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Protein

8-amino-7-oxononanoate synthase

Gene

Tmel_1346

Organism
Thermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.By similarity

Catalytic activityi

Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein].

Cofactori

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei133SubstrateBy similarity1
Binding sitei164SubstrateBy similarity1
Binding sitei180Pyridoxal phosphateBy similarity1
Binding sitei211Pyridoxal phosphateBy similarity1
Binding sitei353SubstrateBy similarity1
Binding sitei384SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciTMEL391009:GHM1-1370-MONOMER.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
8-amino-7-oxononanoate synthase (EC:2.3.1.47)
Short name:
AONS
Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name:
7-KAP synthase
Short name:
KAPA synthase
8-amino-7-ketopelargonate synthase
Alpha-oxoamine synthase
Gene namesi
Ordered Locus Names:Tmel_1346
OrganismiThermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeThermosipho
Proteomesi
  • UP000001110 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003811221 – 3918-amino-7-oxononanoate synthaseAdd BLAST391

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei239N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi391009.Tmel_1346.

Structurei

3D structure databases

ProteinModelPortaliA6LMP4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 109Pyridoxal phosphate bindingBy similarity2
Regioni139 – 140Pyridoxal phosphate bindingBy similarity2
Regioni205 – 208Pyridoxal phosphate bindingBy similarity4
Regioni236 – 239Pyridoxal phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.
HOGENOMiHOG000221022.
KOiK00639.
OMAiEGSRFKL.
OrthoDBiPOG091H024U.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR010962. AONS_Archaea/Firmicutes.
IPR004723. AONS_Archaea/Proteobacteria.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00858. bioF. 1 hit.
TIGR01825. gly_Cac_T_rel. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6LMP4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDYSIFAKE IDELKNQGLY TYIRTLESPQ GAWLVIDGKK VLNLCSNNYL
60 70 80 90 100
GFANEERLKN AAKQAVEKWG VGPGAVRTIA GTFSLHNELE ETLAKFKKVE
110 120 130 140 150
ATIFLQSGFV ANQAVIPAIT NEEDAILSDE LNHASIIDGV RLSKAKRFVW
160 170 180 190 200
KHRDIKDLEE KLKEAKDARR KLIITDGVFS MDGDLAPLPE IVELAEKYNA
210 220 230 240 250
MVMVDDAHGE GVLGSHGRGI VDHFGLHGRV DIEIGTLSKA FGVLGGYIAG
260 270 280 290 300
KKELIDYLKQ KARPFLFSSP LSPADTAAAL EATKILQESD ERVKRLWDNA
310 320 330 340 350
KYFKEEMKKL GFDTGESETP ITPVMLYDAK LSTQFSKELF EEGIFAQSIG
360 370 380 390
YPTVPKGKAR IRVMISAVHT KEDLDFALEK FEKVGKKLGV I
Length:391
Mass (Da):43,502
Last modified:July 24, 2007 - v1
Checksum:i9125A9B0EF2ADB2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA. Translation: ABR31195.1.
RefSeqiWP_012057554.1. NC_009616.1.

Genome annotation databases

EnsemblBacteriaiABR31195; ABR31195; Tmel_1346.
KEGGitme:Tmel_1346.
PATRICi23923957. VBITheMel19269_1398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA. Translation: ABR31195.1.
RefSeqiWP_012057554.1. NC_009616.1.

3D structure databases

ProteinModelPortaliA6LMP4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391009.Tmel_1346.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR31195; ABR31195; Tmel_1346.
KEGGitme:Tmel_1346.
PATRICi23923957. VBITheMel19269_1398.

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.
HOGENOMiHOG000221022.
KOiK00639.
OMAiEGSRFKL.
OrthoDBiPOG091H024U.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciTMEL391009:GHM1-1370-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR010962. AONS_Archaea/Firmicutes.
IPR004723. AONS_Archaea/Proteobacteria.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00858. bioF. 1 hit.
TIGR01825. gly_Cac_T_rel. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIOF_THEM4
AccessioniPrimary (citable) accession number: A6LMP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 24, 2007
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.