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A6LKA2 (DDL_THEM4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Tmel_0486
OrganismThermosipho melanesiensis (strain BI429 / DSM 12029) [Complete proteome] [HAMAP]
Taxonomic identifier391009 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030516

Regions

Domain139 – 336198ATP-grasp
Nucleotide binding167 – 22256ATP By similarity

Sites

Metal binding2961Magnesium or manganese 1 By similarity
Metal binding3071Magnesium or manganese 1 By similarity
Metal binding3071Magnesium or manganese 2 By similarity
Metal binding3091Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A6LKA2 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: AA24E4FCADFC1E01

FASTA36141,621
        10         20         30         40         50         60 
MLNIGIFFGS KSVEHEISII TAQQVLSSID RKKYNVIPIY ISKSGEWFTG KVLEDLETFK 

        70         80         90        100        110        120 
DFDKLEKKAK KIDSFSVKNN KLLLKYGLKK QTIDFCFLVF HGTNGEDGTF QGMCEIFGIP 

       130        140        150        160        170        180 
YSGCNHFSSA FTMDKVVTKL LLKEKEISVV DFEYTTEINE EFFKRCEKNL GYPMIVKPAR 

       190        200        210        220        230        240 
LGSSIGVSKV VDRKNFEEAV KNVLLFDNKV LVEKWINARE INCAVMGYKN IFVSELEEIN 

       250        260        270        280        290        300 
KKNDFFNFEE KYFKKGKKFS NHIIPARIDE NLKNKIKEIA KDTFKILECS GNVRIDFLIA 

       310        320        330        340        350        360 
DKIYVNEINT IPGALSFYIW QKSGFTFSQI IDNMINMGLE RFKDKKIVSI DTNILKIKVG 


K 

« Hide

References

[1]"Complete sequence of Thermosipho melanesiensis BI429."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BI429 / DSM 12029.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000716 Genomic DNA. Translation: ABR30353.1.
RefSeqYP_001305738.1. NC_009616.1.

3D structure databases

ProteinModelPortalA6LKA2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391009.Tmel_0486.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR30353; ABR30353; Tmel_0486.
GeneID5298236.
KEGGtme:Tmel_0486.
PATRIC23922131. VBITheMel19269_0511.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAIECGVLG.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycTMEL391009:GHM1-510-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_THEM4
AccessionPrimary (citable) accession number: A6LKA2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 24, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways