A6LQF2 (ISPE_CLOB8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Cbei_0394

Organism

Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) [Complete proteome] [HAMAP]

Taxonomic identifier

290402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000075043
Regions
	Nucleotide binding	91 – 101	11	ATP Potential
Sites
	Active site	8	1	By similarity
	Active site	133	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6LQF2 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 65730CBEC35FDAAC	FASTA	280	31,414
	10 20 30 40 50 60 MKIKAYAKIN IALDIVGKRE DGYHILKMIM QTIDLYDIIE IEKTESEIRL NCNKHYVPTD 70 80 90 100 110 120 ERNLAYKAAK IFKETYSISQ GVDIKLTKNI PVSAGLAGGS TDAAGVLKLM NKMFNVNASD 130 140 150 160 170 180 EELKSIGLRL GADVPYCIKG GTALCEGIGE KITQLKSFKD KIIVLVKPPF GVSTKEVYKC 190 200 210 220 230 240 FDLSKVVFHP KIDSLIENMN NDDVYFVANN MKNLLENVTL RKHRVITNIK EEMKSIGSIG 250 260 270 280 TMMSGSGPTV FALFDDMLKA QLCYDEMKKK YKDVFITRTI

References

[1]

"Complete sequence of Clostridium beijerinckii NCIMB 8052." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Tapia R., Brainard J., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Bennet G., Cann I., Chen J.-S., Contreras A.L., Jones D., Kashket E., Mitchell W., Stoddard S., Schwarz W., Qureshi N., Young M., Shi Z., Ezeji T., White B., Blaschek H., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51743 / NCIMB 8052.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000721 Genomic DNA. Translation: ABR32582.1.
RefSeq	YP_001307538.1. NC_009617.1.
3D structure databases
ProteinModelPortal	A6LQF2.
ModBase	Search...
Protein-protein interaction databases
STRING	A6LQF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5291626.
GenomeReviews	Gene locus Cbei_0394 in contig CP000721_GR.
KEGG	cbe:Cbei_0394.
PATRIC	19344694. VBICloBei69853_0407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	VSTAEVY.
ProtClustDB	PRK00128.
Enzyme and pathway databases
BioCyc	CBEI290402:CBEI_0394-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CLOB8

Accession

Primary (citable) accession number: A6LQF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 24, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program