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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Thermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei230ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTMEL391009:G1G91-1224-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:Tmel_1176
OrganismiThermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeThermosipho
Proteomesi
  • UP000001110 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003677871 – 462Glutamate--tRNA ligase 2Add BLAST462

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391009.Tmel_1176

Structurei

3D structure databases

ProteinModelPortaliA6LM79
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi8 – 18"HIGH" regionUniRule annotationAdd BLAST11
Motifi227 – 231"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
1.10.1160.10, 1 hit
1.10.8.70, 1 hit
3.40.50.620, 2 hits
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020752 aa-tRNA-synth_I_codon-bd_sub1
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020061 Glu_tRNA_lig_a-bdl
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 2 hits
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A6LM79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRLRFAPSP TGLLHVGGAR TALFNWLYAK KHNGKFIIRI EDTDTERSTK
60 70 80 90 100
EYETKILSAL EWLGLNWDEG PDIGGGVGPY RQSERLHIYQ DIAQKLINEK
110 120 130 140 150
LAYYAVYDGE NEIHRSFEYP KKFKDKSIVV KFKVVKEDKT NFHDLLKGEM
160 170 180 190 200
SFENKFFNDF IIIKSNGFPT YNFAVVVDDH FMKISHVFRG EDHLSNTPKQ
210 220 230 240 250
IMIYNALGWK NPTFMHIPLI LGNDKTPLSK RHGGTSVDFF KESGILNNAL
260 270 280 290 300
LNYLAILGWH VDEEIFNVKK KIHTFDYRNI SNKSVVFDYK KLEWLNGQHM
310 320 330 340 350
RNLDIEELII KFKEFLKLKN YNLNIDETLE YTKDVIIICR EKVNTLSQLF
360 370 380 390 400
EISFSFFTEE YNYEENYIKK FLKKKETGDI LRKAIESFEK LENYEISSIE
410 420 430 440 450
NTLRKIVEDM NLATKKVFQT IRGALLGKLV TPGLFESIEV LGKEKTLKRL
460
KKTLDIWEKY EV
Length:462
Mass (Da):54,217
Last modified:July 24, 2007 - v1
Checksum:iC62AD07A9128A06A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA Translation: ABR31030.1
RefSeqiWP_012057389.1, NC_009616.1

Genome annotation databases

EnsemblBacteriaiABR31030; ABR31030; Tmel_1176
KEGGitme:Tmel_1176

Similar proteinsi

Entry informationi

Entry nameiSYE2_THEM4
AccessioniPrimary (citable) accession number: A6LM79
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 24, 2007
Last modified: March 28, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health