ID   URAD_HUMAN              Reviewed;         173 AA.
AC   A6NGE7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 2.
DT   24-JAN-2024, entry version 107.
DE   RecName: Full=Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase;
DE            Short=OHCU decarboxylase;
DE            EC=4.1.1.97;
DE   AltName: Full=Parahox neighbor;
DE   AltName: Full=Ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5-) decarboxylase;
GN   Name=URAD; Synonyms=PRHOXNB;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057823; DOI=10.1038/nature02379;
RA   Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA   Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S.,
RA   Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P.,
RA   Ambrose K.D., Andrews D.T., Ashwell R.I.S., Babbage A.K., Bagguley C.L.,
RA   Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P.,
RA   Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA   Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C.,
RA   Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P.,
RA   Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L.,
RA   Frankish A.G., Frankland J., French L., Garner P., Garnett J.,
RA   Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M.,
RA   Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D.,
RA   Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D.,
RA   Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J.,
RA   Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA   Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S.,
RA   Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R.,
RA   Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W.,
RA   Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P.,
RA   Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L.,
RA   Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R.,
RA   Rogers J., Ross M.T.;
RT   "The DNA sequence and analysis of human chromosome 13.";
RL   Nature 428:522-528(2004).
RN   [2]
RP   LACK OF TISSUE SPECIFICITY.
RX   PubMed=16462750; DOI=10.1038/nchembio768;
RA   Ramazzina I., Folli C., Secchi A., Berni R., Percudani R.;
RT   "Completing the uric acid degradation pathway through phylogenetic
RT   comparison of whole genomes.";
RL   Nat. Chem. Biol. 2:144-148(2006).
CC   -!- FUNCTION: Catalyzes the stereoselective decarboxylation of 2-oxo-4-
CC       hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin.
CC       {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-
CC         carboxylate + H(+) = (S)-allantoin + CO2; Xref=Rhea:RHEA:26301,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15678, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:58639; EC=4.1.1.97;
CC   -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC       urate: step 3/3.
CC   -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Apparently not expressed.
CC   -!- SIMILARITY: Belongs to the OHCU decarboxylase family. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. In primates the genes
CC       coding for the enzymes for the degradation of uric acid were
CC       inactivated and converted to pseudogenes (PubMed:16462750).
CC       {ECO:0000305|PubMed:16462750}.
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DR   EMBL; AL591024; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS45020.1; -.
DR   RefSeq; NP_001099047.1; NM_001105577.1.
DR   AlphaFoldDB; A6NGE7; -.
DR   SMR; A6NGE7; -.
DR   BioGRID; 571472; 1.
DR   IntAct; A6NGE7; 1.
DR   STRING; 9606.ENSP00000333490; -.
DR   iPTMnet; A6NGE7; -.
DR   PhosphoSitePlus; A6NGE7; -.
DR   BioMuta; URAD; -.
DR   MassIVE; A6NGE7; -.
DR   PaxDb; 9606-ENSP00000333490; -.
DR   Antibodypedia; 48958; 9 antibodies from 7 providers.
DR   DNASU; 646625; -.
DR   Ensembl; ENST00000332715.6; ENSP00000333490.4; ENSG00000183463.6.
DR   GeneID; 646625; -.
DR   KEGG; hsa:646625; -.
DR   MANE-Select; ENST00000332715.6; ENSP00000333490.4; NM_001105577.2; NP_001099047.1.
DR   UCSC; uc010aan.2; human.
DR   AGR; HGNC:17785; -.
DR   CTD; 646625; -.
DR   GeneCards; URAD; -.
DR   HGNC; HGNC:17785; URAD.
DR   HPA; ENSG00000183463; Tissue enriched (intestine).
DR   MIM; 615804; gene.
DR   neXtProt; NX_A6NGE7; -.
DR   PharmGKB; PA142671135; -.
DR   VEuPathDB; HostDB:ENSG00000183463; -.
DR   eggNOG; KOG0848; Eukaryota.
DR   GeneTree; ENSGT00940000153229; -.
DR   HOGENOM; CLU_092522_1_1_1; -.
DR   InParanoid; A6NGE7; -.
DR   OMA; RCQNERA; -.
DR   OrthoDB; 2903165at2759; -.
DR   PhylomeDB; A6NGE7; -.
DR   TreeFam; TF323276; -.
DR   PathwayCommons; A6NGE7; -.
DR   SignaLink; A6NGE7; -.
DR   UniPathway; UPA00394; UER00652.
DR   BioGRID-ORCS; 646625; 12 hits in 1087 CRISPR screens.
DR   GenomeRNAi; 646625; -.
DR   Pharos; A6NGE7; Tdark.
DR   PRO; PR:A6NGE7; -.
DR   Proteomes; UP000005640; Chromosome 13.
DR   RNAct; A6NGE7; Protein.
DR   Bgee; ENSG00000183463; Expressed in mucosa of transverse colon and 28 other cell types or tissues.
DR   GO; GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
DR   GO; GO:0016831; F:carboxy-lyase activity; ISS:UniProtKB.
DR   GO; GO:0000255; P:allantoin metabolic process; IEA:InterPro.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3330.10; Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; 1.
DR   InterPro; IPR018020; OHCU_decarboxylase.
DR   InterPro; IPR017580; OHCU_decarboxylase-1.
DR   InterPro; IPR036778; OHCU_decarboxylase_sf.
DR   NCBIfam; TIGR03164; UHCUDC; 1.
DR   PANTHER; PTHR43466; 2-OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE-RELATED; 1.
DR   PANTHER; PTHR43466:SF1; 2-OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE-RELATED; 1.
DR   Pfam; PF09349; OHCU_decarbox; 1.
DR   SUPFAM; SSF158694; UraD-Like; 1.
PE   5: Uncertain;
KW   Decarboxylase; Lyase; Peroxisome; Purine metabolism; Reference proteome.
FT   CHAIN           1..173
FT                   /note="Putative 2-oxo-4-hydroxy-4-carboxy-5-
FT                   ureidoimidazoline decarboxylase"
FT                   /id="PRO_0000315240"
FT   MOTIF           171..173
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        67
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255"
FT   BINDING         68
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         84..88
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         119..123
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   VARIANT         57
FT                   /note="Q -> P (in dbSNP:rs3897926)"
FT                   /id="VAR_053987"
SQ   SEQUENCE   173 AA;  19130 MW;  521C44BEF163077C CRC64;
     MDIEKVNSMD LGEFVDVFGN ATERCPLIAA AVWSQRPFSD LEDLEKHFFA FIDALAQSGQ
     EGILRCHPDL AGSELQRGTL TAESQREQSG AGLRSLGADE RLRLAELNAQ YRARFGFPFV
     LAARFSDRTA VPRELARRLL CPSAQELRTA LGEVKKIGSL RLADLLRADP AKL
//