELNELY 310 320 330 340 350 360 TGLKTAQKFL RPGGRLVALS FHSLEDRIVK RFLLGISMTE RFNLSVRQQV MKTSQLGSDH 370 380 390 400 ENTEEVSMRR APLMWELIHK KVLSPQDQDV QDNPRGRSAK LRAAIKL
	Isoform 2 [UniParc]. Checksum: 71BBA524769D6066	FASTA	273	30,872
	10 20 30 40 50 60 MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD QTQAQELHRS 70 80 90 100 110 120 QDRDFETMAK LHIPVMVDEV VHCLSPQKGQ IFLDMTFGSG GHTKAILQKE SDIVLYALDR 130 140 150 160 170 180 DPTAYALAEH LSELYPKQIR AMLGQFSQAE ALLMKAGVQP GTFDGVLMDL GCSSMQLDTP 190 200 210 220 230 240 ERGFSLRKDG PLDMRMDGGR YPDMPTAADV VNALDQQALA SILRTYGEEK HAKKIASAIV 250 260 270 QARSIYPITR TQQLASIVAE YSETYFPVAQ TLY
	Isoform 3 [UniParc]. Checksum: 0A6B26342AB079D8	FASTA	99	11,631
	10 20 30 40 50 60 MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD QTQAQELHRS 70 80 90 QDRDFETMAK LHIPVMVDEV VHCLSPQKGQ SLGVRSVGI
	Isoform 4 [UniParc]. Checksum: B698284087F05A6E	FASTA	279	31,700
	10 20 30 40 50 60 MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD QTQAQELHRS 70 80 90 100 110 120 QDRDFETMAK LHIPVMVDEV VHCLSPQKGQ IFLDMTFGSG GHTKAILQKE SDIVLYALDR 130 140 150 160 170 180 DPTAYALAEH LSELYPKQIR AMLGQFSQAE ALLMKAGVQP GTFDGVLMDL GCSSMQLDTP 190 200 210 220 230 240 ERGFSLRKDG PLDMRMDGGR STGTCIYPKN IRGGEACQEN RFSNCSGTQH LPHHQNPAAC 250 260 270 QHRCRSISSL CYLYTERLTT AIYPYCHQDF PGSSHICEQ

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT LYS-31.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4), VARIANT LYS-31. Tissue: Brain and Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK091298 mRNA. Translation: BAC03631.1. AC023206 Genomic DNA. No translation available. AC087376 Genomic DNA. No translation available. CH471064 Genomic DNA. Translation: EAW68267.1. CH471064 Genomic DNA. Translation: EAW68266.1. BC030997 mRNA. Translation: AAH30997.1. Different initiation. BC105287 mRNA. Translation: AAI05288.1.
IPI	IPI00384389. IPI00641281. IPI00654846. IPI00783001.
RefSeq	NP_001107000.1. NM_001113528.1. NP_689849.2. NM_152636.2.
UniGene	Hs.243326.
3D structure databases
ProteinModelPortal	A6NJ78.
SMR	A6NJ78. Positions 71-407.
ModBase	Search...
Protein-protein interaction databases
STRING	A6NJ78.
PTM databases
PhosphoSite	A6NJ78.
Proteomic databases
PRIDE	A6NJ78.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000407364; ENSP00000384369; ENSG00000169519.
GeneID	196074.
KEGG	hsa:196074.
UCSC	uc001mse.2. human. uc001msg.2. human. uc001msh.2. human.
Organism-specific databases
CTD	196074.
GeneCards	GC11P028129.
HGNC	HGNC:26606. METTL15.
neXtProt	NX_A6NJ78.
PharmGKB	PA142671458.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG16317.
HOGENOM	HBG302779.
HOVERGEN	HBG055013.
InParanoid	A6NJ78.
OMA	NPATRAF.
PhylomeDB	A6NJ78.
Gene expression databases
Bgee	A6NJ78.
CleanEx	HS_METT5D1.
Genevestigator	A6NJ78.
Family and domain databases
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...
Other
NextBio	89414.

Entry information

Entry name

MET15_HUMAN

Accession

Primary (citable) accession number: A6NJ78
Secondary accession number(s): A8MRS5 , B7WNU2, Q3MHD3, Q8N601, Q8NBA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	July 24, 2007
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.