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A6ND01

- JUNO_HUMAN

UniProt

A6ND01 - JUNO_HUMAN

Protein

Sperm-egg fusion protein Juno

Gene

FOLR4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 3 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for gamete recognition and fertilization. The IZUMO1:FOLR4/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. receptor activity Source: UniProtKB

    GO - Biological processi

    1. cell adhesion Source: UniProtKB
    2. fusion of sperm to egg plasma membrane Source: UniProtKB
    3. single fertilization Source: UniProtKB
    4. sperm-egg recognition Source: UniProtKB

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Fertilization

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sperm-egg fusion protein Juno
    Alternative name(s):
    Folate receptor 4
    Folate receptor delta
    Short name:
    FR-delta
    Gene namesi
    Name:FOLR4
    Synonyms:JUNO
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:32565. FOLR4.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity
    Note: GPI-anchored at the oolemma.By similarity

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 228209Sperm-egg fusion protein JunoPRO_0000332987Add
    BLAST
    Propeptidei229 – 25022Sequence AnalysisPRO_0000429472Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi27 ↔ 55By similarity
    Disulfide bondi47 ↔ 95By similarity
    Disulfide bondi56 ↔ 99By similarity
    Glycosylationi73 – 731N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi79 ↔ 172By similarity
    Disulfide bondi86 ↔ 143By similarity
    Disulfide bondi132 ↔ 206By similarity
    Disulfide bondi136 ↔ 186By similarity
    Disulfide bondi149 ↔ 166By similarity
    Lipidationi228 – 2281GPI-anchor amidated serineSequence Analysis

    Post-translational modificationi

    The protein is rapidly cleaved following fertilization, being only weakly detectable in zona-intact fertilized eggs at telophase II and undetectable at the pronuclear stage. Sheding is probably required to block to polyspermy and ensuring egg fusion with a single sperm By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiA6ND01.
    PRIDEiA6ND01.

    Expressioni

    Gene expression databases

    BgeeiA6ND01.
    GenevestigatoriA6ND01.

    Interactioni

    Subunit structurei

    Interacts with IZUMO1; the interaction is direct.1 Publication

    Protein-protein interaction databases

    STRINGi9606.ENSP00000416935.

    Structurei

    3D structure databases

    ProteinModelPortaliA6ND01.
    SMRiA6ND01. Positions 18-229.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the folate receptor family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG44097.
    HOGENOMiHOG000006539.
    HOVERGENiHBG039612.
    InParanoidiA6ND01.
    KOiK13649.
    OMAiHYFPTPA.
    OrthoDBiEOG7K6PW3.
    PhylomeDBiA6ND01.
    TreeFamiTF328532.

    Family and domain databases

    InterProiIPR004269. Folate_rcpt.
    IPR018143. Folate_rcpt-like.
    [Graphical view]
    PANTHERiPTHR10517. PTHR10517. 1 hit.
    PfamiPF03024. Folate_rec. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A6ND01-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MACWWPLLLE LWTVMPTWAG DELLNICMNA KHHKRVPSPE DKLYEECIPW    50
    KDNACCTLTT SWEAHLDVSP LYNFSLFHCG LLMPGCRKHF IQAICFYECS 100
    PNLGPWIQPV GSLGWEVAPS GQGERVVNVP LCQEDCEEWW EDCRMSYTCK 150
    SNWRGGWDWS QGKNRCPKGA QCLPFSHYFP TPADLCEKTW SNSFKASPER 200
    RNSGRCLQKW FEPAQGNPNV AVARLFASSA PSWELSYTIM VCSLFLPFLS 250

    Note: No experimental confirmation available. Gene prediction based on EST data.

    Length:250
    Mass (Da):28,672
    Last modified:January 11, 2011 - v3
    Checksum:iCBF2FD967ABAF6E7
    GO
    Isoform 2 (identifier: A6ND01-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         110-116: Missing.

    Note: No experimental confirmation available. Gene prediction based on similarity to orthologs.

    Show »
    Length:243
    Mass (Da):27,943
    Checksum:i814B8F7C842BAFBF
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei110 – 1167Missing in isoform 2. CuratedVSP_040470

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP002784 Genomic DNA. No translation available.
    RefSeqiNP_001186135.1. NM_001199206.1. [A6ND01-1]
    UniGeneiHs.553758.

    Genome annotation databases

    EnsembliENST00000440961; ENSP00000416935; ENSG00000183560. [A6ND01-2]
    GeneIDi390243.
    KEGGihsa:390243.
    UCSCiuc021qou.1. human. [A6ND01-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP002784 Genomic DNA. No translation available.
    RefSeqi NP_001186135.1. NM_001199206.1. [A6ND01-1 ]
    UniGenei Hs.553758.

    3D structure databases

    ProteinModelPortali A6ND01.
    SMRi A6ND01. Positions 18-229.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000416935.

    Proteomic databases

    PaxDbi A6ND01.
    PRIDEi A6ND01.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000440961 ; ENSP00000416935 ; ENSG00000183560 . [A6ND01-2 ]
    GeneIDi 390243.
    KEGGi hsa:390243.
    UCSCi uc021qou.1. human. [A6ND01-1 ]

    Organism-specific databases

    CTDi 390243.
    GeneCardsi GC11P094038.
    HGNCi HGNC:32565. FOLR4.
    MIMi 615737. gene.
    neXtProti NX_A6ND01.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG44097.
    HOGENOMi HOG000006539.
    HOVERGENi HBG039612.
    InParanoidi A6ND01.
    KOi K13649.
    OMAi HYFPTPA.
    OrthoDBi EOG7K6PW3.
    PhylomeDBi A6ND01.
    TreeFami TF328532.

    Miscellaneous databases

    GenomeRNAii 390243.
    NextBioi 103567.
    PROi A6ND01.
    SOURCEi Search...

    Gene expression databases

    Bgeei A6ND01.
    Genevestigatori A6ND01.

    Family and domain databases

    InterProi IPR004269. Folate_rcpt.
    IPR018143. Folate_rcpt-like.
    [Graphical view ]
    PANTHERi PTHR10517. PTHR10517. 1 hit.
    Pfami PF03024. Folate_rec. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Juno is the egg Izumo receptor and is essential for mammalian fertilization."
      Bianchi E., Doe B., Goulding D., Wright G.J.
      Nature 508:483-487(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IZUMO1.

    Entry informationi

    Entry nameiJUNO_HUMAN
    AccessioniPrimary (citable) accession number: A6ND01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 54 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3