A6NDV4 (TMM8B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane protein 8B

Alternative name(s):
Nasopharyngeal carcinoma-associated gene 6 protein
Protein NAG-5
Protein NGX6

Gene names

Name:	TMEM8B
Synonyms:	C9orf127, NGX6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a regulator of the EGFR pathway. Probable tumor suppressor which may function in cell growth, proliferation and adhesion. Ref.7 Ref.8 Ref.9 Ref.10
Subunit structure	Isoform 2 (via its cytoplasmic part) interacts with EZR. Ref.7 Ref.10
Subcellular location	Isoform 2: Cell membrane; Multi-pass membrane protein Probable. Cytoplasm Probable. Nucleus Probable. Mitochondrion. Endoplasmic reticulum. Note: Also detected in mitochondrion and endoplasmic reticulum. Ref.7 Ref.9
Induction	Isoform 2 is down-regulated in nasopharyngeal carcinoma cells. Ref.7
Post-translational modification	Isoform 2 is N-glycosylated. Ref.7
Sequence similarities	Belongs to the TMEM8 family. Contains 1 EGF-like domain.
Sequence caution	The sequence AAC28644.1 differs from that shown. Reason: Erroneous initiation. The sequence AAF34820.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAF34820.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part.

Ontologies

Keywords
Biological process	Cell adhesion Growth regulation
Cellular component	Cell membrane Cytoplasm Endoplasmic reticulum Membrane Mitochondrion Nucleus
Coding sequence diversity	Alternative splicing
Domain	EGF-like domain Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-matrix adhesion Inferred from mutant phenotype Ref.7. Source: HGNC regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW regulation of mitotic cell cycle Traceable author statement Ref.7. Source: HGNC
Cellular_component	cell surface Inferred from direct assay Ref.7. Source: HGNC endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay Ref.7. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A6NDV4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A6NDV4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     274-337: FYHACDQPGI...GAMLLSMALQ → VCGGVCILSL...GHFCKTHWSK
     338-472: Missing.

Isoform 3 (identifier: A6NDV4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.
     82-93: GGVLSLELQLNA → MWRPHFHTCPPQ
     274-337: FYHACDQPGI...GAMLLSMALQ → VCGGVCILSL...GHFCKTHWSK
     338-472: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Transmembrane protein 8B		PRO_0000333039
Regions
	Topological domain	1 – 233	233	Extracellular Potential
	Transmembrane	234 – 254	21	Helical; Potential
	Topological domain	255 – 257	3	Cytoplasmic Potential
	Transmembrane	258 – 277	20	Helical; Potential
	Topological domain	278 – 292	15	Extracellular Potential
	Transmembrane	293 – 313	21	Helical; Potential
	Topological domain	314 – 315	2	Cytoplasmic Potential
	Transmembrane	316 – 336	21	Helical; Potential
	Topological domain	337 – 342	6	Extracellular Potential
	Transmembrane	343 – 363	21	Helical; Potential
	Topological domain	364 – 379	16	Cytoplasmic Potential
	Transmembrane	380 – 400	21	Helical; Potential
	Topological domain	401 – 405	5	Extracellular Potential
	Transmembrane	406 – 426	21	Helical; Potential
	Topological domain	427 – 472	46	Cytoplasmic Potential
	Domain	182 – 221	40	EGF-like
Amino acid modifications
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	100	1	N-linked (GlcNAc...) Potential
	Disulfide bond	186 ↔ 196		By similarity
	Disulfide bond	190 ↔ 209		By similarity
	Disulfide bond	211 ↔ 220		By similarity
Natural variations
	Alternative sequence	1 – 81	81	Missing in isoform 3.		VSP_033450
	Alternative sequence	82 – 93	12	GGVLS…LQLNA → MWRPHFHTCPPQ in isoform 3.		VSP_033451
	Alternative sequence	274 – 337	64	FYHAC…SMALQ → VCGGVCILSLGACAWWWVTV CISTTFSEGLGMSVPSLCLL QTETAVLPKLSCIDNGHFCK THWSK in isoform 2 and isoform 3.		VSP_033452
	Alternative sequence	338 – 472	135	Missing in isoform 2 and isoform 3.		VSP_033453
Experimental info
	Sequence conflict	178	1	R → W in AAH98296. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 3E5EA4495CF54389	FASTA	472	51,941
	10 20 30 40 50 60 MNMPQSLGNQ PLPPEPPSLG TPAEGPGTTS PPEHCWPVRP TLRNELDTFS VHFYIFFGPS 70 80 90 100 110 120 VALPPERPAV FAMRLLPVLD SGGVLSLELQ LNASSVRQEN VTVFGCLTHE VPLSLGDAAV 130 140 150 160 170 180 TCSKESLAGF LLSVSATTRV ARLRIPFPQT GTWFLALRSL CGVGPRFVRC RNATAEVRMR 190 200 210 220 230 240 TFLSPCVDDC GPYGQCKLLR THNYLYAACE CKAGWRGWGC TDSADALTYG FQLLSTLLLC 250 260 270 280 290 300 LSNLMFLPPV VLAIRSRYVL EAAVYTFTMF FSTFYHACDQ PGIVVFCIMD YDVLQFCDFL 310 320 330 340 350 360 GSLMSVWVTV IAMARLQPVV KQVLYLLGAM LLSMALQLDR HGLWNLLGPS LFALGILATA 370 380 390 400 410 420 WTVRSVRRRH CYPPTWRRWL FYLCPGSLIA GSAVLLYAFV ETRDNYFYIH SIWHMLIAGS 430 440 450 460 470 VGFLLPPRAK TDHGVPSGAR ARGCGYQLCI NEQEELGLVG PGGATVSSIC AS
	Isoform 2 [UniParc]. Checksum: BBB601E2F7ADAF3E	FASTA	338	36,844
	10 20 30 40 50 60 MNMPQSLGNQ PLPPEPPSLG TPAEGPGTTS PPEHCWPVRP TLRNELDTFS VHFYIFFGPS 70 80 90 100 110 120 VALPPERPAV FAMRLLPVLD SGGVLSLELQ LNASSVRQEN VTVFGCLTHE VPLSLGDAAV 130 140 150 160 170 180 TCSKESLAGF LLSVSATTRV ARLRIPFPQT GTWFLALRSL CGVGPRFVRC RNATAEVRMR 190 200 210 220 230 240 TFLSPCVDDC GPYGQCKLLR THNYLYAACE CKAGWRGWGC TDSADALTYG FQLLSTLLLC 250 260 270 280 290 300 LSNLMFLPPV VLAIRSRYVL EAAVYTFTMF FSTVCGGVCI LSLGACAWWW VTVCISTTFS 310 320 330 EGLGMSVPSL CLLQTETAVL PKLSCIDNGH FCKTHWSK
	Isoform 3 [UniParc]. Checksum: A482F1C8662472A8	FASTA	257	28,336
	10 20 30 40 50 60 MWRPHFHTCP PQSSVRQENV TVFGCLTHEV PLSLGDAAVT CSKESLAGFL LSVSATTRVA 70 80 90 100 110 120 RLRIPFPQTG TWFLALRSLC GVGPRFVRCR NATAEVRMRT FLSPCVDDCG PYGQCKLLRT 130 140 150 160 170 180 HNYLYAACEC KAGWRGWGCT DSADALTYGF QLLSTLLLCL SNLMFLPPVV LAIRSRYVLE 190 200 210 220 230 240 AAVYTFTMFF STVCGGVCIL SLGACAWWWV TVCISTTFSE GLGMSVPSLC LLQTETAVLP 250 KLSCIDNGHF CKTHWSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proteomic detection of changes in protein synthesis induced by NGX6 transfected in human nasopharyngeal carcinoma cells." Li J., Tan C., Xiang Q., Zhang X., Ma J., Wang J.-R., Yang J., Li W.-F., Shen S.-R., Liang S., Li G.-Y. J. Protein Chem. 20:265-271(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	Yu W., Gibbs R.A. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Brain.
[7]	"Role of a novel EGF-like domain-containing gene NGX6 in cell adhesion modulation in nasopharyngeal carcinoma cells." Ma J., Zhou J., Fan S., Wang L.-L., Li X.-L., Yan Q., Zhou M., Liu H.-Y., Zhang Q., Zhou H., Gan K., Li Z., Peng C., Xiong W., Tan C., Shen S.-R., Yang J., Li J., Li G.-Y. Carcinogenesis 26:281-291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH EZR, SUBCELLULAR LOCATION, GLYCOSYLATION, INDUCTION.
[8]	"NGX6 gene inhibits cell proliferation and plays a negative role in EGFR pathway in nasopharyngeal carcinoma cells." Wang L.-L., Ma J., Li J., Li X.-L., Zhang Q., Peng S.-P., Peng C., Zhou M., Xiong W., Yang J., Zhou J., Fan S., Tan C., Yan Q., Shen S.-R., Li G.-Y. J. Cell. Biochem. 95:64-73(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"The role of NGX6 and its deletion mutants in the proliferation, adhesion and migration of nasopharyngeal carcinoma 5-8F cells." Peng S.P., Li X.-L., Wang L., Ou-Yang J., Ma J., Wang L.-L., Liu H.-Y., Zhou M., Tang Y.-L., Li W.-S., Luo X.-M., Cao L., Tang K., Shen S.-R., Li G.-Y. Oncology 71:273-281(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]	"The expression of ezrin in NPC and its interaction with NGX6, a novel candidate suppressor." Peng S.-P., Fan S., Li X.-L., Wang L., Liu H.-Y., Zhou M., Wang L.-L., Shen S.-R., Li G.-Y. Cancer Sci. 98:341-349(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH EZR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF149297 mRNA. Translation: AAF34820.1. Sequence problems. AF188239 mRNA. Translation: AAG29055.1. AF070572 mRNA. Translation: AAC28644.1. Different initiation. AK074844 mRNA. Translation: BAG52015.1. AL133410 Genomic DNA. Translation: CAI10995.1. AL133410 Genomic DNA. Translation: CAI10996.2. CH471071 Genomic DNA. Translation: EAW58325.1. BC041377 mRNA. No translation available. BC098265 mRNA. Translation: AAH98265.1. BC098296 mRNA. Translation: AAH98296.1. BC099733 mRNA. Translation: AAH99733.1.
IPI	IPI00415061. IPI00513780. IPI00894263.
RefSeq	NP_001036054.1. NM_001042589.2. NP_001036055.1. NM_001042590.2. NP_057530.2. NM_016446.3.
UniGene	Hs.493808.
3D structure databases
ProteinModelPortal	A6NDV4.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-2873602.
Proteomic databases
PaxDb	A6NDV4.
PRIDE	A6NDV4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000377988; ENSP00000367227; ENSG00000137103. ENST00000377991; ENSP00000367230; ENSG00000137103. ENST00000377996; ENSP00000367235; ENSG00000137103. ENST00000439587; ENSP00000395810; ENSG00000137103.
GeneID	51754.
KEGG	hsa:51754.
UCSC	uc003zyk.3. human. uc003zym.3. human.
Organism-specific databases
CTD	51754.
GeneCards	GC09P035814.
HGNC	HGNC:21427. TMEM8B.
HPA	HPA052130.
neXtProt	NX_A6NDV4.
PharmGKB	PA165586305.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG77604.
OMA	TIAIRSH.
Gene expression databases
ArrayExpress	A6NDV4.
Bgee	A6NDV4.
CleanEx	HS_C9orf127.
Genevestigator	A6NDV4.
Family and domain databases
InterPro	IPR021910. DUF3522. IPR000742. EG-like_dom. IPR013032. EGF-like_CS. [Graphical view]
Pfam	PF12036. DUF3522. 1 hit. [Graphical view]
SMART	SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TMEM8B. human.
GenomeRNAi	51754.
NextBio	55850.

Entry information

Entry name

TMM8B_HUMAN

Accession

Primary (citable) accession number: A6NDV4
Secondary accession number(s): B3KQF3 , O75539, Q49AB1, Q4KMX5, Q5TCW5, Q9HBY2, Q9P0U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.