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A6MFL0

- OXLA_DEMVE

UniProt

A6MFL0 - OXLA_DEMVE

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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Demansia vestigiata (Lesser black whip snake) (Demansia atra)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, apoptosis of vascular endothelial cells or tumor cell lines, antibacterial and antiparasitic activities, as well as regulation of platelet aggregation. Its effect on platelets is controversial, since it either induces aggregation or inhibits agonist-induced aggregation. These different effects are probably due to different experimental conditions (By similarity).By similarity

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FAD.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901FADBy similarity
Binding sitei109 – 1091SubstrateBy similarity
Binding sitei280 – 2801FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei391 – 3911SubstrateBy similarity
Binding sitei476 – 4761FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 632FADBy similarity
Nucleotide bindingi82 – 832FADBy similarity
Nucleotide bindingi106 – 1094FADBy similarity
Nucleotide bindingi483 – 4886FADBy similarity
Nucleotide bindingi483 – 4842SubstrateBy similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB-KW
  3. hemolysis in other organism Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Toxin

Keywords - Biological processi

Apoptosis, Cytolysis, Hemolysis

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Short name:
LAAO
Short name:
LAO
OrganismiDemansia vestigiata (Lesser black whip snake) (Demansia atra)
Taxonomic identifieri412038 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeNotechinaeDemansia

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 517499L-amino-acid oxidasePRO_5000254114Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 ↔ 192By similarity
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi350 ↔ 431By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer; non-covalently linked.By similarity

Structurei

3D structure databases

ProteinModelPortaliA6MFL0.
SMRiA6MFL0. Positions 23-505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG005729.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00757. AMINEOXDASEF.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6MFL0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNVFFMFSLL FLAALESCAD DRRNPLGECF READYEEFVD IATNGLKQTS
60 70 80 90 100
NPKRVVVVGA GMAGLSAAYV LAGAGHNVML LEASERVGGR VNTYRNEQEG
110 120 130 140 150
WYVNLGPMRL PERHRIVREY IKKFGLQLNQ FFQEDEDAWY FIKNIRKKVR
160 170 180 190 200
EVKENPSIFP YPVKPSEKGK SAPQLYRDSL QKIIEEYGRS NCSYILNKYD
210 220 230 240 250
TYSTKDYLIK EGNLSPGAVD MIGDLLNEGS SYYLSFIESL KSDDIFSYEN
260 270 280 290 300
RFDEIVGGFD LLPKAMYKAI EEKVHLNARV IQIQQNAEGV RVTYQTPAKN
310 320 330 340 350
LSYVTADYVI VCSTSRAARR IYFEPPLPPE KAHALQSIHY RSATKIFLTC
360 370 380 390 400
TKKFWEDDGI HGGKSITDRP SRLIHYPNHN FPNGIGVLVI YTIADDADFF
410 420 430 440 450
LALDNKTIAD IVIHDLSLIH QLPKEKIRDL CYVSMIKKWS LDKYSMGSIT
460 470 480 490 500
TFAPYQFQEY FETVAAPVGR IYFAGEYTAR AHGWIDSTIK SGLKAARDVN
510
RASQKPSRIQ LSNDNEL
Length:517
Mass (Da):58,920
Last modified:July 24, 2007 - v1
Checksum:i474BB53222E72C22
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ917521 mRNA. Translation: ABK63550.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ917521 mRNA. Translation: ABK63550.1 .

3D structure databases

ProteinModelPortali A6MFL0.
SMRi A6MFL0. Positions 23-505.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG005729.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
PRINTSi PR00757. AMINEOXDASEF.
ProtoNeti Search...

Publicationsi

  1. "Diversity of toxic components from the venom of the evolutionarily distinct black whip snake, Demansia vestigiata."
    St Pierre L., Birrell G.W., Earl S.T.H., Wallis T.P., Gorman J.J., de Jersey J., Masci P.P., Lavin M.F.
    J. Proteome Res. 6:3093-3107(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Venom gland.

Entry informationi

Entry nameiOXLA_DEMVE
AccessioniPrimary (citable) accession number: A6MFL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 24, 2007
Last modified: October 29, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3