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A6M1Z4 (ASSY_CLOB8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:Cbei_4515
OrganismClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) [Complete proteome] [HAMAP]
Taxonomic identifier290402 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000329467

Regions

Nucleotide binding10 – 189ATP By similarity

Sites

Binding site881Citrulline By similarity
Binding site931Citrulline By similarity
Binding site1181ATP; via amide nitrogen By similarity
Binding site1201Aspartate By similarity
Binding site1241Aspartate By similarity
Binding site1241Citrulline By similarity
Binding site1251Aspartate By similarity
Binding site1281Citrulline By similarity
Binding site1771Citrulline By similarity
Binding site1861Citrulline By similarity
Binding site2631Citrulline By similarity
Binding site2751Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
A6M1Z4 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 929F290FE923B91B

FASTA40745,628
        10         20         30         40         50         60 
MIKYNKVVLA YSGGLDTSII IPWLKENYGC EVIAVCGNVG QKDELDGLEE KAIKTGASKL 

        70         80         90        100        110        120 
YIEDLTQEFV DDYIFPTIQA GAIYEGKYLL GTSFARPIIG KRLVEIAKAE GADAICHGCT 

       130        140        150        160        170        180 
GKGNDQVRFE MAVKAFAPEM PIIAPWRIWD IKSREDEIDY AEAKNVPLKI NRETNYSKDK 

       190        200        210        220        230        240 
NIWHLSHEGL DLEFPENEPQ YDKILELCKT LEAAPNEATY ITLSFENGIA IALNGKKMNS 

       250        260        270        280        290        300 
VELLDELNKI GGENAIGITD MVENRLVGMK SRGVYETPGG TILYKAHKDL EELCLDKETA 

       310        320        330        340        350        360 
HYKEQIALKF ADLVYNGQWF TPLREALTEF VNKTQETVTG EIKLKLYKGN IVNAGMTSPY 

       370        380        390        400 
SLYSEEYATF GEDGVYDQKD SAGFINLYGL PTVVKAKMQE KIKKEEM 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000721 Genomic DNA. Translation: ABR36624.1.
RefSeqYP_001311580.1. NC_009617.1.

3D structure databases

ProteinModelPortalA6M1Z4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290402.Cbei_4515.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR36624; ABR36624; Cbei_4515.
GeneID5295670.
KEGGcbe:Cbei_4515.
PATRIC19353352. VBICloBei69853_4691.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAIYNGYWW.
OrthoDBEOG6K9QCV.
ProtClustDBPRK00509.

Enzyme and pathway databases

BioCycCBEI290402:GHL5-4617-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_CLOB8
AccessionPrimary (citable) accession number: A6M1Z4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 24, 2007
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways