Reviewed, UniProtKB/Swiss-Prot A6LPL1 (FTHS_CLOB8)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase
      Short name=FHS
      Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Cbei_0101

Organism

Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) [Complete proteome] [HAMAP]

Taxonomic identifier

290402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_1000087650
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6LPL1-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: D32E9899A16C3302	FASTA	556	60,270
	10 20 30 40 50 60 MKSDIQIAQE AKMEPIKNVA EKLGLCEDDI EYYGKYKCKI SLDVYDKVKN NNNGKLVLVT 70 80 90 100 110 120 AINPTPAGEG KSTVTVGLGQ ALNRIGKKAV IALREPSLGP VFGIKGGAAG GGYAQVVPME 130 140 150 160 170 180 DINLHFTGDM HAITSANNLL SAAIDNHIHQ GNVLRIDSRR IVFKRVMDMN DRALRHIIVG 190 200 210 220 230 240 MGGKVNGFVR EDGFNITVAS EIMAILCLAS DLEDLKERMG NIVIAYNLDG NPVYAKELEI 250 260 270 280 290 300 QGAMALLMKD AIKPNLVQTL ENTPALIHGG PFANIAHGCN SIMATKLALK LGDVVITEAG 310 320 330 340 350 360 FGADLGAEKF FDIKCRYGNL EPECVVVVAT IRALKHHGGV AKTELNIPNV EALKDGIANL 370 380 390 400 410 420 EKQIENIKKF KITPVVAINK FVTDSSEEVE FIKDFCDRIG VKVALCDVWA KGGEGGIDLA 430 440 450 460 470 480 NIVLDALENS ESNFEPIYDK EKSIREKIFT IASEIYGADK VNYTPAAKKQ IDELEKFKLD 490 500 510 520 530 540 KLPICMAKTQ YSLSDNPSLL ARPTGFDITV KEVRVSNGAG FIVVQTGDIM TMPGLPKVPA 550 ANKMDVLKSG EIIGLF

References

[1]

"Complete sequence of Clostridium beijerinckii NCIMB 8052." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Tapia R., Brainard J., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Bennet G., Cann I., Chen J.-S., Contreras A.L., Jones D., Kashket E., Mitchell W., Stoddard S., Schwarz W., Qureshi N., Young M., Shi Z., Ezeji T., White B., Blaschek H., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000721 Genomic DNA. Translation: ABR32291.1.
RefSeq	YP_001307247.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6LPL1.
Genome annotation databases
GeneID	5291335.
GenomeReviews	Gene locus Cbei_0101 in contig CP000721_GR.
KEGG	cbe:Cbei_0101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GMKGGAT.
Family and domain databases
HAMAP	MF_01543. [Tree]
InterPro	IPR000559. For_THF_ligase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_CLOB8

Accession

Primary (citable) accession number: A6LPL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)