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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Thermosipho melanesiensis (strain BI429 / DSM 12029)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi493 – 4931MagnesiumUniRule annotation
Metal bindingi499 – 4991MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciTMEL391009:GHM1-1674-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Tmel_1612
OrganismiThermosipho melanesiensis (strain BI429 / DSM 12029)
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho
ProteomesiUP000001110 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 699699Polyribonucleotide nucleotidyltransferasePRO_0000329911Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391009.Tmel_1612.

Structurei

3D structure databases

ProteinModelPortaliA6LNF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini560 – 62061KHUniRule annotationAdd
BLAST
Domaini630 – 69768S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6LNF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFREWSKE FFGRKLTIQY GKVAKQSSGA AWVQFGDSVV LATANISDRV
60 70 80 90 100
IEGVDFVPLT VEYQEKFYAA GKIPGGFIKR EGKPTESAIL SARLIDRPIR
110 120 130 140 150
PLFPKYLRND VQLIVTVLSV DGDTPPDVTG IFAASLALNF SKIPFQGIVA
160 170 180 190 200
GVRIGYVDGE FVIFPTEEQL KNSKLDIVVA GSKDAITMVE GEAKEVTEEE
210 220 230 240 250
MVQALMVAHE AIKKLIEFEE EILREFNVEK MEIEEPKPKD ELIGRFEELL
260 270 280 290 300
VENELRKRLL IKEKLERSLK LKEYKEELIS KIFEEFEIDD EEKLTQEMLL
310 320 330 340 350
KELFDEKAKK LMRKIIINEG IRADGRTPEE IRPITCEVGV LPRTHGSALF
360 370 380 390 400
TRGETQSLGI VTLGAPMEEQ IVDTIMEEGT KRFILHYNFP PFSVGEVKPL
410 420 430 440 450
RGPGRREIGH GHLAERALKA VAPDEEDFPY VVRVVSEILE SNGSSSMATV
460 470 480 490 500
CSGSLALMDA GVPIKTHVAG VAMGLIVDEE NEVVLTDIQG LEDHWGDMDF
510 520 530 540 550
KVAGTRNGIT AFQMDCKIAG VGEELLKKAL KQARVARMRI LDIMFETIKE
560 570 580 590 600
PRKSLSPYAP LIANIEIDPM KVGELIGPGG KVIKSIVKEF DVEISIDDVT
610 620 630 640 650
GKVSVYGKDQ NKVNQAIEYI KTLTREIEVG DMFEGKITRI EPYGLFVELM
660 670 680 690
PGKIGLAHSS KLGNDSKEFR KKYKVGDVIK VVVVNIDDSG RIQLGKAEE
Length:699
Mass (Da):78,031
Last modified:July 24, 2007 - v1
Checksum:i32BA1193D8A26D06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA. Translation: ABR31456.1.
RefSeqiYP_001306841.1. NC_009616.1.

Genome annotation databases

EnsemblBacteriaiABR31456; ABR31456; Tmel_1612.
KEGGitme:Tmel_1612.
PATRICi23924515. VBITheMel19269_1671.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA. Translation: ABR31456.1.
RefSeqiYP_001306841.1. NC_009616.1.

3D structure databases

ProteinModelPortaliA6LNF5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391009.Tmel_1612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR31456; ABR31456; Tmel_1612.
KEGGitme:Tmel_1612.
PATRICi23924515. VBITheMel19269_1671.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciTMEL391009:GHM1-1674-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BI429 / DSM 12029.

Entry informationi

Entry nameiPNP_THEM4
AccessioniPrimary (citable) accession number: A6LNF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 24, 2007
Last modified: April 1, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.