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Protein

Sperm-egg fusion protein Juno

Gene

IZUMO1R

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.By similarity

GO - Molecular functioni

  • receptor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Fertilization

Enzyme and pathway databases

BioCyciZFISH:G66-31023-MONOMER.

Protein family/group databases

TCDBi9.B.92.1.2. the folate receptor (fr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-egg fusion protein Juno
Alternative name(s):
Folate receptor 4
Folate receptor delta
Short name:
FR-delta
IZUMO1 receptor protein JUNO1 Publication
Gene namesi
Name:IZUMO1RImported
Synonyms:FOLR4, JUNO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:32565. IZUMO1R.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

  • Note: GPI-anchored at the oolemma.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45E → A: Nearly abolishes interaction with IZUMO1. 1 Publication1
Mutagenesisi45E → K: Abolishes interaction with IZUMO1. 1 Publication1
Mutagenesisi62W → A: Nearly abolishes interaction with IZUMO1. 2 Publications1
Mutagenesisi81L → A: Abolishes interaction with IZUMO1. 2 Publications1
Mutagenesisi163K → E: Mildly decreases interaction with IZUMO1. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000183560.

Polymorphism and mutation databases

BioMutaiFOLR4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000033298720 – 228Sperm-egg fusion protein JunoAdd BLAST209
PropeptideiPRO_0000429472229 – 250Sequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 55Combined sources2 Publications
Disulfide bondi47 ↔ 95Combined sources2 Publications
Disulfide bondi56 ↔ 99Combined sources2 Publications
Glycosylationi73N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi79 ↔ 172Combined sources2 Publications
Disulfide bondi86 ↔ 143Combined sources2 Publications
Disulfide bondi132 ↔ 206Combined sources2 Publications
Disulfide bondi136 ↔ 186Combined sources2 Publications
Disulfide bondi149 ↔ 166Combined sources2 Publications
Lipidationi228GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

The protein is rapidly cleaved following fertilization, being only weakly detectable in zona-intact fertilized eggs at telophase II and undetectable at the pronuclear stage. Sheding is probably required to block to polyspermy and ensuring egg fusion with a single sperm.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiA6ND01.
PRIDEiA6ND01.

Expressioni

Gene expression databases

ExpressionAtlasiA6ND01. baseline and differential.

Interactioni

Subunit structurei

Monomer (PubMed:27309808). Interacts with IZUMO1; the interaction is direct. IZUMO1 and IZUMO1R/JUNO form a complex with 1:1 stoichiometry (PubMed:24739963, PubMed:27309818, PubMed:27309808).3 Publications

Protein-protein interaction databases

DIPiDIP-62034N.
STRINGi9606.ENSP00000416935.

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 23Combined sources3
Beta strandi24 – 26Combined sources3
Beta strandi30 – 32Combined sources3
Helixi45 – 50Combined sources6
Beta strandi53 – 56Combined sources4
Helixi58 – 63Combined sources6
Beta strandi66 – 68Combined sources3
Turni70 – 72Combined sources3
Turni76 – 79Combined sources4
Helixi84 – 99Combined sources16
Helixi104 – 106Combined sources3
Beta strandi107 – 109Combined sources3
Beta strandi124 – 126Combined sources3
Beta strandi129 – 131Combined sources3
Helixi133 – 143Combined sources11
Beta strandi146 – 149Combined sources4
Beta strandi153 – 155Combined sources3
Helixi175 – 178Combined sources4
Helixi182 – 189Combined sources8
Turni190 – 192Combined sources3
Beta strandi193 – 196Combined sources4
Beta strandi203 – 207Combined sources5
Helixi213 – 215Combined sources3
Helixi220 – 227Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F4EX-ray2.40B20-228[»]
5F4QX-ray1.80A/B/C/D20-228[»]
5JKAX-ray2.00A/B20-228[»]
5JKBX-ray3.23A/B/C/D20-228[»]
5JKCX-ray2.90B20-228[»]
5JKDX-ray2.90B20-228[»]
5JKEX-ray2.86B/D20-228[»]
ProteinModelPortaliA6ND01.
SMRiA6ND01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni62 – 81Important for interaction with IZUMO12 PublicationsAdd BLAST20

Sequence similaritiesi

Belongs to the folate receptor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFFP. Eukaryota.
ENOG4111IU4. LUCA.
GeneTreeiENSGT00390000010470.
HOGENOMiHOG000006539.
HOVERGENiHBG039612.
InParanoidiA6ND01.
KOiK13649.
OMAiGRCLQKW.
OrthoDBiEOG091G0GIA.
PhylomeDBiA6ND01.
TreeFamiTF328532.

Family and domain databases

InterProiIPR004269. Folate_rcpt.
IPR018143. Folate_rcpt-like.
[Graphical view]
PANTHERiPTHR10517. PTHR10517. 1 hit.
PfamiPF03024. Folate_rec. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A6ND01-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MACWWPLLLE LWTVMPTWAG DELLNICMNA KHHKRVPSPE DKLYEECIPW
60 70 80 90 100
KDNACCTLTT SWEAHLDVSP LYNFSLFHCG LLMPGCRKHF IQAICFYECS
110 120 130 140 150
PNLGPWIQPV GSLGWEVAPS GQGERVVNVP LCQEDCEEWW EDCRMSYTCK
160 170 180 190 200
SNWRGGWDWS QGKNRCPKGA QCLPFSHYFP TPADLCEKTW SNSFKASPER
210 220 230 240 250
RNSGRCLQKW FEPAQGNPNV AVARLFASSA PSWELSYTIM VCSLFLPFLS
Note: No experimental confirmation available. Gene prediction based on EST data.
Length:250
Mass (Da):28,672
Last modified:January 11, 2011 - v3
Checksum:iCBF2FD967ABAF6E7
GO
Isoform 2 (identifier: A6ND01-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-116: Missing.

Note: No experimental confirmation available. Gene prediction based on similarity to orthologs.
Show »
Length:243
Mass (Da):27,943
Checksum:i814B8F7C842BAFBF
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040470110 – 116Missing in isoform 2. Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP002784 Genomic DNA. No translation available.
CCDSiCCDS81615.1. [A6ND01-1]
RefSeqiNP_001186135.1. NM_001199206.1. [A6ND01-1]
UniGeneiHs.553758.

Genome annotation databases

EnsembliENST00000328458; ENSP00000332963; ENSG00000183560. [A6ND01-1]
ENST00000440961; ENSP00000416935; ENSG00000183560. [A6ND01-2]
GeneIDi390243.
KEGGihsa:390243.
UCSCiuc058gou.1. human. [A6ND01-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP002784 Genomic DNA. No translation available.
CCDSiCCDS81615.1. [A6ND01-1]
RefSeqiNP_001186135.1. NM_001199206.1. [A6ND01-1]
UniGeneiHs.553758.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F4EX-ray2.40B20-228[»]
5F4QX-ray1.80A/B/C/D20-228[»]
5JKAX-ray2.00A/B20-228[»]
5JKBX-ray3.23A/B/C/D20-228[»]
5JKCX-ray2.90B20-228[»]
5JKDX-ray2.90B20-228[»]
5JKEX-ray2.86B/D20-228[»]
ProteinModelPortaliA6ND01.
SMRiA6ND01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-62034N.
STRINGi9606.ENSP00000416935.

Protein family/group databases

TCDBi9.B.92.1.2. the folate receptor (fr) family.

Polymorphism and mutation databases

BioMutaiFOLR4.

Proteomic databases

PaxDbiA6ND01.
PRIDEiA6ND01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328458; ENSP00000332963; ENSG00000183560. [A6ND01-1]
ENST00000440961; ENSP00000416935; ENSG00000183560. [A6ND01-2]
GeneIDi390243.
KEGGihsa:390243.
UCSCiuc058gou.1. human. [A6ND01-1]

Organism-specific databases

CTDi390243.
GeneCardsiIZUMO1R.
HGNCiHGNC:32565. IZUMO1R.
MIMi615737. gene.
neXtProtiNX_A6ND01.
OpenTargetsiENSG00000183560.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFFP. Eukaryota.
ENOG4111IU4. LUCA.
GeneTreeiENSGT00390000010470.
HOGENOMiHOG000006539.
HOVERGENiHBG039612.
InParanoidiA6ND01.
KOiK13649.
OMAiGRCLQKW.
OrthoDBiEOG091G0GIA.
PhylomeDBiA6ND01.
TreeFamiTF328532.

Enzyme and pathway databases

BioCyciZFISH:G66-31023-MONOMER.

Miscellaneous databases

GenomeRNAii390243.
PROiA6ND01.
SOURCEiSearch...

Gene expression databases

ExpressionAtlasiA6ND01. baseline and differential.

Family and domain databases

InterProiIPR004269. Folate_rcpt.
IPR018143. Folate_rcpt-like.
[Graphical view]
PANTHERiPTHR10517. PTHR10517. 1 hit.
PfamiPF03024. Folate_rec. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJUNO_HUMAN
AccessioniPrimary (citable) accession number: A6ND01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 11, 2011
Last modified: November 2, 2016
This is version 68 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.