A6LFT2 (GLAB_PARD8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase B
EC=3.2.1.n1
EC=3.2.1.n2

Alternative name(s):
Exo-alpha-galactosidase B
EC=3.2.1.22

Gene names

Name:	glaB
Ordered Locus Names:	BDI_2833

Organism

Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152) [Complete proteome] [HAMAP]

Taxonomic identifier

435591 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Parabacteroides

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Hydrolysis of terminal, non-reducing linear (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Subcellular location	Cell membrane; Lipid-anchor Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. B subfamily. Contains 3 PbH1 repeats.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 612	593	Alpha-1,3-galactosidase B		PRO_0000348481
Regions
	Repeat	431 – 453	23	PbH1 1
	Repeat	454 – 476	23	PbH1 2
	Repeat	487 – 536	50	PbH1 3
Amino acid modifications
	Lipidation	20	1	N-palmitoyl cysteine Potential
	Lipidation	20	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	A6LFT2 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: D9F7986EB522590C	FASTA	612	69,565
	10 20 30 40 50 60 MKWYLWGAVV LLYSLFGSAC STEWRYDLSA YGLSPVENVD NAPAMARALE QIREKCEENQ 70 80 90 100 110 120 TIVVTLPKGR YEFYPDSAAE RVYFISNHDQ MNPKKVGLPF EGMKNMVFDG QGSELIFHGR 130 140 150 160 170 180 MLPVSLLDSR NCVLKNFSID FKHPQISQVK VVENDTVNGG ITFEVAPWVH YEIRDSVFVA 190 200 210 220 230 240 KGEGWELTPG SGIAFEGDTR HLVYNTSDIP VGVRGLIEVS PRLIKSPRWK DNRLVPGTVI 250 260 270 280 290 300 AMRSWERPAP GVFLYHDVNT TLENIKVHYA EGMGLLAQMS ENITLDGFSV CLKGADDPRY 310 320 330 340 350 360 FTTQADATHF SACKGAIISK NGLYEGMMDD AINVHGTYLK VVRRVNDSTL VGRYMHPQSY 370 380 390 400 410 420 GFEWGRVGDS VQFIHSSTME LIGARNRITA IKAVDQPDYR GAKEFEIRFE NTVNPSIHEG 430 440 450 460 470 480 SGFGIENLEW TPTVLFSDNL IRNNRARGSL FSTPRQTVVE NNVFDHTSGT AILLCGDCNG 490 500 510 520 530 540 WFETGACRNV LIRKNKFINS LTNMFQFTNA IISIYPEIPD LASQRKYFHS DIVIDANEFI 550 560 570 580 590 600 TFDRPLVYAK SVDGLVFTNN IVKQNKEYPA FHWNNYRFYF QRVIHSKIEN NYFDEGFIRE 610 RDVLEENNGY DI

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8503 / DSM 20701 / NCTC 11152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000140 Genomic DNA. Translation: ABR44546.1.
RefSeq	YP_001304168.1. NC_009615.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6LFT2.
Protein family/group databases
CAZy	GH110. Glycoside Hydrolase Family 110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5307982.
GenomeReviews	Gene locus BDI_2833 in contig CP000140_GR.
KEGG	pdi:BDI_2833.
PATRIC	22848878. VBIParDis29947_2802.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG77539.
HOGENOM	HBG345842.
OMA	LTWTPEV.
ProtClustDB	CLSK823424.
Enzyme and pathway databases
BioCyc	PDIS435591:BDI_2833-MONOMER.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 3 hits.
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLAB_PARD8

Accession

Primary (citable) accession number: A6LFT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program