Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A6MFL0

- OXLA_DEMVE

UniProt

A6MFL0 - OXLA_DEMVE

Protein

L-amino-acid oxidase

Gene
N/A
Organism
Demansia vestigiata (Lesser black whip snake) (Demansia atra)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 31 (01 Oct 2014)
      Sequence version 1 (24 Jul 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, apoptosis of vascular endothelial cells or tumor cell lines, antibacterial and antiparasitic activities, as well as regulation of platelet aggregation. Its effect on platelets is controversial, since it either induces aggregation or inhibits agonist-induced aggregation. These different effects are probably due to different experimental conditions By similarity.By similarity

    Catalytic activityi

    An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

    Cofactori

    FAD.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei90 – 901FADBy similarity
    Binding sitei109 – 1091SubstrateBy similarity
    Binding sitei280 – 2801FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei391 – 3911SubstrateBy similarity
    Binding sitei476 – 4761FADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi62 – 632FADBy similarity
    Nucleotide bindingi82 – 832FADBy similarity
    Nucleotide bindingi106 – 1094FADBy similarity
    Nucleotide bindingi483 – 4886FADBy similarity
    Nucleotide bindingi483 – 4842SubstrateBy similarity

    GO - Molecular functioni

    1. L-amino-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. defense response to bacterium Source: UniProtKB-KW
    3. hemolysis in other organism Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Toxin

    Keywords - Biological processi

    Apoptosis, Cytolysis, Hemolysis

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-amino-acid oxidase (EC:1.4.3.2)
    Short name:
    LAAO
    Short name:
    LAO
    OrganismiDemansia vestigiata (Lesser black whip snake) (Demansia atra)
    Taxonomic identifieri412038 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeNotechinaeDemansia

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 517499L-amino-acid oxidasePRO_5000254114Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi29 ↔ 192By similarity
    Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi350 ↔ 431By similarity

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Interactioni

    Subunit structurei

    Homodimer; non-covalently linked.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA6MFL0.
    SMRiA6MFL0. Positions 23-505.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG005729.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00757. AMINEOXDASEF.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A6MFL0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNVFFMFSLL FLAALESCAD DRRNPLGECF READYEEFVD IATNGLKQTS    50
    NPKRVVVVGA GMAGLSAAYV LAGAGHNVML LEASERVGGR VNTYRNEQEG 100
    WYVNLGPMRL PERHRIVREY IKKFGLQLNQ FFQEDEDAWY FIKNIRKKVR 150
    EVKENPSIFP YPVKPSEKGK SAPQLYRDSL QKIIEEYGRS NCSYILNKYD 200
    TYSTKDYLIK EGNLSPGAVD MIGDLLNEGS SYYLSFIESL KSDDIFSYEN 250
    RFDEIVGGFD LLPKAMYKAI EEKVHLNARV IQIQQNAEGV RVTYQTPAKN 300
    LSYVTADYVI VCSTSRAARR IYFEPPLPPE KAHALQSIHY RSATKIFLTC 350
    TKKFWEDDGI HGGKSITDRP SRLIHYPNHN FPNGIGVLVI YTIADDADFF 400
    LALDNKTIAD IVIHDLSLIH QLPKEKIRDL CYVSMIKKWS LDKYSMGSIT 450
    TFAPYQFQEY FETVAAPVGR IYFAGEYTAR AHGWIDSTIK SGLKAARDVN 500
    RASQKPSRIQ LSNDNEL 517
    Length:517
    Mass (Da):58,920
    Last modified:July 24, 2007 - v1
    Checksum:i474BB53222E72C22
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ917521 mRNA. Translation: ABK63550.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ917521 mRNA. Translation: ABK63550.1 .

    3D structure databases

    ProteinModelPortali A6MFL0.
    SMRi A6MFL0. Positions 23-505.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG005729.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00757. AMINEOXDASEF.
    ProtoNeti Search...

    Publicationsi

    1. "Diversity of toxic components from the venom of the evolutionarily distinct black whip snake, Demansia vestigiata."
      St Pierre L., Birrell G.W., Earl S.T.H., Wallis T.P., Gorman J.J., de Jersey J., Masci P.P., Lavin M.F.
      J. Proteome Res. 6:3093-3107(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Venom gland.

    Entry informationi

    Entry nameiOXLA_DEMVE
    AccessioniPrimary (citable) accession number: A6MFL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 31 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3