Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isocitrate dehydrogenase [NADP]

Gene

Cbei_4199

Organism
Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771SubstrateUniRule annotation
Binding sitei82 – 821NADPUniRule annotation
Binding sitei109 – 1091SubstrateUniRule annotation
Binding sitei132 – 1321SubstrateUniRule annotation
Sitei139 – 1391Critical for catalysisUniRule annotation
Sitei210 – 2101Critical for catalysisUniRule annotation
Metal bindingi250 – 2501Magnesium or manganeseUniRule annotation
Binding sitei258 – 2581NADPUniRule annotation
Metal bindingi273 – 2731Magnesium or manganeseUniRule annotation
Binding sitei325 – 3251NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 773NADPUniRule annotation
Nucleotide bindingi307 – 3126NADPUniRule annotation

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. isocitrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

BioCyciCBEI290402:GHL5-4301-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Ordered Locus Names:Cbei_4199Imported
OrganismiClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)Imported
Taxonomic identifieri290402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000565: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi290402.Cbei_4199.

Structurei

3D structure databases

ProteinModelPortaliA6M129.
SMRiA6M129. Positions 3-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 1007Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0538.
HOGENOMiHOG000019858.
KOiK00031.
OMAiHNFESCG.
OrthoDBiEOG628F3X.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6M129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKIKMSTPL VEMDGDEMTR IIWGAIKEEL LNPFIELNTE YYDLGLEYRN
60 70 80 90 100
ETDDQVTVDA AEAIKKYKVG VKCATITPNA ARVKEYNLKE MWKSPNGTIR
110 120 130 140 150
AILDGTVFRA PIIVEPVKPY VRTWKKPITI ARHAYGDVYR DVEMKIEGKG
160 170 180 190 200
KCELVFTSET GEEKRELVHN FAGDGVVLGM HNLNKSIESF ARACFNFALD
210 220 230 240 250
TKQDLWFATK DTISKKYDHT FKDIFQDIFD DEYKIKFDEA GIEYFYTLID
260 270 280 290 300
DVVARVVKSE GGIIWACKNY DGDVMSDMVA TAFGSLAMMT SVLVSPEGNY
310 320 330 340 350
EYEAAHGTVQ RHYYKHLQGE ETSTNSIATI FAWSGALKKR GELDNNADLV
360 370 380 390 400
NFADKLEKAS IKTIEDGIMT KDLSLLAEHD NIQTLNTFDF IKAIRKTLEE

IL
Length:402
Mass (Da):45,692
Last modified:July 24, 2007 - v1
Checksum:i047EC017DE1C8D6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000721 Genomic DNA. Translation: ABR36309.1.
RefSeqiWP_012060356.1. NC_009617.1.
YP_001311265.1. NC_009617.1.

Genome annotation databases

EnsemblBacteriaiABR36309; ABR36309; Cbei_4199.
GeneIDi5295355.
KEGGicbe:Cbei_4199.
PATRICi19352690. VBICloBei69853_4360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000721 Genomic DNA. Translation: ABR36309.1.
RefSeqiWP_012060356.1. NC_009617.1.
YP_001311265.1. NC_009617.1.

3D structure databases

ProteinModelPortaliA6M129.
SMRiA6M129. Positions 3-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290402.Cbei_4199.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR36309; ABR36309; Cbei_4199.
GeneIDi5295355.
KEGGicbe:Cbei_4199.
PATRICi19352690. VBICloBei69853_4360.

Phylogenomic databases

eggNOGiCOG0538.
HOGENOMiHOG000019858.
KOiK00031.
OMAiHNFESCG.
OrthoDBiEOG628F3X.

Enzyme and pathway databases

BioCyciCBEI290402:GHL5-4301-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51743 / NCIMB 8052Imported.

Entry informationi

Entry nameiA6M129_CLOB8
AccessioniPrimary (citable) accession number: A6M129
Entry historyi
Integrated into UniProtKB/TrEMBL: July 24, 2007
Last sequence update: July 24, 2007
Last modified: February 4, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.