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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei547Aminoacyl-adenylateUniRule annotation1
Binding sitei591ATPUniRule annotation1
Metal bindingi876ZincUniRule annotation1
Metal bindingi879ZincUniRule annotation1
Metal bindingi896ZincUniRule annotation1
Metal bindingi899ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTMEL391009:G1G91-487-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Tmel_0461
OrganismiThermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeThermosipho
Proteomesi
  • UP000001110 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221371 – 908Isoleucine--tRNA ligaseAdd BLAST908

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391009.Tmel_0461

Structurei

3D structure databases

ProteinModelPortaliA6LK77
SMRiA6LK77
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi588 – 592"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A6LK77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLNLP STEFSMRANL VKKEPEMIKK WHEMDDYNLI LKSREGKPKF
60 70 80 90 100
VLHDGPPYAN GNIHIGTATN KILKDIVIRY KTMRGYYAPY VPGWDTHGLP
110 120 130 140 150
IEHRVSVEMG EKIKGMSPVE IRQKCKEFAL HFVDVQREQF KRLGVRGDWD
160 170 180 190 200
NPYLTLDPKY EKHILNIFKT LVKNGNVYRG NKPVYWCPTC KTALAEAEVE
210 220 230 240 250
YHDHSSPSIY VKFELEKNTY IVIWTTTPWT LPANVAIAVH PEYDYVKIKV
260 270 280 290 300
GNEYWIVAEG LLNKFAAETE IEYSVVEKFK GKDLEHKKAK HPFMNRESLI
310 320 330 340 350
VLADYVTLED GTGCVHTAPG HGAEDYLTGI KYNLPVLSPV NEEGVFTKDA
360 370 380 390 400
GKYAGLKIWD ANKVIIEDLE KLGVLIKSQK IEHSYPHCWR CKNPVIFRAT
410 420 430 440 450
PQWFISVDRN GLREKVLEEI KKVEWHPKWG ENRITAMVKE RPDWTISRQR
460 470 480 490 500
VWGTPIPAIK CKECGEVFLD EKVIDNFVNI VEEKGTDAWF ELSEDELIPE
510 520 530 540 550
GVKCPKCGKN SFEKTYDTLD VWIDSGCSFE AVIRSKGEKF PVDLYLEGDD
560 570 580 590 600
QHRGWFQSSI FMSVAHTNIA PYKSVVTHGF IKDEHGRKMS KSLGNVIDPK
610 620 630 640 650
EIVDKYGADI LRLWVSSIDF FDNIRVGKNI IQQQVETYKK LRNTLRYLLG
660 670 680 690 700
NLNDFEENMI VPFEKLLPLD KWALGRLQEV ISQVTEHFEK YEYSKVYSLL
710 720 730 740 750
NRYCTVELSA TYLDIIKDRI YVEAKGSIYR RSAQTVMYYI LQSLIKMLAP
760 770 780 790 800
ILVFTAEEAY QLSPFKKYET VHLEYWPEVK EEYIDNQIME DFKMILLIRD
810 820 830 840 850
DVLKALEEAR KQDIIGHSLD AKVTIEGLND NVQMIIEKHK DYLSEIFIVS
860 870 880 890 900
DVEIGNGQTK GEFVSVTVEK AKGEKCQRCW KYSEDVGKDD TYKDVCPRCA

AVLKGERK
Length:908
Mass (Da):105,172
Last modified:July 24, 2007 - v1
Checksum:iA10220901D34034F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA Translation: ABR30328.1
RefSeqiWP_012056689.1, NC_009616.1

Genome annotation databases

EnsemblBacteriaiABR30328; ABR30328; Tmel_0461
KEGGitme:Tmel_0461

Similar proteinsi

Entry informationi

Entry nameiSYI_THEM4
AccessioniPrimary (citable) accession number: A6LK77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 24, 2007
Last modified: March 28, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health