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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermosipho melanesiensis (strain BI429 / DSM 12029)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei547 – 5471Aminoacyl-adenylateUniRule annotation
Binding sitei591 – 5911ATPUniRule annotation
Metal bindingi876 – 8761ZincUniRule annotation
Metal bindingi879 – 8791ZincUniRule annotation
Metal bindingi896 – 8961ZincUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTMEL391009:GHM1-485-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Tmel_0461
OrganismiThermosipho melanesiensis (strain BI429 / DSM 12029)
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho
ProteomesiUP000001110: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 908908Isoleucine--tRNA ligasePRO_1000022137Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391009.Tmel_0461.

Structurei

3D structure databases

ProteinModelPortaliA6LK77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi588 – 5925"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6LK77-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKETLNLP STEFSMRANL VKKEPEMIKK WHEMDDYNLI LKSREGKPKF
60 70 80 90 100
VLHDGPPYAN GNIHIGTATN KILKDIVIRY KTMRGYYAPY VPGWDTHGLP
110 120 130 140 150
IEHRVSVEMG EKIKGMSPVE IRQKCKEFAL HFVDVQREQF KRLGVRGDWD
160 170 180 190 200
NPYLTLDPKY EKHILNIFKT LVKNGNVYRG NKPVYWCPTC KTALAEAEVE
210 220 230 240 250
YHDHSSPSIY VKFELEKNTY IVIWTTTPWT LPANVAIAVH PEYDYVKIKV
260 270 280 290 300
GNEYWIVAEG LLNKFAAETE IEYSVVEKFK GKDLEHKKAK HPFMNRESLI
310 320 330 340 350
VLADYVTLED GTGCVHTAPG HGAEDYLTGI KYNLPVLSPV NEEGVFTKDA
360 370 380 390 400
GKYAGLKIWD ANKVIIEDLE KLGVLIKSQK IEHSYPHCWR CKNPVIFRAT
410 420 430 440 450
PQWFISVDRN GLREKVLEEI KKVEWHPKWG ENRITAMVKE RPDWTISRQR
460 470 480 490 500
VWGTPIPAIK CKECGEVFLD EKVIDNFVNI VEEKGTDAWF ELSEDELIPE
510 520 530 540 550
GVKCPKCGKN SFEKTYDTLD VWIDSGCSFE AVIRSKGEKF PVDLYLEGDD
560 570 580 590 600
QHRGWFQSSI FMSVAHTNIA PYKSVVTHGF IKDEHGRKMS KSLGNVIDPK
610 620 630 640 650
EIVDKYGADI LRLWVSSIDF FDNIRVGKNI IQQQVETYKK LRNTLRYLLG
660 670 680 690 700
NLNDFEENMI VPFEKLLPLD KWALGRLQEV ISQVTEHFEK YEYSKVYSLL
710 720 730 740 750
NRYCTVELSA TYLDIIKDRI YVEAKGSIYR RSAQTVMYYI LQSLIKMLAP
760 770 780 790 800
ILVFTAEEAY QLSPFKKYET VHLEYWPEVK EEYIDNQIME DFKMILLIRD
810 820 830 840 850
DVLKALEEAR KQDIIGHSLD AKVTIEGLND NVQMIIEKHK DYLSEIFIVS
860 870 880 890 900
DVEIGNGQTK GEFVSVTVEK AKGEKCQRCW KYSEDVGKDD TYKDVCPRCA

AVLKGERK
Length:908
Mass (Da):105,172
Last modified:July 24, 2007 - v1
Checksum:iA10220901D34034F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA. Translation: ABR30328.1.
RefSeqiWP_012056689.1. NC_009616.1.
YP_001305713.1. NC_009616.1.

Genome annotation databases

EnsemblBacteriaiABR30328; ABR30328; Tmel_0461.
GeneIDi5297673.
KEGGitme:Tmel_0461.
PATRICi23922081. VBITheMel19269_0486.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA. Translation: ABR30328.1.
RefSeqiWP_012056689.1. NC_009616.1.
YP_001305713.1. NC_009616.1.

3D structure databases

ProteinModelPortaliA6LK77.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391009.Tmel_0461.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR30328; ABR30328; Tmel_0461.
GeneIDi5297673.
KEGGitme:Tmel_0461.
PATRICi23922081. VBITheMel19269_0486.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciTMEL391009:GHM1-485-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BI429 / DSM 12029.

Entry informationi

Entry nameiSYI_THEM4
AccessioniPrimary (citable) accession number: A6LK77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 24, 2007
Last modified: January 7, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.