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A6LPF6 (A6LPF6_CLOB8) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS HAMAP MF_02003
Ordered Locus Names:Cbei_0046
OrganismClostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) [Complete proteome] [HAMAP] EMBL ABR32236.1
Taxonomic identifier290402 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length1037 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
A6LPF6 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: EF1C3DC76AB000C1

FASTA1,037119,117
        10         20         30         40         50         60 
MYNKVDSSKG TVKIEKDIAK LWTEKDIIKK SFDSNSDGEY FTFYDGPPTA NGKPHVGHIL 

        70         80         90        100        110        120 
TRVMKDIIPR YKVMKGYKVL RKAGWDTHGL PVELEIEKKL GISGKEQIED FGVEKFVHEC 

       130        140        150        160        170        180 
KDSVFSYVSL WKEMSEQIGF WVDMDDPYVT YHDNYIESEW WALKQMWNKG LLYKGHKVMP 

       190        200        210        220        230        240 
YCPRCGTALS SHEVAQGYKD VKDLTATVKF KVKGEENKYI LAWTTTPWTL PSNLALCINK 

       250        260        270        280        290        300 
SYTYAEVKVG EEIYILAKDL VGKVLGDTEY EIVKEFKGEE LLGTEYEQLL PFAKVEGKAF 

       310        320        330        340        350        360 
VVIHGDYVTL SDGTGIVHIA PAYGEDDSLV SKKNGIAFIN LVDTNGNFVE EVTPWAGKFV 

       370        380        390        400        410        420 
KKCDESIVKY LEERNQLFTA QKHTHSYPHC WRCDTPLLYY PKDSWFVAMT KMRDKLLENN 

       430        440        450        460        470        480 
NKINWYPDNT RTGRMGKFLE NVIDWGISRD RYWGTPLPIW QCECDHQECI GSREELEEKA 

       490        500        510        520        530        540 
TTDCKGIELH KPYVDGIKLK CPHCGKEMKR THEVIDCWFD SGSMPFAQWH YPFENKETFE 

       550        560        570        580        590        600 
KNFPAQFISE AVDQTRGWFY TLLAISTCLF DTNSFENCIV LGHVLDKKGL KMSKHKGNVV 

       610        620        630        640        650        660 
DPFEVLNSQG ADATRWHFYT ASAPWLPTRF SIDDVAEAQR KFLGTLWNVY SFYVLYAEID 

       670        680        690        700        710        720 
KFDSTKYSDF VSDNIMDKWI ISKLNTLIET VDDGLNTYKI TQAALAIEDF TDELSNWYVR 

       730        740        750        760        770        780 
RNRSRYWSQE LTNDKIGAYV TLYKVLTNLV KVASPFVPFM TEEIYQNLVV NLDKNAPESI 

       790        800        810        820        830        840 
HLCTWPEVDK SAINKELEKE MDLAYTLVKL GRSARNAANV KNRQPLSELL ISTKSLPDYY 

       850        860        870        880        890        900 
GDIVKEELNI KKVDLGADLS KYVNFEIKPN LPVIGKLYGK LIPQIRKAIS EKNQMELAQK 

       910        920        930        940        950        960 
IQNGGSETII VNDTEIVLTN ENLLVTMQGL EGYAFAGEGE LGVVLDTTVT PELQEEGHVR 

       970        980        990       1000       1010       1020 
EVISKIQNMR KDKGFEVADR INLYVSNNDM LIDVIKKFEQ TIKKETLTCE VLYNQESNYS 

      1030 
ETVINSETLN MNVEVVR

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References

[1]"Complete sequence of Clostridium beijerinckii NCIMB 8052."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Tapia R., Brainard J., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Mikhailova N., Bennet G., Cann I., Chen J.-S., Contreras A.L., Jones D., Kashket E., Mitchell W., Stoddard S., Schwarz W., Qureshi N., Young M., Shi Z., Ezeji T., White B., Blaschek H., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51743 / NCIMB 8052.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000721 Genomic DNA. Translation: ABR32236.1.
RefSeqYP_001307192.1. NC_009617.1.

3D structure databases

ProteinModelPortalA6LPF6.
ModBaseSearch...

Protein-protein interaction databases

STRINGA6LPF6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5291280.
GenomeReviewsGene locus Cbei_0046 in contig CP000721_GR.
KEGGcbe:Cbei_0046.
PATRIC19343880. VBICloBei69853_0046.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHBG577712.
OMAPYITYDN.
ProtClustDBPRK06039.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 2 hits.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA6LPF6_CLOB8
AccessionPrimary (citable) accession number: A6LPF6
Entry history
Integrated into UniProtKB/TrEMBL: July 24, 2007
Last sequence update: July 24, 2007
Last modified: January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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