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A6LJK6

- A6LJK6_THEM4

UniProt

A6LJK6 - A6LJK6_THEM4

Protein

Elongation factor 4

Gene

lepA

Organism
Thermosipho melanesiensis (strain BI429 / DSM 12029)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (24 Jul 2007)
      Previous versions | rss
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    Functioni

    Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

    Catalytic activityi

    GTP + H2O = GDP + phosphate.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 196GTPUniRule annotation
    Nucleotide bindingi132 – 1354GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. ribosome binding Source: UniProtKB-HAMAP
    4. translation elongation factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. positive regulation of translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    HydrolaseUniRule annotation

    Keywords - Biological processi

    Protein biosynthesisUniRule annotation

    Keywords - Ligandi

    GTP-bindingUniRule annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTMEL391009:GHM1-246-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
    Short name:
    EF-4UniRule annotation
    Alternative name(s):
    Ribosomal back-translocase LepAUniRule annotation
    Gene namesi
    Name:lepAUniRule annotation
    Ordered Locus Names:Tmel_0233Imported
    OrganismiThermosipho melanesiensis (strain BI429 / DSM 12029)Imported
    Taxonomic identifieri391009 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho
    ProteomesiUP000001110: Chromosome

    Subcellular locationi

    Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membraneUniRule annotation, Cell membrane, Membrane

    Interactioni

    Protein-protein interaction databases

    STRINGi391009.Tmel_0233.

    Structurei

    3D structure databases

    ProteinModelPortaliA6LJK6.
    SMRiA6LJK6. Positions 1-549.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GTP-binding elongation factor family. LepA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0481.
    HOGENOMiHOG000020624.
    KOiK03596.
    OMAiCYTALEM.
    OrthoDBiEOG6ZKXQ4.

    Family and domain databases

    Gene3Di3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00071. LepA.
    InterProiIPR006297. EF-4.
    IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR013842. LepA_GTP-bd_C.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00679. EFG_C. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF06421. LepA_C. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SMARTiSM00838. EFG_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsiTIGR01393. lepA. 1 hit.
    TIGR00231. small_GTP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A6LJK6-1 [UniParc]FASTAAdd to Basket

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    MKNVRNISII AHIDHGKTTL SDRILEITGA VEKRKMREQF LDSMDIERER    50
    GITIKSHPLR VFYKSKKNGK VYEINIVDTP GHVDFTYEVD RSLAAVEGVI 100
    LLVDASQGVQ AQTVANAYKA IEHNLEIIPV INKIDLPNAN IPETELEIED 150
    LIGISSEEIL KVSAKEGIGV EDVLEAIIGR VPSPKGTENE KLSALIFDAK 200
    YDKYRGVITY VRVFNGEIKP GDKIMTYSNK QVYEVVETGV FTPDMTPIEK 250
    LKAGDIGYII AGIKEVSLAK IGDTITNATD PVEEPLPGYK EIKPMVFAGM 300
    YPGVPEYYEE LRKALEKLKL NDSSLTFYPD HSPALGFGFR CGFLGLLHMD 350
    VVRERLEREF EMTVILTAPN VEYKVILKNG EEVIVNDPAK FPDESMIQEV 400
    YEPYVKLSII TPPEYLGKLM NLVQNEKRGI MTSTENAGFE RVVLNFEVPL 450
    AEIIFDFFDK MKASSRGYAS MDYEMIGYRK SELVKITILV NKEPVDALSI 500
    IAHKNKAYAM ARKLVDKLAE LIPQHQFEIP VQARAGGRII ARSTIKALRK 550
    DVLAKCYGGD VTRKMKLLQK QKEGKKRLRE IGSVSIPQEA FLALLKVGED 600
    ENK 603
    Length:603
    Mass (Da):67,748
    Last modified:July 24, 2007 - v1
    Checksum:iF9C84D751045143E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000716 Genomic DNA. Translation: ABR30107.1.
    RefSeqiYP_001305492.1. NC_009616.1.

    Genome annotation databases

    EnsemblBacteriaiABR30107; ABR30107; Tmel_0233.
    GeneIDi5298035.
    KEGGitme:Tmel_0233.
    PATRICi23921577. VBITheMel19269_0242.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000716 Genomic DNA. Translation: ABR30107.1 .
    RefSeqi YP_001305492.1. NC_009616.1.

    3D structure databases

    ProteinModelPortali A6LJK6.
    SMRi A6LJK6. Positions 1-549.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391009.Tmel_0233.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR30107 ; ABR30107 ; Tmel_0233 .
    GeneIDi 5298035.
    KEGGi tme:Tmel_0233.
    PATRICi 23921577. VBITheMel19269_0242.

    Phylogenomic databases

    eggNOGi COG0481.
    HOGENOMi HOG000020624.
    KOi K03596.
    OMAi CYTALEM.
    OrthoDBi EOG6ZKXQ4.

    Enzyme and pathway databases

    BioCyci TMEL391009:GHM1-246-MONOMER.

    Family and domain databases

    Gene3Di 3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00071. LepA.
    InterProi IPR006297. EF-4.
    IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR013842. LepA_GTP-bd_C.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00679. EFG_C. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF06421. LepA_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SMARTi SM00838. EFG_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsi TIGR01393. lepA. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BI429 / DSM 12029Imported.

    Entry informationi

    Entry nameiA6LJK6_THEM4
    AccessioniPrimary (citable) accession number: A6LJK6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 24, 2007
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3