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A6LQY8 (DDL_CLOB8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Cbei_0581
OrganismClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) [Complete proteome] [HAMAP]
Taxonomic identifier290402 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074769

Regions

Domain99 – 294196ATP-grasp
Nucleotide binding126 – 18156ATP By similarity

Sites

Metal binding2481Magnesium or manganese 1 By similarity
Metal binding2611Magnesium or manganese 1 By similarity
Metal binding2611Magnesium or manganese 2 By similarity
Metal binding2631Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A6LQY8 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 3349E9CF27A2EA17

FASTA30133,290
        10         20         30         40         50         60 
MKIGVIMGGI SSEREISLQS GKSIIENINK EKYEVLPIVI DKKEDIINKS KGIDFALLAL 

        70         80         90        100        110        120 
HGQFGEDGTV QSVLQTLGIP YSGCGPLSSA MCMDKDVSKS VLEANGIRTA PWINLRKGDN 

       130        140        150        160        170        180 
IDFNKINELG YPVVVKPTHG GSSVATFIVK EEKEIENCVS EAFKWDSEVM IEKFIKGDEI 

       190        200        210        220        230        240 
TCPVYNNKML PVIAIKPKAE FFDFTSKYQD GGAEEIVVEL EAELHKEVEK MALETYKALK 

       250        260        270        280        290        300 
CEVYSRVDMI VTEEGVPYIL EINTLPGMTK NSLIPKSAAA LNIGFSELID MIIQDSLKIS 


R 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000721 Genomic DNA. Translation: ABR32768.1.
RefSeqYP_001307724.1. NC_009617.1.

3D structure databases

ProteinModelPortalA6LQY8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290402.Cbei_0581.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR32768; ABR32768; Cbei_0581.
GeneID5291812.
KEGGcbe:Cbei_0581.
PATRIC19345092. VBICloBei69853_0597.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAKYTEGFA.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycCBEI290402:GHL5-646-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CLOB8
AccessionPrimary (citable) accession number: A6LQY8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 24, 2007
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways