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A6LK77

- SYI_THEM4

UniProt

A6LK77 - SYI_THEM4

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermosipho melanesiensis (strain BI429 / DSM 12029)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (24 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei547 – 5471Aminoacyl-adenylateUniRule annotation
    Binding sitei591 – 5911ATPUniRule annotation
    Metal bindingi876 – 8761ZincUniRule annotation
    Metal bindingi879 – 8791ZincUniRule annotation
    Metal bindingi896 – 8961ZincUniRule annotation
    Metal bindingi899 – 8991ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTMEL391009:GHM1-485-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Tmel_0461
    OrganismiThermosipho melanesiensis (strain BI429 / DSM 12029)
    Taxonomic identifieri391009 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho
    ProteomesiUP000001110: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 908908Isoleucine--tRNA ligasePRO_1000022137Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi391009.Tmel_0461.

    Structurei

    3D structure databases

    ProteinModelPortaliA6LK77.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi588 – 5925"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6LK77-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKETLNLP STEFSMRANL VKKEPEMIKK WHEMDDYNLI LKSREGKPKF    50
    VLHDGPPYAN GNIHIGTATN KILKDIVIRY KTMRGYYAPY VPGWDTHGLP 100
    IEHRVSVEMG EKIKGMSPVE IRQKCKEFAL HFVDVQREQF KRLGVRGDWD 150
    NPYLTLDPKY EKHILNIFKT LVKNGNVYRG NKPVYWCPTC KTALAEAEVE 200
    YHDHSSPSIY VKFELEKNTY IVIWTTTPWT LPANVAIAVH PEYDYVKIKV 250
    GNEYWIVAEG LLNKFAAETE IEYSVVEKFK GKDLEHKKAK HPFMNRESLI 300
    VLADYVTLED GTGCVHTAPG HGAEDYLTGI KYNLPVLSPV NEEGVFTKDA 350
    GKYAGLKIWD ANKVIIEDLE KLGVLIKSQK IEHSYPHCWR CKNPVIFRAT 400
    PQWFISVDRN GLREKVLEEI KKVEWHPKWG ENRITAMVKE RPDWTISRQR 450
    VWGTPIPAIK CKECGEVFLD EKVIDNFVNI VEEKGTDAWF ELSEDELIPE 500
    GVKCPKCGKN SFEKTYDTLD VWIDSGCSFE AVIRSKGEKF PVDLYLEGDD 550
    QHRGWFQSSI FMSVAHTNIA PYKSVVTHGF IKDEHGRKMS KSLGNVIDPK 600
    EIVDKYGADI LRLWVSSIDF FDNIRVGKNI IQQQVETYKK LRNTLRYLLG 650
    NLNDFEENMI VPFEKLLPLD KWALGRLQEV ISQVTEHFEK YEYSKVYSLL 700
    NRYCTVELSA TYLDIIKDRI YVEAKGSIYR RSAQTVMYYI LQSLIKMLAP 750
    ILVFTAEEAY QLSPFKKYET VHLEYWPEVK EEYIDNQIME DFKMILLIRD 800
    DVLKALEEAR KQDIIGHSLD AKVTIEGLND NVQMIIEKHK DYLSEIFIVS 850
    DVEIGNGQTK GEFVSVTVEK AKGEKCQRCW KYSEDVGKDD TYKDVCPRCA 900
    AVLKGERK 908
    Length:908
    Mass (Da):105,172
    Last modified:July 24, 2007 - v1
    Checksum:iA10220901D34034F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000716 Genomic DNA. Translation: ABR30328.1.
    RefSeqiYP_001305713.1. NC_009616.1.

    Genome annotation databases

    EnsemblBacteriaiABR30328; ABR30328; Tmel_0461.
    GeneIDi5297673.
    KEGGitme:Tmel_0461.
    PATRICi23922081. VBITheMel19269_0486.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000716 Genomic DNA. Translation: ABR30328.1 .
    RefSeqi YP_001305713.1. NC_009616.1.

    3D structure databases

    ProteinModelPortali A6LK77.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391009.Tmel_0461.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR30328 ; ABR30328 ; Tmel_0461 .
    GeneIDi 5297673.
    KEGGi tme:Tmel_0461.
    PATRICi 23922081. VBITheMel19269_0486.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci TMEL391009:GHM1-485-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BI429 / DSM 12029.

    Entry informationi

    Entry nameiSYI_THEM4
    AccessioniPrimary (citable) accession number: A6LK77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3