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A6ND01

- JUNO_HUMAN

UniProt

A6ND01 - JUNO_HUMAN

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Protein

Sperm-egg fusion protein Juno

Gene

FOLR4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for gamete recognition and fertilization. The IZUMO1:FOLR4/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate By similarity.By similarity

GO - Molecular functioni

  1. receptor activity Source: UniProtKB

GO - Biological processi

  1. cell adhesion Source: UniProtKB
  2. fusion of sperm to egg plasma membrane Source: UniProtKB
  3. single fertilization Source: UniProtKB
  4. sperm-egg recognition Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Fertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-egg fusion protein Juno
Alternative name(s):
Folate receptor 4
Folate receptor delta
Short name:
FR-delta
Gene namesi
Name:FOLR4
Synonyms:JUNO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:32565. FOLR4.

Subcellular locationi

Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity
Note: GPI-anchored at the oolemma.By similarity

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 228209Sperm-egg fusion protein JunoPRO_0000332987Add
BLAST
Propeptidei229 – 25022Sequence AnalysisPRO_0000429472Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 55By similarity
Disulfide bondi47 ↔ 95By similarity
Disulfide bondi56 ↔ 99By similarity
Glycosylationi73 – 731N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi79 ↔ 172By similarity
Disulfide bondi86 ↔ 143By similarity
Disulfide bondi132 ↔ 206By similarity
Disulfide bondi136 ↔ 186By similarity
Disulfide bondi149 ↔ 166By similarity
Lipidationi228 – 2281GPI-anchor amidated serineSequence Analysis

Post-translational modificationi

The protein is rapidly cleaved following fertilization, being only weakly detectable in zona-intact fertilized eggs at telophase II and undetectable at the pronuclear stage. Sheding is probably required to block to polyspermy and ensuring egg fusion with a single sperm By similarity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiA6ND01.
PRIDEiA6ND01.

Expressioni

Gene expression databases

BgeeiA6ND01.
GenevestigatoriA6ND01.

Interactioni

Subunit structurei

Interacts with IZUMO1; the interaction is direct.1 Publication

Protein-protein interaction databases

STRINGi9606.ENSP00000416935.

Structurei

3D structure databases

ProteinModelPortaliA6ND01.
SMRiA6ND01. Positions 18-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the folate receptor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG44097.
GeneTreeiENSGT00390000010470.
HOGENOMiHOG000006539.
HOVERGENiHBG039612.
InParanoidiA6ND01.
KOiK13649.
OMAiHYFPTPA.
OrthoDBiEOG7K6PW3.
PhylomeDBiA6ND01.
TreeFamiTF328532.

Family and domain databases

InterProiIPR004269. Folate_rcpt.
IPR018143. Folate_rcpt-like.
[Graphical view]
PANTHERiPTHR10517. PTHR10517. 1 hit.
PfamiPF03024. Folate_rec. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A6ND01-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MACWWPLLLE LWTVMPTWAG DELLNICMNA KHHKRVPSPE DKLYEECIPW
60 70 80 90 100
KDNACCTLTT SWEAHLDVSP LYNFSLFHCG LLMPGCRKHF IQAICFYECS
110 120 130 140 150
PNLGPWIQPV GSLGWEVAPS GQGERVVNVP LCQEDCEEWW EDCRMSYTCK
160 170 180 190 200
SNWRGGWDWS QGKNRCPKGA QCLPFSHYFP TPADLCEKTW SNSFKASPER
210 220 230 240 250
RNSGRCLQKW FEPAQGNPNV AVARLFASSA PSWELSYTIM VCSLFLPFLS

Note: No experimental confirmation available. Gene prediction based on EST data.

Length:250
Mass (Da):28,672
Last modified:January 11, 2011 - v3
Checksum:iCBF2FD967ABAF6E7
GO
Isoform 2 (identifier: A6ND01-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-116: Missing.

Note: No experimental confirmation available. Gene prediction based on similarity to orthologs.

Show »
Length:243
Mass (Da):27,943
Checksum:i814B8F7C842BAFBF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei110 – 1167Missing in isoform 2. CuratedVSP_040470

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP002784 Genomic DNA. No translation available.
RefSeqiNP_001186135.1. NM_001199206.1. [A6ND01-1]
UniGeneiHs.553758.

Genome annotation databases

EnsembliENST00000440961; ENSP00000416935; ENSG00000183560. [A6ND01-2]
GeneIDi390243.
KEGGihsa:390243.
UCSCiuc021qou.1. human. [A6ND01-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP002784 Genomic DNA. No translation available.
RefSeqi NP_001186135.1. NM_001199206.1. [A6ND01-1 ]
UniGenei Hs.553758.

3D structure databases

ProteinModelPortali A6ND01.
SMRi A6ND01. Positions 18-229.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000416935.

Proteomic databases

PaxDbi A6ND01.
PRIDEi A6ND01.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000440961 ; ENSP00000416935 ; ENSG00000183560 . [A6ND01-2 ]
GeneIDi 390243.
KEGGi hsa:390243.
UCSCi uc021qou.1. human. [A6ND01-1 ]

Organism-specific databases

CTDi 390243.
GeneCardsi GC11P094038.
HGNCi HGNC:32565. FOLR4.
MIMi 615737. gene.
neXtProti NX_A6ND01.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG44097.
GeneTreei ENSGT00390000010470.
HOGENOMi HOG000006539.
HOVERGENi HBG039612.
InParanoidi A6ND01.
KOi K13649.
OMAi HYFPTPA.
OrthoDBi EOG7K6PW3.
PhylomeDBi A6ND01.
TreeFami TF328532.

Miscellaneous databases

GenomeRNAii 390243.
NextBioi 103567.
PROi A6ND01.
SOURCEi Search...

Gene expression databases

Bgeei A6ND01.
Genevestigatori A6ND01.

Family and domain databases

InterProi IPR004269. Folate_rcpt.
IPR018143. Folate_rcpt-like.
[Graphical view ]
PANTHERi PTHR10517. PTHR10517. 1 hit.
Pfami PF03024. Folate_rec. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Juno is the egg Izumo receptor and is essential for mammalian fertilization."
    Bianchi E., Doe B., Goulding D., Wright G.J.
    Nature 508:483-487(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IZUMO1.

Entry informationi

Entry nameiJUNO_HUMAN
AccessioniPrimary (citable) accession number: A6ND01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 11, 2011
Last modified: October 29, 2014
This is version 55 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3