A6LJ42 (SYE1_THEM4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Tmel_0065

Organism

Thermosipho melanesiensis (strain BI429 / DSM 12029) [Complete proteome] [HAMAP]

Taxonomic identifier

391009 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermosipho

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367786
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	250 – 254	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	253	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6LJ42 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 0D7982A16D2125D1	FASTA	473	55,265
	10 20 30 40 50 60 MVRLRFAPSP TGYLHVGGAR TALFNFLYAR KMKGSFILRI EDTDLERSQK EYEEGLINAL 70 80 90 100 110 120 KWLGLEWDEG PDVGGDFGPY RQSERLEIYW KYARKLIEEG KAYEVYAYPE EIEKLREKLL 130 140 150 160 170 180 AQGKAPHYTR EMLQQFTTPE RIKEYEKKGL KPAIYFEMPR KEYVLNDIVK GRVIFKKGTV 190 200 210 220 230 240 GDFAIIRSNG VPIYNFACVI DDHLMNITHV LRGDDHLSNT VKQIALYEAF NWETPEFGHV 250 260 270 280 290 300 SMILGPDAKK LSKRHGATSV EEFRDRGYLP QAVVNFLALL GWSHPEGKEI MNLNELIDAF 310 320 330 340 350 360 SLDRLGKNPA IFDPKKLRWM NAEHFRNLSE DKMLDVSRKY LLKFVTEDEI DSRKDWFIRL 370 380 390 400 410 420 LKSIKDRIEE LSEIPNLVEF FFLEPDVTVD LSDEVKEVYL RLIEEFEEVE EWNEKNILNS 430 440 450 460 470 FKAAMKGSKV KGKEFYMKLR IVLTGREEGP ELIDVVYLLG KENIIKRLKK HLG

References

[1]

"Complete sequence of Thermosipho melanesiensis BI429." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BI429 / DSM 12029.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000716 Genomic DNA. Translation: ABR29943.1.
RefSeq	YP_001305328.1. NC_009616.1.
3D structure databases
ProteinModelPortal	A6LJ42.
SMR	A6LJ42. Positions 1-473.
ModBase	Search...
Protein-protein interaction databases
STRING	A6LJ42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5296716.
GenomeReviews	Gene locus Tmel_0065 in contig CP000716_GR.
KEGG	tme:Tmel_0065.
NMPDR	fig|391009.4.peg.70.
PATRIC	23921215. VBITheMel19269_0069.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	AYEVYAY.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K09698.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_THEM4

Accession

Primary (citable) accession number: A6LJ42

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 24, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program