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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBEI290402:GHL5-4692-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Cbei_4590
OrganismiClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
Taxonomic identifieri290402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000565 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 816816Leucine--tRNA ligasePRO_1000074828Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290402.Cbei_4590.

Structurei

3D structure databases

ProteinModelPortaliA6M268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A6M268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANYGTKIDE KWQKFWDENE VYKFNPENSG KKLYTLEMFS YPSGAQLHAG
60 70 80 90 100
HWFNYGPTDS WARLKRMQGY NVFQPMGFDA FGLPAENYAI KTGIHPQDST
110 120 130 140 150
LKNIETMEKQ LKSMGAMFNW ENEVVTCLPD YYKWTQWLFL KLYEKGLAYR
160 170 180 190 200
KKAPVNWCPS CNTVLANEQV VDGVCERCST EVTKKDLTQW FFKITEYGDE
210 220 230 240 250
LLDKLDGLDW PEKTKSMQKH WIGRSYGAEV TFKVKDSDLK FDVFTTRVDT
260 270 280 290 300
LNGVTYVVLA PENKLVDELT IPEYKAAVEE YKEAAAKQSE IERQSVSKEK
310 320 330 340 350
TGVFTGSYAI NPINGKVVPI WISDYVLATY GTGCVMAVPA HDERDFAFAT
360 370 380 390 400
KFNLPIERVI TDKENTNPDL PYCEYGVLVN SGKFDGLTTD EAKKKIVEEL
410 420 430 440 450
EKDELGAMKK NFRLRDWLVS RQRYWGAPIP VIYCDDCGIV PVPEKDLPVK
460 470 480 490 500
LPYNVEFTPD GKSPLGKCED FVNTTCPHCG KPAKREADTL DTFVCSSFYY
510 520 530 540 550
LRYVDNKNDD APFDSEKVNK MLPVDKYVGG PEHACMHLLY ARFITKALRD
560 570 580 590 600
MGYLNFDEPF LSLTHQGLIL GPDGLKMSKS KGNTISPDDY IKEYGADVFR
610 620 630 640 650
MYLMFGFGYT EGGAWSDDGI KSVGKFVDRI ERILENCRNI INSNESTKDS
660 670 680 690 700
IDSAEKELNF WKHNTIKGVT EDGDKMQFNT AIARLMELTN ALNKYTQENI
710 720 730 740 750
KNANFLKETI VDFIKLLAPF APHFAEEQWS LLGNNSTIFN EKWPEFNPAA
760 770 780 790 800
LVKDEVEIAI QINGKIKAKI MVASNLDEEG IKAASLENET IKENTEGKTI
810
VKVIVIKGRL VNIVVK
Length:816
Mass (Da):92,942
Last modified:July 24, 2007 - v1
Checksum:iF6D655DD20488AA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000721 Genomic DNA. Translation: ABR36698.1.
RefSeqiWP_012060745.1. NC_009617.1.
YP_001311654.1. NC_009617.1.

Genome annotation databases

EnsemblBacteriaiABR36698; ABR36698; Cbei_4590.
KEGGicbe:Cbei_4590.
PATRICi19353506. VBICloBei69853_4768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000721 Genomic DNA. Translation: ABR36698.1.
RefSeqiWP_012060745.1. NC_009617.1.
YP_001311654.1. NC_009617.1.

3D structure databases

ProteinModelPortaliA6M268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290402.Cbei_4590.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR36698; ABR36698; Cbei_4590.
KEGGicbe:Cbei_4590.
PATRICi19353506. VBICloBei69853_4768.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCBEI290402:GHL5-4692-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51743 / NCIMB 8052.

Entry informationi

Entry nameiSYL_CLOB8
AccessioniPrimary (citable) accession number: A6M268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 24, 2007
Last modified: April 29, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.