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A6LRN7 (TRMB_CLOB8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Cbei_0833
OrganismClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) [Complete proteome] [HAMAP]
Taxonomic identifier290402 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000084438

Sites

Binding site431S-adenosyl-L-methionine By similarity
Binding site681S-adenosyl-L-methionine By similarity
Binding site1011S-adenosyl-L-methionine By similarity
Binding site1241S-adenosyl-L-methionine By similarity
Binding site1281Substrate By similarity
Binding site1601Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A6LRN7 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 95982451BB683741

FASTA21925,972
        10         20         30         40         50         60 
MRMRKKPWAR PELEGCDFFV INPKEYKGKW KEFFGNDKPI YLELGCGKGT FMAVHASENP 

        70         80         90        100        110        120 
DINYIAIDIK DEVLGLAKRN IEKAYEEKNR KTDNVKLMAQ EIGLISEILS EEDVVSRIYI 

       130        140        150        160        170        180 
NFCNPWPKEK HKKRRLTHMR QLEQYKTFLK SEGEIYFKTD DDELFEESLE YFNEAGFRIK 

       190        200        210 
YITYDLHNSD VEGNVQTEHE KMFSEQGIKI KFLIAMKDN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000721 Genomic DNA. Translation: ABR33017.1.
RefSeqYP_001307973.1. NC_009617.1.

3D structure databases

ProteinModelPortalA6LRN7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290402.Cbei_0833.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR33017; ABR33017; Cbei_0833.
GeneID5292061.
KEGGcbe:Cbei_0833.
PATRIC19345620. VBICloBei69853_0856.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAKTDDDEL.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycCBEI290402:GHL5-904-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CLOB8
AccessionPrimary (citable) accession number: A6LRN7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 24, 2007
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways