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A6LJK6

- A6LJK6_THEM4

UniProt

A6LJK6 - A6LJK6_THEM4

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Protein
Elongation factor 4
Gene
lepA, Tmel_0233
Organism
Thermosipho melanesiensis (strain BI429 / DSM 12029)
Status
Unreviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 196GTP By similarityUniRule annotation
Nucleotide bindingi132 – 1354GTP By similarityUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. GTPase activity Source: UniProtKB-HAMAP
  3. ribosome binding Source: UniProtKB-HAMAP
  4. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. positive regulation of translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation

Keywords - Biological processi

Protein biosynthesisUniRule annotation

Keywords - Ligandi

GTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciTMEL391009:GHM1-246-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepA
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:Tmel_0233Imported
OrganismiThermosipho melanesiensis (strain BI429 / DSM 12029)Imported
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermosipho
ProteomesiUP000001110: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membraneUniRule annotation, Cell membrane, Membrane

Interactioni

Protein-protein interaction databases

STRINGi391009.Tmel_0233.

Structurei

3D structure databases

ProteinModelPortaliA6LJK6.
SMRiA6LJK6. Positions 1-549.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0481.
HOGENOMiHOG000020624.
KOiK03596.
OMAiCYTALEM.
OrthoDBiEOG6ZKXQ4.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6LJK6-1 [UniParc]FASTAAdd to Basket

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MKNVRNISII AHIDHGKTTL SDRILEITGA VEKRKMREQF LDSMDIERER    50
GITIKSHPLR VFYKSKKNGK VYEINIVDTP GHVDFTYEVD RSLAAVEGVI 100
LLVDASQGVQ AQTVANAYKA IEHNLEIIPV INKIDLPNAN IPETELEIED 150
LIGISSEEIL KVSAKEGIGV EDVLEAIIGR VPSPKGTENE KLSALIFDAK 200
YDKYRGVITY VRVFNGEIKP GDKIMTYSNK QVYEVVETGV FTPDMTPIEK 250
LKAGDIGYII AGIKEVSLAK IGDTITNATD PVEEPLPGYK EIKPMVFAGM 300
YPGVPEYYEE LRKALEKLKL NDSSLTFYPD HSPALGFGFR CGFLGLLHMD 350
VVRERLEREF EMTVILTAPN VEYKVILKNG EEVIVNDPAK FPDESMIQEV 400
YEPYVKLSII TPPEYLGKLM NLVQNEKRGI MTSTENAGFE RVVLNFEVPL 450
AEIIFDFFDK MKASSRGYAS MDYEMIGYRK SELVKITILV NKEPVDALSI 500
IAHKNKAYAM ARKLVDKLAE LIPQHQFEIP VQARAGGRII ARSTIKALRK 550
DVLAKCYGGD VTRKMKLLQK QKEGKKRLRE IGSVSIPQEA FLALLKVGED 600
ENK 603
Length:603
Mass (Da):67,748
Last modified:July 24, 2007 - v1
Checksum:iF9C84D751045143E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000716 Genomic DNA. Translation: ABR30107.1.
RefSeqiYP_001305492.1. NC_009616.1.

Genome annotation databases

EnsemblBacteriaiABR30107; ABR30107; Tmel_0233.
GeneIDi5298035.
KEGGitme:Tmel_0233.
PATRICi23921577. VBITheMel19269_0242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000716 Genomic DNA. Translation: ABR30107.1 .
RefSeqi YP_001305492.1. NC_009616.1.

3D structure databases

ProteinModelPortali A6LJK6.
SMRi A6LJK6. Positions 1-549.
ModBasei Search...

Protein-protein interaction databases

STRINGi 391009.Tmel_0233.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR30107 ; ABR30107 ; Tmel_0233 .
GeneIDi 5298035.
KEGGi tme:Tmel_0233.
PATRICi 23921577. VBITheMel19269_0242.

Phylogenomic databases

eggNOGi COG0481.
HOGENOMi HOG000020624.
KOi K03596.
OMAi CYTALEM.
OrthoDBi EOG6ZKXQ4.

Enzyme and pathway databases

BioCyci TMEL391009:GHM1-246-MONOMER.

Family and domain databases

Gene3Di 3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00071. LepA.
InterProi IPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SMARTi SM00838. EFG_C. 1 hit.
[Graphical view ]
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsi TIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BI429 / DSM 12029.

Entry informationi

Entry nameiA6LJK6_THEM4
AccessioniPrimary (citable) accession number: A6LJK6
Entry historyi
Integrated into UniProtKB/TrEMBL: July 24, 2007
Last sequence update: July 24, 2007
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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