Reviewed, UniProtKB/Swiss-Prot A6MFL0 (OXLA_DEMVE)

Names and origin

Protein names	Recommended name:     L-amino-acid oxidase       Short name=LAAO       Short name=LAO     EC=1.4.3.2
Organism	Demansia vestigiata (Lesser black whip snake) (Demansia atra)
Taxonomic identifier	412038 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Notechinae › Demansia

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids. Inhibits platelet aggregation. Has an ability to induce apoptosis and hemorrhage. Has an antibacterial activity By similarity.
Catalytic activity	An L-amino acid + H(2)O + O(2) = a 2-oxo acid + NH(3) + H(2)O(2).
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	SecretedBy similarity.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Glycosylated By similarity.
Sequence similarities	Belongs to the flavin monoamine oxidase family. FIG1 subfamily.

Ontologies

Keywords
Biological process	Apoptosis Blood coagulation
Cellular component	Secreted
Domain	Signal
Ligand	FAD Flavoprotein
Molecular function	Antibiotic Antimicrobial Oxidoreductase Toxin
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-amino-acid oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 517	499	L-amino-acid oxidase		PRO_5000254114
Regions
	Nucleotide binding	108 – 109	2	FAD By similarity
	Nucleotide binding	485 – 488	4	FAD By similarity
Sites
	Binding site	82	1	FAD By similarity
	Binding site	90	1	FAD By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	280	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	391	1	Substrate By similarity
	Binding site	476	1	FAD By similarity
Amino acid modifications
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Disulfide bond	29 ↔ 192		By similarity
	Disulfide bond	350 ↔ 431		By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6MFL0-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 474BB53222E72C22	FASTA	517	58,920
	10 20 30 40 50 60 MNVFFMFSLL FLAALESCAD DRRNPLGECF READYEEFVD IATNGLKQTS NPKRVVVVGA 70 80 90 100 110 120 GMAGLSAAYV LAGAGHNVML LEASERVGGR VNTYRNEQEG WYVNLGPMRL PERHRIVREY 130 140 150 160 170 180 IKKFGLQLNQ FFQEDEDAWY FIKNIRKKVR EVKENPSIFP YPVKPSEKGK SAPQLYRDSL 190 200 210 220 230 240 QKIIEEYGRS NCSYILNKYD TYSTKDYLIK EGNLSPGAVD MIGDLLNEGS SYYLSFIESL 250 260 270 280 290 300 KSDDIFSYEN RFDEIVGGFD LLPKAMYKAI EEKVHLNARV IQIQQNAEGV RVTYQTPAKN 310 320 330 340 350 360 LSYVTADYVI VCSTSRAARR IYFEPPLPPE KAHALQSIHY RSATKIFLTC TKKFWEDDGI 370 380 390 400 410 420 HGGKSITDRP SRLIHYPNHN FPNGIGVLVI YTIADDADFF LALDNKTIAD IVIHDLSLIH 430 440 450 460 470 480 QLPKEKIRDL CYVSMIKKWS LDKYSMGSIT TFAPYQFQEY FETVAAPVGR IYFAGEYTAR 490 500 510 AHGWIDSTIK SGLKAARDVN RASQKPSRIQ LSNDNEL

References

[1]

"Diversity of toxic components from the venom of the evolutionarily distinct black whip snake, Demansia vestigiata." St Pierre L., Birrell G.W., Earl S.T.H., Wallis T.P., Gorman J.J., de Jersey J., Masci P.P., Lavin M.F. J. Proteome Res. 6:3093-3107(2007) [PubMed: 17608513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Venom gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ917521 mRNA. Translation: ABK63550.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...

Entry information

Entry name

OXLA_DEMVE

Accession

Primary (citable) accession number: A6MFL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	November 25, 2008
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)