A6LIT0 (QUEF_PARD8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	BDI_3913

Organism

Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152) [Complete proteome] [HAMAP]

Taxonomic identifier

435591 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Parabacteroides

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm By similarity HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_1000213096

Sequences

	Sequence		Length	Mass (Da)
	A6LIT0 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 7F2682E23F09F289	FASTA	157	18,335
	10 20 30 40 50 60 MKEMDERKQE GELHLLGGST VYKQDYAPEV LEAFTNKHQG NDYWVRFNCP EFTSLCPITG 70 80 90 100 110 120 QPDFATIHID YIPDVKMVES KSLKLYLFSF RNHGAFHEDC VNIIMKDLIK LMDPKYIEIT 130 140 150 GIFTPRGGIS IYPYCNYGRP NTKFEQLAEK RLFDHNQ

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8503 / DSM 20701 / NCTC 11152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000140 Genomic DNA. Translation: ABR45594.1.
RefSeq	YP_001305216.1. NC_009615.1.
3D structure databases
ProteinModelPortal	A6LIT0.
ModBase	Search...
Protein-protein interaction databases
STRING	A6LIT0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5309062.
GenomeReviews	Gene locus BDI_3913 in contig CP000140_GR.
KEGG	pdi:BDI_3913.
PATRIC	22851068. VBIParDis29947_3867.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG294103.
OMA	CINTILL.
ProtClustDB	PRK13258.
Enzyme and pathway databases
BioCyc	PDIS435591:BDI_3913-MONOMER.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K09457.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_PARD8

Accession

Primary (citable) accession number: A6LIT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program