##gff-version 3
A6NI61	UniProtKB	Chain	1	221	.	.	.	ID=PRO_0000319062;Note=Protein myomaker	
A6NI61	UniProtKB	Topological domain	1	3	.	.	.	Note=Extracellular;Ontology_term=ECO:0000305;evidence=ECO:0000305	
A6NI61	UniProtKB	Transmembrane	4	24	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
A6NI61	UniProtKB	Topological domain	25	34	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305	
A6NI61	UniProtKB	Transmembrane	35	55	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
A6NI61	UniProtKB	Topological domain	56	64	.	.	.	Note=Extracellular;Ontology_term=ECO:0000305;evidence=ECO:0000305	
A6NI61	UniProtKB	Transmembrane	65	85	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
A6NI61	UniProtKB	Topological domain	86	92	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305	
A6NI61	UniProtKB	Transmembrane	93	110	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
A6NI61	UniProtKB	Topological domain	111	113	.	.	.	Note=Extracellular;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9D1N4	
A6NI61	UniProtKB	Transmembrane	114	134	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
A6NI61	UniProtKB	Topological domain	135	153	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9D1N4	
A6NI61	UniProtKB	Transmembrane	154	174	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
A6NI61	UniProtKB	Topological domain	175	175	.	.	.	Note=Extracellular;Ontology_term=ECO:0000305;evidence=ECO:0000305	
A6NI61	UniProtKB	Transmembrane	176	196	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
A6NI61	UniProtKB	Topological domain	197	221	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305	
A6NI61	UniProtKB	Lipidation	217	217	.	.	.	Note=S-palmitoyl cysteine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9D1N4	
A6NI61	UniProtKB	Lipidation	218	218	.	.	.	Note=S-palmitoyl cysteine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9D1N4	
A6NI61	UniProtKB	Natural variant	79	79	.	.	.	ID=VAR_081291;Note=In CFZS1%3B uncertain significance. W->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30065953;Dbxref=PMID:30065953	
A6NI61	UniProtKB	Natural variant	91	91	.	.	.	ID=VAR_079262;Note=In CFZS1%3B uncertain significance%3B supports cell fusion in a heterologous cell fusion assay in vitro%3B decreased localization at the plasma membrane%3B partially rescues of mymk knockout fish%3B primary myoblasts from a compound heterozygote associating T-91 and R-185 exhibit no difference in their capability to differentiate compared to control myoblasts%2C but show a significant difference in the fusion index%2C with a higher percentage of singly-nucleated relative to multinucleated cells in compound heterozygous compared to control myoblasts. P->T;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:28681861,ECO:0000269|PubMed:30065953;Dbxref=dbSNP:rs137868995,PMID:28681861,PMID:30065953	
A6NI61	UniProtKB	Natural variant	100	100	.	.	.	ID=VAR_079263;Note=In CFZS1%3B loss of localization at the plasma membrane%3B loss of cell fusion in a heterologous cell fusion assay in vitro%3B no rescue of mymk knockout fish. G->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28681861;Dbxref=dbSNP:rs964335184,PMID:28681861	
A6NI61	UniProtKB	Natural variant	154	154	.	.	.	ID=VAR_079264;Note=In CFZS1%3B decreased localization at the plasma membrane%3B reduced cell fusion in a heterologous cell fusion assay in vitro compared to wild-type%3B partially rescues of mymk knockout fish. I->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28681861;Dbxref=dbSNP:rs1131692249,PMID:28681861	
A6NI61	UniProtKB	Natural variant	185	185	.	.	.	ID=VAR_079265;Note=In CFZS1%3B loss of localization at the plasma membrane%3B loss of cell fusion in a heterologous cell fusion assay in vitro%3B no rescue of mymk knockout fish%3B primary myoblasts from a compound heterozygote associating T-91 and R-185 exhibit no difference in their capability to differentiate compared to control myoblasts%2C but show a significant difference in the fusion index%2C with a higher percentage of singly-nucleated relative to multinucleated cells in compound heterozygous compared to control myoblasts. C->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28681861;Dbxref=dbSNP:rs1131692247,PMID:28681861