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Protein

Elongation factor 4

Gene

lepA

Organism
Thermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 19GTPUniRule annotation6
Nucleotide bindingi132 – 135GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation
Biological processProtein biosynthesisUniRule annotation
LigandGTP-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:Tmel_0233Imported
OrganismiThermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)Imported
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeThermosipho
Proteomesi
  • UP000001110 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membraneUniRule annotation, Cell membrane, Membrane

Interactioni

Protein-protein interaction databases

STRINGi391009.Tmel_0233.

Structurei

3D structure databases

ProteinModelPortaliA6LJK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 185Tr-type GInterPro annotationAdd BLAST184

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
HOGENOMiHOG000020624.
KOiK03596.
OMAiALIFDSW.
OrthoDBiPOG091H02HW.

Family and domain databases

CDDicd03709. lepA_C. 1 hit.
HAMAPiMF_00071. LepA. 1 hit.
InterProiView protein in InterPro
IPR006297. EF-4.
IPR035647. EFG_III/V.
IPR000640. EFG_V-like.
IPR004161. EFTu-like_2.
IPR013842. LepA_CTD.
IPR035654. LepA_IV.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
PfamiView protein in Pfam
PF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
PRINTSiPR00315. ELONGATNFCT.
SMARTiView protein in SMART
SM00838. EFG_C. 1 hit.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51722. G_TR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A6LJK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNVRNISII AHIDHGKTTL SDRILEITGA VEKRKMREQF LDSMDIERER
60 70 80 90 100
GITIKSHPLR VFYKSKKNGK VYEINIVDTP GHVDFTYEVD RSLAAVEGVI
110 120 130 140 150
LLVDASQGVQ AQTVANAYKA IEHNLEIIPV INKIDLPNAN IPETELEIED
160 170 180 190 200
LIGISSEEIL KVSAKEGIGV EDVLEAIIGR VPSPKGTENE KLSALIFDAK
210 220 230 240 250
YDKYRGVITY VRVFNGEIKP GDKIMTYSNK QVYEVVETGV FTPDMTPIEK
260 270 280 290 300
LKAGDIGYII AGIKEVSLAK IGDTITNATD PVEEPLPGYK EIKPMVFAGM
310 320 330 340 350
YPGVPEYYEE LRKALEKLKL NDSSLTFYPD HSPALGFGFR CGFLGLLHMD
360 370 380 390 400
VVRERLEREF EMTVILTAPN VEYKVILKNG EEVIVNDPAK FPDESMIQEV
410 420 430 440 450
YEPYVKLSII TPPEYLGKLM NLVQNEKRGI MTSTENAGFE RVVLNFEVPL
460 470 480 490 500
AEIIFDFFDK MKASSRGYAS MDYEMIGYRK SELVKITILV NKEPVDALSI
510 520 530 540 550
IAHKNKAYAM ARKLVDKLAE LIPQHQFEIP VQARAGGRII ARSTIKALRK
560 570 580 590 600
DVLAKCYGGD VTRKMKLLQK QKEGKKRLRE IGSVSIPQEA FLALLKVGED

ENK
Length:603
Mass (Da):67,748
Last modified:July 24, 2007 - v1
Checksum:iF9C84D751045143E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA. Translation: ABR30107.1.
RefSeqiWP_012056468.1. NC_009616.1.

Genome annotation databases

EnsemblBacteriaiABR30107; ABR30107; Tmel_0233.
KEGGitme:Tmel_0233.

Similar proteinsi

Entry informationi

Entry nameiA6LJK6_THEM4
AccessioniPrimary (citable) accession number: A6LJK6
Entry historyiIntegrated into UniProtKB/TrEMBL: July 24, 2007
Last sequence update: July 24, 2007
Last modified: September 27, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported