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A6NHC0

- CAN8_HUMAN

UniProt

A6NHC0 - CAN8_HUMAN

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Protein

Calpain-8

Gene

CAPN8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex (By similarity).By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 2 calcium ions.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei105 – 1051By similarity
Active sitei262 – 2621By similarity
Active sitei286 – 2861By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi588 – 599121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi618 – 629122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: RefGenome
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. digestion Source: InterPro
  2. proteolysis Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-8 (EC:3.4.22.53)
Alternative name(s):
New calpain 2
Short name:
nCL-2
Stomach-specific M-type calpain
Gene namesi
Name:CAPN8
Synonyms:NCL2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:1485. CAPN8.

Subcellular locationi

Cytoplasm By similarity. Golgi apparatus By similarity

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. Golgi apparatus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 703703Calpain-8PRO_0000349280Add
BLAST

Post-translational modificationi

Undergoes autolytic cleavage between Ala-5 and Ala-6 which gives rise to fragments extending from Ala-6 to the C-terminus, Ala-6 to the EF-hand 2 domain and from Ala-6 to the beginning of domain III.By similarity

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PaxDbiA6NHC0.
PRIDEiA6NHC0.

PTM databases

PhosphoSiteiA6NHC0.

Expressioni

Tissue specificityi

Stomach.

Gene expression databases

BgeeiA6NHC0.
CleanExiHS_CAPN8.
ExpressionAtlasiA6NHC0. baseline and differential.
GenevestigatoriA6NHC0.

Organism-specific databases

HPAiHPA021480.

Interactioni

Subunit structurei

Monomer and homooligomer. Interacts with COPS1/GPS1, COPB1, EYA2, NME2, NME4 and TOMM70A (By similarity).By similarity

Protein-protein interaction databases

STRINGi9606.ENSP00000397140.

Structurei

3D structure databases

ProteinModelPortaliA6NHC0.
SMRiA6NHC0. Positions 2-699.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini575 – 61036EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini605 – 64036EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini670 – 70334EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni356 – 37924Domain IIIAdd
BLAST

Domaini

The domain III mediates oligomerization.By similarity

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiA6NHC0.
KOiK08577.
OrthoDBiEOG7RV9FM.
PhylomeDBiA6NHC0.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029543. CAPN8.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF52. PTHR10183:SF52. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6NHC0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAQAAGVSR QRAATQGLGS NQNALKYLGQ DFKTLRQQCL DSGVLFKDPE
60 70 80 90 100
FPACPSALGY KDLGPGSPQT QGIIWKRPTE LCPSPQFIVG GATRTDICQG
110 120 130 140 150
GLGDCWLLAA IASLTLNEEL LYRVVPRDQD FQENYAGIFH FQFWQYGEWV
160 170 180 190 200
EVVIDDRLPT KNGQLLFLHS EQGNEFWSAL LEKAYAKLNG CYEALAGGST
210 220 230 240 250
VEGFEDFTGG ISEFYDLKKP PANLYQIIRK ALCAGSLLGC SIDVSSAAEA
260 270 280 290 300
EAITSQKLVK SHAYSVTGVE EVNFQGHPEK LIRLRNPWGE VEWSGAWSDD
310 320 330 340 350
APEWNHIDPR RKEELDKKVE DGEFWMSLSD FVRQFSRLEI CNLSPDSLSS
360 370 380 390 400
EEVHKWNLVL FNGHWTRGST AGGCQNYPAT YWTNPQFKIR LDEVDEDQEE
410 420 430 440 450
SIGEPCCTVL LGLMQKNRRW RKRIGQGMLS IGYAVYQVPK ELESHTDAHL
460 470 480 490 500
GRDFFLAYQP SARTSTYVNL REVSGRARLP PGEYLVVPST FEPFKDGEFC
510 520 530 540 550
LRVFSEKKAQ ALEIGDVVAG NPYEPHPSEV DQEDDQFRRL FEKLAGKDSE
560 570 580 590 600
ITANALKILL NEAFSKRTDI KFDGFNINTC REMISLLDSN GTGTLGAVEF
610 620 630 640 650
KTLWLKIQKY LEIYWETDYN HSGTIDAHEM RTALRKAGFT LNSQVQQTIA
660 670 680 690 700
LRYACSKLGI NFDSFVACMI RLETLFKLFS LLDEDKDGMV QLSLAEWLCC

VLV
Length:703
Mass (Da):79,144
Last modified:August 10, 2010 - v3
Checksum:i2A6DFFC0814894A8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti245 – 2451S → Y in AAI57894. (PubMed:15489334)Curated
Sequence conflicti592 – 5921T → M in AAI57894. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC099065 Genomic DNA. No translation available.
CR628412 Genomic DNA. No translation available.
CR848842 Genomic DNA. No translation available.
BC157893 mRNA. Translation: AAI57894.1.
CCDSiCCDS73038.1.
RefSeqiNP_001137434.1. NM_001143962.1.
UniGeneiHs.291487.

Genome annotation databases

EnsembliENST00000366872; ENSP00000355837; ENSG00000203697.
GeneIDi388743.
KEGGihsa:388743.
UCSCiuc009xee.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC099065 Genomic DNA. No translation available.
CR628412 Genomic DNA. No translation available.
CR848842 Genomic DNA. No translation available.
BC157893 mRNA. Translation: AAI57894.1 .
CCDSi CCDS73038.1.
RefSeqi NP_001137434.1. NM_001143962.1.
UniGenei Hs.291487.

3D structure databases

ProteinModelPortali A6NHC0.
SMRi A6NHC0. Positions 2-699.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000397140.

Protein family/group databases

MEROPSi C02.007.

PTM databases

PhosphoSitei A6NHC0.

Proteomic databases

PaxDbi A6NHC0.
PRIDEi A6NHC0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000366872 ; ENSP00000355837 ; ENSG00000203697 .
GeneIDi 388743.
KEGGi hsa:388743.
UCSCi uc009xee.2. human.

Organism-specific databases

CTDi 388743.
GeneCardsi GC01M223711.
HGNCi HGNC:1485. CAPN8.
HPAi HPA021480.
neXtProti NX_A6NHC0.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG327523.
GeneTreei ENSGT00760000118971.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi A6NHC0.
KOi K08577.
OrthoDBi EOG7RV9FM.
PhylomeDBi A6NHC0.
TreeFami TF314748.

Miscellaneous databases

GenomeRNAii 388743.
NextBioi 102335.
PROi A6NHC0.

Gene expression databases

Bgeei A6NHC0.
CleanExi HS_CAPN8.
ExpressionAtlasi A6NHC0. baseline and differential.
Genevestigatori A6NHC0.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029543. CAPN8.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF52. PTHR10183:SF52. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Both the conserved and the unique gene structure of stomach-specific calpains reveal processes of calpain gene evolution."
    Hata S., Nishi K., Kawamoto T., Lee H.-J., Kawahara H., Maeda T., Shintani Y., Sorimachi H., Suzuki K.
    J. Mol. Evol. 53:191-203(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leukocyte.
  2. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiCAN8_HUMAN
AccessioniPrimary (citable) accession number: A6NHC0
Secondary accession number(s): B2RXL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: August 10, 2010
Last modified: October 29, 2014
This is version 66 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3