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Protein

Biotin synthase

Gene

bioB

Organism
Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi64Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi67Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi104Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi136Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi196Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi266Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBEI290402:G1G93-2006-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Cbei_1914
OrganismiClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
Taxonomic identifieri290402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000565 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003813101 – 316Biotin synthaseAdd BLAST316

Proteomic databases

PRIDEiA6LUQ4

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi290402.Cbei_1914

Structurei

3D structure databases

ProteinModelPortaliA6LUQ4
SMRiA6LUQ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

A6LUQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKLAEEIIN GRRLTRSDDL EFLLTCDLKK ICDGANKIRK TLCGESVDLC
60 70 80 90 100
TIINGRSGKC SENCKFCAQS NHHKTEIKKY DFLDPNVILK DCKKNEANGV
110 120 130 140 150
HRYSIVTAGR ALVGSDFDKA VQAYKMMNKE CSINLCASHG FLSEEQFVLL
160 170 180 190 200
KEAGVTMYHA NIETSKRNFH NICTTHSYDD KIKEIKLAQN AGLKVCSGGI
210 220 230 240 250
IGMGETWYDR IDMAISLSEL GVISIPINVL MPIKGTPLEN LKRISNYDIL
260 270 280 290 300
RTIAIFRYIN PTAYIRMAAG RTYFEDGGIE IFQSGSNATI TGDMLTTVGN
310
NTCQDKIMLQ TLGFSI
Length:316
Mass (Da):35,213
Last modified:July 24, 2007 - v1
Checksum:iC2AAA9111ECEDC20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000721 Genomic DNA Translation: ABR34084.1
RefSeqiWP_012058145.1, NC_009617.1

Genome annotation databases

EnsemblBacteriaiABR34084; ABR34084; Cbei_1914
KEGGicbe:Cbei_1914

Similar proteinsi

Entry informationi

Entry nameiBIOB_CLOB8
AccessioniPrimary (citable) accession number: A6LUQ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 24, 2007
Last modified: May 23, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health