ID   ASPDH_HUMAN             Reviewed;         283 AA.
AC   A6ND91; Q6NZ37;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   27-MAR-2024, entry version 123.
DE   RecName: Full=Aspartate dehydrogenase domain-containing protein {ECO:0000305};
GN   Name=ASPDH {ECO:0000312|HGNC:HGNC:33856};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ARG-266.
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057824; DOI=10.1038/nature02399;
RA   Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA   Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA   Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA   Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA   Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA   Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA   Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA   Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA   Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA   McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA   Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA   Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA   She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA   Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA   Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA   Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA   Rubin E.M., Lucas S.M.;
RT   "The DNA sequence and biology of human chromosome 19.";
RL   Nature 428:529-535(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ARG-266.
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20 AND SER-168, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=A6ND91-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=A6ND91-2; Sequence=VSP_038742, VSP_038743;
CC   -!- SIMILARITY: Belongs to the L-aspartate dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; CH471135; EAW71880.1; -; Genomic_DNA.
DR   EMBL; AC008743; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC066359; AAH66359.1; -; mRNA.
DR   CCDS; CCDS33082.1; -. [A6ND91-2]
DR   CCDS; CCDS46153.1; -. [A6ND91-1]
DR   RefSeq; NP_001019827.2; NM_001024656.2. [A6ND91-2]
DR   RefSeq; NP_001108070.1; NM_001114598.1. [A6ND91-1]
DR   AlphaFoldDB; A6ND91; -.
DR   SMR; A6ND91; -.
DR   BioGRID; 299841; 16.
DR   IntAct; A6ND91; 3.
DR   MINT; A6ND91; -.
DR   STRING; 9606.ENSP00000373860; -.
DR   iPTMnet; A6ND91; -.
DR   PhosphoSitePlus; A6ND91; -.
DR   BioMuta; ASPDH; -.
DR   MassIVE; A6ND91; -.
DR   PaxDb; 9606-ENSP00000373860; -.
DR   PeptideAtlas; A6ND91; -.
DR   ProteomicsDB; 891; -. [A6ND91-1]
DR   ProteomicsDB; 892; -. [A6ND91-2]
DR   Antibodypedia; 46022; 82 antibodies from 12 providers.
DR   DNASU; 554235; -.
DR   Ensembl; ENST00000376916.7; ENSP00000366114.2; ENSG00000204653.10. [A6ND91-2]
DR   Ensembl; ENST00000389208.9; ENSP00000373860.3; ENSG00000204653.10. [A6ND91-1]
DR   GeneID; 554235; -.
DR   KEGG; hsa:554235; -.
DR   MANE-Select; ENST00000389208.9; ENSP00000373860.3; NM_001114598.2; NP_001108070.1.
DR   UCSC; uc002psr.4; human. [A6ND91-1]
DR   AGR; HGNC:33856; -.
DR   CTD; 554235; -.
DR   GeneCards; ASPDH; -.
DR   HGNC; HGNC:33856; ASPDH.
DR   HPA; ENSG00000204653; Tissue enhanced (brain, kidney, liver).
DR   neXtProt; NX_A6ND91; -.
DR   OpenTargets; ENSG00000204653; -.
DR   PharmGKB; PA164716234; -.
DR   VEuPathDB; HostDB:ENSG00000204653; -.
DR   eggNOG; ENOG502QVGC; Eukaryota.
DR   GeneTree; ENSGT00390000004452; -.
DR   HOGENOM; CLU_063528_0_0_1; -.
DR   InParanoid; A6ND91; -.
DR   OMA; HIVEVDV; -.
DR   OrthoDB; 2879851at2759; -.
DR   PhylomeDB; A6ND91; -.
DR   TreeFam; TF315092; -.
DR   PathwayCommons; A6ND91; -.
DR   SignaLink; A6ND91; -.
DR   BioGRID-ORCS; 554235; 19 hits in 1145 CRISPR screens.
DR   ChiTaRS; ASPDH; human.
DR   GenomeRNAi; 554235; -.
DR   Pharos; A6ND91; Tbio.
DR   PRO; PR:A6ND91; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; A6ND91; Protein.
DR   Bgee; ENSG00000204653; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 145 other cell types or tissues.
DR   ExpressionAtlas; A6ND91; baseline and differential.
DR   GO; GO:0033735; F:aspartate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0009435; P:NAD biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR005106; Asp/hSer_DH_NAD-bd.
DR   InterPro; IPR002811; Asp_DH.
DR   InterPro; IPR011182; L-Asp_DH.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR31873:SF6; ASPARTATE DEHYDROGENASE DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR31873; L-ASPARTATE DEHYDROGENASE-RELATED; 1.
DR   Pfam; PF01958; Asp_DH_C; 1.
DR   Pfam; PF03447; NAD_binding_3; 1.
DR   PIRSF; PIRSF005227; Asp_dh_NAD_syn; 1.
DR   SUPFAM; SSF55347; Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   Genevisible; A6ND91; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Phosphoprotein; Reference proteome.
FT   CHAIN           1..283
FT                   /note="Aspartate dehydrogenase domain-containing protein"
FT                   /id="PRO_0000300623"
FT   MOD_RES         20
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   MOD_RES         168
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   VAR_SEQ         2..65
FT                   /note="ADRGPWRVGVVGYGRLGQSLVSRLLAQGPELGLELVFVWNRDPGRMAGSVPP
FT                   SLQLQNLAALGE -> GDRVKGSKS (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_038742"
FT   VAR_SEQ         95..144
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_038743"
FT   VARIANT         266
FT                   /note="Q -> R (in dbSNP:rs12977172)"
FT                   /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.1"
FT                   /id="VAR_062676"
SQ   SEQUENCE   283 AA;  29946 MW;  FF4254EF3F14D840 CRC64;
     MADRGPWRVG VVGYGRLGQS LVSRLLAQGP ELGLELVFVW NRDPGRMAGS VPPSLQLQNL
     AALGERRPDL VVEVAHPKII HESGAQILRH ANLLVGSPSA LSDQTTERQL LEASQHWDHA
     VFVARGALWG AEDIRRLDAA GGLRSLRVTM ATHPDGFRLE GPLAAAHSPG PCTVLYEGPV
     RGLCPFAPRN SNTMAAAALA APSLGFDGVI GVLVADTSLT DMHVVDVELS GPRGPTGRSF
     AVHTRRENPA EPGAVTGSAT VTAFWQSLLA CCQLPSRPGI HLC
//