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Protein

Elongation factor 4

Gene

lepA

Organism
Thermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 19GTPUniRule annotation6
Nucleotide bindingi132 – 135GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation
Biological processProtein biosynthesisUniRule annotation
LigandGTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciTMEL391009:G1G91-251-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:Tmel_0233Imported
OrganismiThermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)Imported
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeThermosipho
Proteomesi
  • UP000001110 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membraneUniRule annotation, Cell membrane, Membrane

Interactioni

Protein-protein interaction databases

STRINGi391009.Tmel_0233

Structurei

3D structure databases

ProteinModelPortaliA6LJK6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 185Tr-type GInterPro annotationAdd BLAST184

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S Bacteria
COG0481 LUCA
HOGENOMiHOG000020624
KOiK03596
OMAiALIFDSW
OrthoDBiPOG091H02HW

Family and domain databases

CDDicd03709 lepA_C, 1 hit
Gene3Di3.30.70.2570, 1 hit
HAMAPiMF_00071 LepA, 1 hit
InterProiView protein in InterPro
IPR006297 EF-4
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR038363 LepA_C_sf
IPR013842 LepA_CTD
IPR035654 LepA_IV
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF06421 LepA_C, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR01393 lepA, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

A6LJK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNVRNISII AHIDHGKTTL SDRILEITGA VEKRKMREQF LDSMDIERER
60 70 80 90 100
GITIKSHPLR VFYKSKKNGK VYEINIVDTP GHVDFTYEVD RSLAAVEGVI
110 120 130 140 150
LLVDASQGVQ AQTVANAYKA IEHNLEIIPV INKIDLPNAN IPETELEIED
160 170 180 190 200
LIGISSEEIL KVSAKEGIGV EDVLEAIIGR VPSPKGTENE KLSALIFDAK
210 220 230 240 250
YDKYRGVITY VRVFNGEIKP GDKIMTYSNK QVYEVVETGV FTPDMTPIEK
260 270 280 290 300
LKAGDIGYII AGIKEVSLAK IGDTITNATD PVEEPLPGYK EIKPMVFAGM
310 320 330 340 350
YPGVPEYYEE LRKALEKLKL NDSSLTFYPD HSPALGFGFR CGFLGLLHMD
360 370 380 390 400
VVRERLEREF EMTVILTAPN VEYKVILKNG EEVIVNDPAK FPDESMIQEV
410 420 430 440 450
YEPYVKLSII TPPEYLGKLM NLVQNEKRGI MTSTENAGFE RVVLNFEVPL
460 470 480 490 500
AEIIFDFFDK MKASSRGYAS MDYEMIGYRK SELVKITILV NKEPVDALSI
510 520 530 540 550
IAHKNKAYAM ARKLVDKLAE LIPQHQFEIP VQARAGGRII ARSTIKALRK
560 570 580 590 600
DVLAKCYGGD VTRKMKLLQK QKEGKKRLRE IGSVSIPQEA FLALLKVGED

ENK
Length:603
Mass (Da):67,748
Last modified:July 24, 2007 - v1
Checksum:iF9C84D751045143E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA Translation: ABR30107.1
RefSeqiWP_012056468.1, NC_009616.1

Genome annotation databases

EnsemblBacteriaiABR30107; ABR30107; Tmel_0233
KEGGitme:Tmel_0233

Similar proteinsi

Entry informationi

Entry nameiA6LJK6_THEM4
AccessioniPrimary (citable) accession number: A6LJK6
Entry historyiIntegrated into UniProtKB/TrEMBL: July 24, 2007
Last sequence update: July 24, 2007
Last modified: March 28, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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