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A6LJM9 (SYL_THEM4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Tmel_0256
OrganismThermosipho melanesiensis (strain BI429 / DSM 12029) [Complete proteome] [HAMAP]
Taxonomic identifier391009 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho

Protein attributes

Sequence length823 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 823823Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000071115

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif580 – 5845"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5831ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6LJM9 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 3202B8451A516850

FASTA82395,319
        10         20         30         40         50         60 
MKEYIPGEIE PKWQKVWAES KVFETPQRSD KKKFYNLVMF PYPSGTLHVG HVKNYVIGDI 

        70         80         90        100        110        120 
VARYKRMKGY NVLHPFGYDA FGLPAENAAI KNKIHPEVWT FKNIDIIRNQ IKKIGISYDW 

       130        140        150        160        170        180 
SREVITCTEE YYKWTQWIFL KLYENGLAYK KKAAVNWCPS CQTVLANEQV VDGKCERCGT 

       190        200        210        220        230        240 
EVTMKHLEQW YFKITDYAEK LLNDIDKLRG WPENVKIMQK NWIGKSTGAE IDFPVDRLDM 

       250        260        270        280        290        300 
KIKVFTTRPD TIWGVTFMAI APESPLVEML VTDERKDELS QFLKKVSLED RFKRTSLEAE 

       310        320        330        340        350        360 
KEGFFLGRYA INPVTGEKIP IYVANYILYE YGTGAIMAVP AHDQRDFDFA KKYGISIRVV 

       370        380        390        400        410        420 
IDNPEESIDV EKMEKAYEEE GIMVNSGPFN GMRSTLALEK IIEYLEEKGI GKRSVQYKLR 

       430        440        450        460        470        480 
DWLISRQRYW GAPIPIVYCE KCGIVPVPEK DLPVKLPKDV EFLPTGQSPL SLDEQFLNTT 

       490        500        510        520        530        540 
CPKCGGPAKR EADTMDTFVD SSWYYLRYIN PKLEDKPFDT EDINYWMPVD QYIGGVEHAV 

       550        560        570        580        590        600 
LHLLYSRFIT KVLYDLGYLK FEEPFENLFT QGMIYKDGWK MSKSKGNVVS PDEMIEKYGA 

       610        620        630        640        650        660 
DTLRTYILFM APPEKDAEWS DAGIEGVNRF LKRLWNNIYS ILPRIKDVKV EKIELKNKQE 

       670        680        690        700        710        720 
KDLRRKLHQS IKKITEDIEG GFKFNTAIAG LMELNNNLSE YLNSAKDLNL PLLRELVEKL 

       730        740        750        760        770        780 
TLILSPFAPH MAEEIWHDLG NDTLVVNEEW PAYDENALKV DEVTVIIQIN GKVRGKIQTK 

       790        800        810        820 
VDVSEGEIKK LAFENAKIAS YVDGREIVKV IYVKNKLLNI VVK 

« Hide

References

[1]"Complete sequence of Thermosipho melanesiensis BI429."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BI429 / DSM 12029.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000716 Genomic DNA. Translation: ABR30130.1.
RefSeqYP_001305515.1. NC_009616.1.

3D structure databases

ProteinModelPortalA6LJM9.
SMRA6LJM9. Positions 1-759.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391009.Tmel_0256.

Proteomic databases

PRIDEA6LJM9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR30130; ABR30130; Tmel_0256.
GeneID5297116.
KEGGtme:Tmel_0256.
PATRIC23921623. VBITheMel19269_0265.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycTMEL391009:GHM1-269-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_THEM4
AccessionPrimary (citable) accession number: A6LJM9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 24, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries