Reviewed, UniProtKB/Swiss-Prot A6LPI6 (SYN_CLOB8)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	Cbei_0076

Organism

Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) [Complete proteome] [HAMAP]

Taxonomic identifier

290402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm By similarity HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000128203

Sequences

	Sequence		Length	Mass (Da)
	A6LPI6-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 5D548FBF8C943D7A	FASTA	464	53,311
	10 20 30 40 50 60 MAKSILIRSL YRNTDDFLSK EITISGWIRT LRSSNAFGFI EVNDGSFFKN IQVVFDDKLN 70 80 90 100 110 120 NFKEISKLPI SSSISVIGIL VATPEAKQPF EIQAKNIIIE GMSNSDYPLQ KKRHTFEYLR 130 140 150 160 170 180 TIAHLRPRSN AFAATFRVRS VAAYAIHKFF QDQNFVYTHT PIITGSDCEG AGEMFRVTTL 190 200 210 220 230 240 DPKTPELSKD GSVDFSKDFF GKETNLTVSG QLNAECFALA FRNIYTFGPT FRAENSNTTR 250 260 270 280 290 300 HAAEFWMIEP EIAFADLQDD MELAENMLKY VIKYVMDECP EELEFFNQFV DKGLLERLNH 310 320 330 340 350 360 VVSSDFAKVT YTEAVEILQK CGKKFDYDVS WGIDLQTEHE RYLTEEHFKK PLFVTDYPKD 370 380 390 400 410 420 IKAFYMRLND DGKTVAATDL LVPGIGEIIG GSQREERLDV LKDRMTELNL SEDDYWWYLE 430 440 450 460 LRKYGETKHA GFGLGFERLI MYITGMTNIR DVVPFPRTPG TSEF

References

[1]

"Complete sequence of Clostridium beijerinckii NCIMB 8052." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Tapia R., Brainard J., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Bennet G., Cann I., Chen J.-S., Contreras A.L., Jones D., Kashket E., Mitchell W., Stoddard S., Schwarz W., Qureshi N., Young M., Shi Z., Ezeji T., White B., Blaschek H., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000721 Genomic DNA. Translation: ABR32266.1.
RefSeq	YP_001307222.1.
3D structure databases
SMR	A6LPI6. Positions 14-462.
ModBase	Search...
Protein-protein interaction databases
STRING	A6LPI6.
Genome annotation databases
GeneID	5291310.
GenomeReviews	Gene locus Cbei_0076 in contig CP000721_GR.
KEGG	cbe:Cbei_0076.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0017.
HOGENOM	HBG745843.
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR016027. NA-bd_OB-fold-like. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_CLOB8

Accession

Primary (citable) accession number: A6LPI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 24, 2007
Last modified:	February 9, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)