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Protein

NAD kinase

Gene

nadK

Organism
Thermosipho melanesiensis (strain BI429 / DSM 12029)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei51 – 511Proton acceptorUniRule annotation
Binding sitei56 – 561NADUniRule annotation
Binding sitei124 – 1241NADUniRule annotation
Binding sitei140 – 1401NADUniRule annotation
Binding sitei142 – 1421NADUniRule annotation
Binding sitei177 – 1771NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi51 – 522NADUniRule annotation
Nucleotide bindingi113 – 1142NADUniRule annotation
Nucleotide bindingi153 – 1586NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciTMEL391009:GHM1-1602-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Tmel_1541
OrganismiThermosipho melanesiensis (strain BI429 / DSM 12029)
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho
ProteomesiUP000001110: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251NAD kinasePRO_1000005452Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391009.Tmel_1541.

Structurei

3D structure databases

ProteinModelPortaliA6LN85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiERTDILG.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

A6LN85-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLGIFHKP SLKSVAEKFS EILFDENFHV EYVGSEIPSI EVDLTLVLGG
60 70 80 90 100
DGTFLKAAHK VRNPLVGFKG GRLGFLSSYT LGDFDKFLED LKNENFERDI
110 120 130 140 150
RYFLKAGDFY TLNEVLLIRD PVQKMVDIQI FFQDGDFYFH ADGLIISTPT
160 170 180 190 200
GSTGYSLSLG GPIMLPNVNS FVITPVAPQF LASRSIIVPD DEEIIVRIDQ
210 220 230 240 250
EINLILDGMD FGKVREVNLK KSRRRIVILR PKDYNFSKSI KEKLGYGKRF

L
Length:251
Mass (Da):28,453
Last modified:July 24, 2007 - v1
Checksum:i913D3822AAA77512
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA. Translation: ABR31386.1.
RefSeqiYP_001306771.1. NC_009616.1.

Genome annotation databases

EnsemblBacteriaiABR31386; ABR31386; Tmel_1541.
GeneIDi5297193.
KEGGitme:Tmel_1541.
PATRICi23924371. VBITheMel19269_1600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA. Translation: ABR31386.1.
RefSeqiYP_001306771.1. NC_009616.1.

3D structure databases

ProteinModelPortaliA6LN85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391009.Tmel_1541.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR31386; ABR31386; Tmel_1541.
GeneIDi5297193.
KEGGitme:Tmel_1541.
PATRICi23924371. VBITheMel19269_1600.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiERTDILG.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciTMEL391009:GHM1-1602-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BI429 / DSM 12029.

Entry informationi

Entry nameiNADK_THEM4
AccessioniPrimary (citable) accession number: A6LN85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 24, 2007
Last modified: January 7, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.