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A6ND01 (JUNO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sperm-egg fusion protein Juno
Alternative name(s):
Folate receptor 4
Folate receptor delta
Short name=FR-delta
Gene names
Name:FOLR4
Synonyms:JUNO
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for gamete recognition and fertilization. The IZUMO1:FOLR4/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate By similarity.

Subunit structure

Interacts with IZUMO1; the interaction is direct. Ref.2

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor By similarity. Note: GPI-anchored at the oolemma By similarity.

Post-translational modification

The protein is rapidly cleaved following fertilization, being only weakly detectable in zona-intact fertilized eggs at telophase II and undetectable at the pronuclear stage. Sheding is probably required to block to polyspermy and ensuring egg fusion with a single sperm By similarity.

Sequence similarities

Belongs to the folate receptor family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A6ND01-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available. Gene prediction based on EST data.
Isoform 2 (identifier: A6ND01-2)

The sequence of this isoform differs from the canonical sequence as follows:
     110-116: Missing.
Note: No experimental confirmation available. Gene prediction based on similarity to orthologs.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 228209Sperm-egg fusion protein Juno
PRO_0000332987
Propeptide229 – 25022 Potential
PRO_0000429472

Amino acid modifications

Lipidation2281GPI-anchor amidated serine Potential
Glycosylation731N-linked (GlcNAc...) Potential
Disulfide bond27 ↔ 55 By similarity
Disulfide bond47 ↔ 95 By similarity
Disulfide bond56 ↔ 99 By similarity
Disulfide bond79 ↔ 172 By similarity
Disulfide bond86 ↔ 143 By similarity
Disulfide bond132 ↔ 206 By similarity
Disulfide bond136 ↔ 186 By similarity
Disulfide bond149 ↔ 166 By similarity

Natural variations

Alternative sequence110 – 1167Missing in isoform 2.
VSP_040470

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 11, 2011. Version 3.
Checksum: CBF2FD967ABAF6E7

FASTA25028,672
        10         20         30         40         50         60 
MACWWPLLLE LWTVMPTWAG DELLNICMNA KHHKRVPSPE DKLYEECIPW KDNACCTLTT 

        70         80         90        100        110        120 
SWEAHLDVSP LYNFSLFHCG LLMPGCRKHF IQAICFYECS PNLGPWIQPV GSLGWEVAPS 

       130        140        150        160        170        180 
GQGERVVNVP LCQEDCEEWW EDCRMSYTCK SNWRGGWDWS QGKNRCPKGA QCLPFSHYFP 

       190        200        210        220        230        240 
TPADLCEKTW SNSFKASPER RNSGRCLQKW FEPAQGNPNV AVARLFASSA PSWELSYTIM 

       250 
VCSLFLPFLS 

« Hide

Isoform 2 [UniParc].

Checksum: 814B8F7C842BAFBF
Show »

FASTA24327,943

References

« Hide 'large scale' references
[1]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Juno is the egg Izumo receptor and is essential for mammalian fertilization."
Bianchi E., Doe B., Goulding D., Wright G.J.
Nature 508:483-487(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IZUMO1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP002784 Genomic DNA. No translation available.
RefSeqNP_001186135.1. NM_001199206.1. [A6ND01-1]
UniGeneHs.553758.

3D structure databases

ProteinModelPortalA6ND01.
SMRA6ND01. Positions 18-229.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000416935.

Proteomic databases

PaxDbA6ND01.
PRIDEA6ND01.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000440961; ENSP00000416935; ENSG00000183560. [A6ND01-2]
GeneID390243.
KEGGhsa:390243.
UCSCuc021qou.1. human. [A6ND01-1]

Organism-specific databases

CTD390243.
GeneCardsGC11P094038.
HGNCHGNC:32565. FOLR4.
MIM615737. gene.
neXtProtNX_A6ND01.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG44097.
HOGENOMHOG000006539.
HOVERGENHBG039612.
InParanoidA6ND01.
KOK13649.
OMAHYFPTPA.
OrthoDBEOG7K6PW3.
PhylomeDBA6ND01.
TreeFamTF328532.

Gene expression databases

BgeeA6ND01.
GenevestigatorA6ND01.

Family and domain databases

InterProIPR004269. Folate_rcpt.
IPR018143. Folate_rcpt-like.
[Graphical view]
PANTHERPTHR10517. PTHR10517. 1 hit.
PfamPF03024. Folate_rec. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi390243.
NextBio103567.
PROA6ND01.
SOURCESearch...

Entry information

Entry nameJUNO_HUMAN
AccessionPrimary (citable) accession number: A6ND01
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 53 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM