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A6LK77

- SYI_THEM4

UniProt

A6LK77 - SYI_THEM4

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Tmel_0461
Organism
Thermosipho melanesiensis (strain BI429 / DSM 12029)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei547 – 5471Aminoacyl-adenylate By similarity
Binding sitei591 – 5911ATP By similarity
Metal bindingi876 – 8761Zinc By similarity
Metal bindingi879 – 8791Zinc By similarity
Metal bindingi896 – 8961Zinc By similarity
Metal bindingi899 – 8991Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTMEL391009:GHM1-485-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Tmel_0461
OrganismiThermosipho melanesiensis (strain BI429 / DSM 12029)
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho
ProteomesiUP000001110: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 908908Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022137Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi391009.Tmel_0461.

Structurei

3D structure databases

ProteinModelPortaliA6LK77.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi588 – 5925"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6LK77-1 [UniParc]FASTAAdd to Basket

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MDYKETLNLP STEFSMRANL VKKEPEMIKK WHEMDDYNLI LKSREGKPKF    50
VLHDGPPYAN GNIHIGTATN KILKDIVIRY KTMRGYYAPY VPGWDTHGLP 100
IEHRVSVEMG EKIKGMSPVE IRQKCKEFAL HFVDVQREQF KRLGVRGDWD 150
NPYLTLDPKY EKHILNIFKT LVKNGNVYRG NKPVYWCPTC KTALAEAEVE 200
YHDHSSPSIY VKFELEKNTY IVIWTTTPWT LPANVAIAVH PEYDYVKIKV 250
GNEYWIVAEG LLNKFAAETE IEYSVVEKFK GKDLEHKKAK HPFMNRESLI 300
VLADYVTLED GTGCVHTAPG HGAEDYLTGI KYNLPVLSPV NEEGVFTKDA 350
GKYAGLKIWD ANKVIIEDLE KLGVLIKSQK IEHSYPHCWR CKNPVIFRAT 400
PQWFISVDRN GLREKVLEEI KKVEWHPKWG ENRITAMVKE RPDWTISRQR 450
VWGTPIPAIK CKECGEVFLD EKVIDNFVNI VEEKGTDAWF ELSEDELIPE 500
GVKCPKCGKN SFEKTYDTLD VWIDSGCSFE AVIRSKGEKF PVDLYLEGDD 550
QHRGWFQSSI FMSVAHTNIA PYKSVVTHGF IKDEHGRKMS KSLGNVIDPK 600
EIVDKYGADI LRLWVSSIDF FDNIRVGKNI IQQQVETYKK LRNTLRYLLG 650
NLNDFEENMI VPFEKLLPLD KWALGRLQEV ISQVTEHFEK YEYSKVYSLL 700
NRYCTVELSA TYLDIIKDRI YVEAKGSIYR RSAQTVMYYI LQSLIKMLAP 750
ILVFTAEEAY QLSPFKKYET VHLEYWPEVK EEYIDNQIME DFKMILLIRD 800
DVLKALEEAR KQDIIGHSLD AKVTIEGLND NVQMIIEKHK DYLSEIFIVS 850
DVEIGNGQTK GEFVSVTVEK AKGEKCQRCW KYSEDVGKDD TYKDVCPRCA 900
AVLKGERK 908
Length:908
Mass (Da):105,172
Last modified:July 24, 2007 - v1
Checksum:iA10220901D34034F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000716 Genomic DNA. Translation: ABR30328.1.
RefSeqiYP_001305713.1. NC_009616.1.

Genome annotation databases

EnsemblBacteriaiABR30328; ABR30328; Tmel_0461.
GeneIDi5297673.
KEGGitme:Tmel_0461.
PATRICi23922081. VBITheMel19269_0486.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000716 Genomic DNA. Translation: ABR30328.1 .
RefSeqi YP_001305713.1. NC_009616.1.

3D structure databases

ProteinModelPortali A6LK77.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391009.Tmel_0461.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR30328 ; ABR30328 ; Tmel_0461 .
GeneIDi 5297673.
KEGGi tme:Tmel_0461.
PATRICi 23922081. VBITheMel19269_0486.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TMEL391009:GHM1-485-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BI429 / DSM 12029.

Entry informationi

Entry nameiSYI_THEM4
AccessioniPrimary (citable) accession number: A6LK77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 24, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi