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A6NHC0

- CAN8_HUMAN

UniProt

A6NHC0 - CAN8_HUMAN

Protein

Calpain-8

Gene

CAPN8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 3 (10 Aug 2010)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex By similarity.By similarity

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 2 calcium ions.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei105 – 1051By similarity
    Active sitei262 – 2621By similarity
    Active sitei286 – 2861By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi588 – 599121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi618 – 629122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: RefGenome
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiC02.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-8 (EC:3.4.22.53)
    Alternative name(s):
    New calpain 2
    Short name:
    nCL-2
    Stomach-specific M-type calpain
    Gene namesi
    Name:CAPN8
    Synonyms:NCL2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:1485. CAPN8.

    Subcellular locationi

    Cytoplasm By similarity. Golgi apparatus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. Golgi apparatus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 703703Calpain-8PRO_0000349280Add
    BLAST

    Post-translational modificationi

    Undergoes autolytic cleavage between Ala-5 and Ala-6 which gives rise to fragments extending from Ala-6 to the C-terminus, Ala-6 to the EF-hand 2 domain and from Ala-6 to the beginning of domain III.By similarity

    Keywords - PTMi

    Autocatalytic cleavage

    Proteomic databases

    PaxDbiA6NHC0.
    PRIDEiA6NHC0.

    PTM databases

    PhosphoSiteiA6NHC0.

    Expressioni

    Tissue specificityi

    Stomach.

    Gene expression databases

    ArrayExpressiA6NHC0.
    BgeeiA6NHC0.
    CleanExiHS_CAPN8.
    GenevestigatoriA6NHC0.

    Organism-specific databases

    HPAiHPA021480.

    Interactioni

    Subunit structurei

    Monomer and homooligomer. Interacts with COPS1/GPS1, COPB1, EYA2, NME2, NME4 and TOMM70A By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9606.ENSP00000397140.

    Structurei

    3D structure databases

    ProteinModelPortaliA6NHC0.
    SMRiA6NHC0. Positions 2-699.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini575 – 61036EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini605 – 64036EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini670 – 70334EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni356 – 37924Domain IIIAdd
    BLAST

    Domaini

    The domain III mediates oligomerization.By similarity

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG327523.
    HOGENOMiHOG000232035.
    HOVERGENiHBG012645.
    InParanoidiA6NHC0.
    KOiK08577.
    OrthoDBiEOG7RV9FM.
    PhylomeDBiA6NHC0.
    TreeFamiTF314748.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029543. CAPN8.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PANTHERiPTHR10183:SF52. PTHR10183:SF52. 1 hit.
    PfamiPF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6NHC0-1 [UniParc]FASTAAdd to Basket

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    MAAQAAGVSR QRAATQGLGS NQNALKYLGQ DFKTLRQQCL DSGVLFKDPE    50
    FPACPSALGY KDLGPGSPQT QGIIWKRPTE LCPSPQFIVG GATRTDICQG 100
    GLGDCWLLAA IASLTLNEEL LYRVVPRDQD FQENYAGIFH FQFWQYGEWV 150
    EVVIDDRLPT KNGQLLFLHS EQGNEFWSAL LEKAYAKLNG CYEALAGGST 200
    VEGFEDFTGG ISEFYDLKKP PANLYQIIRK ALCAGSLLGC SIDVSSAAEA 250
    EAITSQKLVK SHAYSVTGVE EVNFQGHPEK LIRLRNPWGE VEWSGAWSDD 300
    APEWNHIDPR RKEELDKKVE DGEFWMSLSD FVRQFSRLEI CNLSPDSLSS 350
    EEVHKWNLVL FNGHWTRGST AGGCQNYPAT YWTNPQFKIR LDEVDEDQEE 400
    SIGEPCCTVL LGLMQKNRRW RKRIGQGMLS IGYAVYQVPK ELESHTDAHL 450
    GRDFFLAYQP SARTSTYVNL REVSGRARLP PGEYLVVPST FEPFKDGEFC 500
    LRVFSEKKAQ ALEIGDVVAG NPYEPHPSEV DQEDDQFRRL FEKLAGKDSE 550
    ITANALKILL NEAFSKRTDI KFDGFNINTC REMISLLDSN GTGTLGAVEF 600
    KTLWLKIQKY LEIYWETDYN HSGTIDAHEM RTALRKAGFT LNSQVQQTIA 650
    LRYACSKLGI NFDSFVACMI RLETLFKLFS LLDEDKDGMV QLSLAEWLCC 700
    VLV 703
    Length:703
    Mass (Da):79,144
    Last modified:August 10, 2010 - v3
    Checksum:i2A6DFFC0814894A8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti245 – 2451S → Y in AAI57894. (PubMed:15489334)Curated
    Sequence conflicti592 – 5921T → M in AAI57894. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC099065 Genomic DNA. No translation available.
    CR628412 Genomic DNA. No translation available.
    CR848842 Genomic DNA. No translation available.
    BC157893 mRNA. Translation: AAI57894.1.
    RefSeqiNP_001137434.1. NM_001143962.1.
    UniGeneiHs.291487.

    Genome annotation databases

    EnsembliENST00000366872; ENSP00000355837; ENSG00000203697.
    GeneIDi388743.
    KEGGihsa:388743.
    UCSCiuc009xee.2. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC099065 Genomic DNA. No translation available.
    CR628412 Genomic DNA. No translation available.
    CR848842 Genomic DNA. No translation available.
    BC157893 mRNA. Translation: AAI57894.1 .
    RefSeqi NP_001137434.1. NM_001143962.1.
    UniGenei Hs.291487.

    3D structure databases

    ProteinModelPortali A6NHC0.
    SMRi A6NHC0. Positions 2-699.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000397140.

    Protein family/group databases

    MEROPSi C02.007.

    PTM databases

    PhosphoSitei A6NHC0.

    Proteomic databases

    PaxDbi A6NHC0.
    PRIDEi A6NHC0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000366872 ; ENSP00000355837 ; ENSG00000203697 .
    GeneIDi 388743.
    KEGGi hsa:388743.
    UCSCi uc009xee.2. human.

    Organism-specific databases

    CTDi 388743.
    GeneCardsi GC01M223711.
    HGNCi HGNC:1485. CAPN8.
    HPAi HPA021480.
    neXtProti NX_A6NHC0.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG327523.
    HOGENOMi HOG000232035.
    HOVERGENi HBG012645.
    InParanoidi A6NHC0.
    KOi K08577.
    OrthoDBi EOG7RV9FM.
    PhylomeDBi A6NHC0.
    TreeFami TF314748.

    Miscellaneous databases

    GenomeRNAii 388743.
    NextBioi 102335.
    PROi A6NHC0.

    Gene expression databases

    ArrayExpressi A6NHC0.
    Bgeei A6NHC0.
    CleanExi HS_CAPN8.
    Genevestigatori A6NHC0.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029543. CAPN8.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    PANTHERi PTHR10183:SF52. PTHR10183:SF52. 1 hit.
    Pfami PF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Both the conserved and the unique gene structure of stomach-specific calpains reveal processes of calpain gene evolution."
      Hata S., Nishi K., Kawamoto T., Lee H.-J., Kawahara H., Maeda T., Shintani Y., Sorimachi H., Suzuki K.
      J. Mol. Evol. 53:191-203(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Leukocyte.
    2. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiCAN8_HUMAN
    AccessioniPrimary (citable) accession number: A6NHC0
    Secondary accession number(s): B2RXL2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: August 10, 2010
    Last modified: October 1, 2014
    This is version 65 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3