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Protein

Piwi-like protein 1

Gene

PIWIL1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs (By similarity).By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei638By similarity1
Active sitei676By similarity1
Active sitei708By similarity1
Active sitei842By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Endonuclease, Hydrolase, Nuclease, RNA-binding
Biological processDifferentiation, Meiosis, RNA-mediated gene silencing, Translation regulation
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Piwi-like protein 1 (EC:3.1.26.-By similarity)
Gene namesi
Name:PIWIL1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003672861 – 867Piwi-like protein 1Add BLAST867

Post-translational modificationi

Methylated on arginine residues; required for the interaction with Tudor domain-containing protein and subsequent localization to the meiotic nuage, also named P granule.By similarity

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiA6N7Y9

Expressioni

Gene expression databases

BgeeiENSGALG00000002645

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000004162

Structurei

3D structure databases

SMRiA6N7Y9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini285 – 397PAZPROSITE-ProRule annotationAdd BLAST113
Domaini561 – 853PiwiPROSITE-ProRule annotationAdd BLAST293

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni324 – 326Required for binding 2'-O-methylated 3'-end of piRNAsBy similarity3
Regioni485 – 621MID regionBy similarityAdd BLAST137

Domaini

The PAZ domain specifically recognizes binds the 2'-O-methylated 3'-end of piRNAs. The MID region is required for recognition of uridine in the first position of piRNAs (g1U preference, also named 1U-bias).By similarity

Sequence similaritiesi

Belongs to the argonaute family. Piwi subfamily.Curated

Phylogenomic databases

eggNOGiKOG1042 Eukaryota
ENOG410XNRH LUCA
GeneTreeiENSGT00760000119148
HOGENOMiHOG000254789
HOVERGENiHBG049411
InParanoidiA6N7Y9
KOiK02156
OMAiPGYIQPR
OrthoDBiEOG091G020J
PhylomeDBiA6N7Y9

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR014811 ArgoL1
IPR031320 GAGE
IPR003100 PAZ_dom
IPR036085 PAZ_dom_sf
IPR003165 Piwi
IPR031326 PIWIL1
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR22891:SF46 PTHR22891:SF46, 1 hit
PfamiView protein in Pfam
PF08699 ArgoL1, 1 hit
PF05831 GAGE, 1 hit
PF02170 PAZ, 1 hit
PF02171 Piwi, 1 hit
SMARTiView protein in SMART
SM01379 GAGE, 1 hit
SM00949 PAZ, 1 hit
SM00950 Piwi, 1 hit
SUPFAMiSSF101690 SSF101690, 1 hit
SSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS50821 PAZ, 1 hit
PS50822 PIWI, 1 hit

Sequencei

Sequence statusi: Complete.

A6N7Y9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGRARARAR GRPPGQEAAI PPVGAASAQK TLPSHPSEQR QSLQPCHPPP
60 70 80 90 100
LTEEPGGRGR QRGPQDAPKT LGLQISAGFQ ELSLADRGGR RRDFHDLGVN
110 120 130 140 150
TRQAIEHVRE SKTGSSGAMI KLIANFFRLT SRPQWALYQY HVDYNPEMEA
160 170 180 190 200
RRLRSGLLFQ HEDLIGKTHA FDGSILFLPK RLPNKVTEVY SKTRNGEDVR
210 220 230 240 250
ITITLTNELP PTSPTCLQFY NIIFRRLLKM MNFQQIGRNY YNPKDPVSIP
260 270 280 290 300
NHRLMVWPGF TSSILQYEES IMLCADVSHK ILRSETVLDF MYSLYEQVEE
310 320 330 340 350
RRFRDACAKE LIGVIVLTKY NNRTYRVDDI DWDANPQCTF RRADGSEISY
360 370 380 390 400
IDYYKRQYNQ DISDLNQPVL ISQYRRKRGN VTVGPVVLIP ELCYLTGLTE
410 420 430 440 450
KMRNDFNMMK DLAVHTRLSP EQRQREIGKL VDCMKKDECV QKELRDWGLS
460 470 480 490 500
FDSSLLSFTG RVVQAEKILQ AGNVFDYNPQ FADWSRETRV APLIHAKPLD
510 520 530 540 550
NWLLIYTRRN YDAANMLLQN LFKVTPSMGI RMNKATMIEV DDRTEAYLRV
560 570 580 590 600
LQQSITPDTN IVVCILSSTR KDKYDAIKKY LCTDCPIPSQ CVVARTLSKP
610 620 630 640 650
QTALAIVTKI ALQMNCKMGG ELWSVEIPLK QLMIVGIDCY HDTLSGKQSI
660 670 680 690 700
AGFVASLNEK MTRWFSRCVV QSRGQEIVDG LKACLQTALR EWFKWNKYLP
710 720 730 740 750
SRIIVYRDGV GDGQLNTLVN YEVPQFLDCL KTVGKDYNPR LTVIVVKKRV
760 770 780 790 800
STRFFAQAGG GLKNPPPGTV VDIEVTRPEW YDFFIVSQAV RNGCVAPTHY
810 820 830 840 850
NVVYDTSKLK PDHVQRLTYK LCHMYYNWSG VIRVPAPCQY AHKLAFLVGQ
860
SIHREPNLLL SDRLYYL
Length:867
Mass (Da):99,286
Last modified:July 24, 2007 - v1
Checksum:iA8BAF115A1588475
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF619343 mRNA Translation: ABR09543.1
RefSeqiNP_001092322.1, NM_001098852.1
XP_015130744.1, XM_015275258.1
XP_015130745.1, XM_015275259.1
XP_015130746.1, XM_015275260.1
XP_015130747.1, XM_015275261.1
XP_015130748.1, XM_015275262.1
XP_015130749.1, XM_015275263.1
UniGeneiGga.16962

Genome annotation databases

EnsembliENSGALT00000004171; ENSGALP00000004162; ENSGALG00000002645
GeneIDi416804
KEGGigga:416804

Similar proteinsi

Entry informationi

Entry nameiPIWL1_CHICK
AccessioniPrimary (citable) accession number: A6N7Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 24, 2007
Last modified: October 25, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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