Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A6NDV4 (TMM8B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transmembrane protein 8B
Alternative name(s):
Nasopharyngeal carcinoma-associated gene 6 protein
Protein NAG-5
Protein NGX6
Gene names
Name:TMEM8B
Synonyms:C9orf127, NGX6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May function as a regulator of the EGFR pathway. Probable tumor suppressor which may function in cell growth, proliferation and adhesion. Ref.7 Ref.8 Ref.9 Ref.10

Subunit structure

Isoform 2 (via its cytoplasmic part) interacts with EZR. Ref.7 Ref.10

Subcellular location

Isoform 2: Cell membrane; Multi-pass membrane protein Probable. Cytoplasm Probable. Nucleus Probable. Mitochondrion. Endoplasmic reticulum. Note: Also detected in mitochondrion and endoplasmic reticulum. Ref.7 Ref.9

Induction

Isoform 2 is down-regulated in nasopharyngeal carcinoma cells. Ref.7

Post-translational modification

Isoform 2 is N-glycosylated. Ref.7

Sequence similarities

Belongs to the TMEM8 family.

Contains 1 EGF-like domain.

Sequence caution

The sequence AAC28644.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAF34820.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAF34820.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A6NDV4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A6NDV4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     274-337: FYHACDQPGI...GAMLLSMALQ → VCGGVCILSL...GHFCKTHWSK
     338-472: Missing.
Isoform 3 (identifier: A6NDV4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.
     82-93: GGVLSLELQLNA → MWRPHFHTCPPQ
     274-337: FYHACDQPGI...GAMLLSMALQ → VCGGVCILSL...GHFCKTHWSK
     338-472: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472Transmembrane protein 8B
PRO_0000333039

Regions

Topological domain1 – 233233Extracellular Potential
Transmembrane234 – 25421Helical; Potential
Topological domain255 – 2573Cytoplasmic Potential
Transmembrane258 – 27720Helical; Potential
Topological domain278 – 29215Extracellular Potential
Transmembrane293 – 31321Helical; Potential
Topological domain314 – 3152Cytoplasmic Potential
Transmembrane316 – 33621Helical; Potential
Topological domain337 – 3426Extracellular Potential
Transmembrane343 – 36321Helical; Potential
Topological domain364 – 37916Cytoplasmic Potential
Transmembrane380 – 40021Helical; Potential
Topological domain401 – 4055Extracellular Potential
Transmembrane406 – 42621Helical; Potential
Topological domain427 – 47246Cytoplasmic Potential
Domain182 – 22140EGF-like

Amino acid modifications

Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation1001N-linked (GlcNAc...) Potential
Disulfide bond186 ↔ 196 By similarity
Disulfide bond190 ↔ 209 By similarity
Disulfide bond211 ↔ 220 By similarity

Natural variations

Alternative sequence1 – 8181Missing in isoform 3.
VSP_033450
Alternative sequence82 – 9312GGVLS…LQLNA → MWRPHFHTCPPQ in isoform 3.
VSP_033451
Alternative sequence274 – 33764FYHAC…SMALQ → VCGGVCILSLGACAWWWVTV CISTTFSEGLGMSVPSLCLL QTETAVLPKLSCIDNGHFCK THWSK in isoform 2 and isoform 3.
VSP_033452
Alternative sequence338 – 472135Missing in isoform 2 and isoform 3.
VSP_033453

Experimental info

Sequence conflict1781R → W in AAH98296. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: 3E5EA4495CF54389

FASTA47251,941
        10         20         30         40         50         60 
MNMPQSLGNQ PLPPEPPSLG TPAEGPGTTS PPEHCWPVRP TLRNELDTFS VHFYIFFGPS 

        70         80         90        100        110        120 
VALPPERPAV FAMRLLPVLD SGGVLSLELQ LNASSVRQEN VTVFGCLTHE VPLSLGDAAV 

       130        140        150        160        170        180 
TCSKESLAGF LLSVSATTRV ARLRIPFPQT GTWFLALRSL CGVGPRFVRC RNATAEVRMR 

       190        200        210        220        230        240 
TFLSPCVDDC GPYGQCKLLR THNYLYAACE CKAGWRGWGC TDSADALTYG FQLLSTLLLC 

       250        260        270        280        290        300 
LSNLMFLPPV VLAIRSRYVL EAAVYTFTMF FSTFYHACDQ PGIVVFCIMD YDVLQFCDFL 

       310        320        330        340        350        360 
GSLMSVWVTV IAMARLQPVV KQVLYLLGAM LLSMALQLDR HGLWNLLGPS LFALGILATA 

       370        380        390        400        410        420 
WTVRSVRRRH CYPPTWRRWL FYLCPGSLIA GSAVLLYAFV ETRDNYFYIH SIWHMLIAGS 

       430        440        450        460        470 
VGFLLPPRAK TDHGVPSGAR ARGCGYQLCI NEQEELGLVG PGGATVSSIC AS 

« Hide

Isoform 2 [UniParc].

Checksum: BBB601E2F7ADAF3E
Show »

FASTA33836,844
Isoform 3 [UniParc].

Checksum: A482F1C8662472A8
Show »

FASTA25728,336

References

« Hide 'large scale' references
[1]"Proteomic detection of changes in protein synthesis induced by NGX6 transfected in human nasopharyngeal carcinoma cells."
Li J., Tan C., Xiang Q., Zhang X., Ma J., Wang J.-R., Yang J., Li W.-F., Shen S.-R., Liang S., Li G.-Y.
J. Protein Chem. 20:265-271(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]Yu W., Gibbs R.A.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Brain.
[7]"Role of a novel EGF-like domain-containing gene NGX6 in cell adhesion modulation in nasopharyngeal carcinoma cells."
Ma J., Zhou J., Fan S., Wang L.-L., Li X.-L., Yan Q., Zhou M., Liu H.-Y., Zhang Q., Zhou H., Gan K., Li Z., Peng C., Xiong W., Tan C., Shen S.-R., Yang J., Li J., Li G.-Y.
Carcinogenesis 26:281-291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH EZR, SUBCELLULAR LOCATION, GLYCOSYLATION, INDUCTION.
[8]"NGX6 gene inhibits cell proliferation and plays a negative role in EGFR pathway in nasopharyngeal carcinoma cells."
Wang L.-L., Ma J., Li J., Li X.-L., Zhang Q., Peng S.-P., Peng C., Zhou M., Xiong W., Yang J., Zhou J., Fan S., Tan C., Yan Q., Shen S.-R., Li G.-Y.
J. Cell. Biochem. 95:64-73(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"The role of NGX6 and its deletion mutants in the proliferation, adhesion and migration of nasopharyngeal carcinoma 5-8F cells."
Peng S.P., Li X.-L., Wang L., Ou-Yang J., Ma J., Wang L.-L., Liu H.-Y., Zhou M., Tang Y.-L., Li W.-S., Luo X.-M., Cao L., Tang K., Shen S.-R., Li G.-Y.
Oncology 71:273-281(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]"The expression of ezrin in NPC and its interaction with NGX6, a novel candidate suppressor."
Peng S.-P., Fan S., Li X.-L., Wang L., Liu H.-Y., Zhou M., Wang L.-L., Shen S.-R., Li G.-Y.
Cancer Sci. 98:341-349(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH EZR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF149297 mRNA. Translation: AAF34820.1. Sequence problems.
AF188239 mRNA. Translation: AAG29055.1.
AF070572 mRNA. Translation: AAC28644.1. Different initiation.
AK074844 mRNA. Translation: BAG52015.1.
AL133410 Genomic DNA. Translation: CAI10995.1.
AL133410 Genomic DNA. Translation: CAI10996.2.
CH471071 Genomic DNA. Translation: EAW58325.1.
BC041377 mRNA. No translation available.
BC098265 mRNA. Translation: AAH98265.1.
BC098296 mRNA. Translation: AAH98296.1.
BC099733 mRNA. Translation: AAH99733.1.
RefSeqNP_001036054.1. NM_001042589.2.
NP_001036055.1. NM_001042590.2.
NP_057530.2. NM_016446.3.
UniGeneHs.493808.

3D structure databases

ProteinModelPortalA6NDV4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119714. 1 interaction.
MINTMINT-2873602.

Proteomic databases

PaxDbA6NDV4.
PRIDEA6NDV4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000377988; ENSP00000367227; ENSG00000137103. [A6NDV4-1]
ENST00000377991; ENSP00000367230; ENSG00000137103. [A6NDV4-1]
ENST00000377996; ENSP00000367235; ENSG00000137103. [A6NDV4-2]
ENST00000439587; ENSP00000395810; ENSG00000137103. [A6NDV4-2]
GeneID51754.
KEGGhsa:51754.
UCSCuc003zyk.3. human. [A6NDV4-2]
uc003zym.4. human. [A6NDV4-1]

Organism-specific databases

CTD51754.
GeneCardsGC09P035814.
HGNCHGNC:21427. TMEM8B.
HPAHPA052130.
neXtProtNX_A6NDV4.
PharmGKBPA165586305.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG77604.
OMATIAIRSH.
OrthoDBEOG7FNC6S.
PhylomeDBA6NDV4.
TreeFamTF331003.

Gene expression databases

BgeeA6NDV4.
CleanExHS_C9orf127.
GenevestigatorA6NDV4.

Family and domain databases

InterProIPR021910. DUF3522.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
[Graphical view]
PfamPF12036. DUF3522. 1 hit.
[Graphical view]
SMARTSM00181. EGF. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTMEM8B. human.
GeneWikiC9orf127.
GenomeRNAi51754.
NextBio55850.
PROA6NDV4.

Entry information

Entry nameTMM8B_HUMAN
AccessionPrimary (citable) accession number: A6NDV4
Secondary accession number(s): B3KQF3 expand/collapse secondary AC list , O75539, Q49AB1, Q4KMX5, Q5TCW5, Q9HBY2, Q9P0U7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM